Interaction of micron and nano-sized particles with cells of the dura mater.

Papageorgiou, I; Marsh, R; Tipper, JL; Hall, RM; Fisher, J; Ingham, E

Interaction of micron and nano-sized particles with cells of the dura mater.

Papageorgiou, I Marsh, R Tipper, JL Hall, RM Fisher, J Ingham, E

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Publisher:: WILEY
Publication Type:: Journal Article
Citation:: Journal of biomedical materials research. Part B, Applied biomaterials, 2014, 102, (7), pp. 1496-1505
Issue Date:: 2014-10

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Field	Value	Language
dc.contributor.author	Papageorgiou, I
dc.contributor.author	Marsh, R
dc.contributor.author	Tipper, JL
dc.contributor.author	Hall, RM
dc.contributor.author	Fisher, J
dc.contributor.author	Ingham, E
dc.date.accessioned	2021-07-21T07:26:42Z
dc.date.available	2014-02-18
dc.date.available	2021-07-21T07:26:42Z
dc.date.issued	2014-10
dc.identifier.citation	Journal of biomedical materials research. Part B, Applied biomaterials, 2014, 102, (7), pp. 1496-1505
dc.identifier.issn	1552-4973
dc.identifier.issn	1552-4981
dc.identifier.uri	http://hdl.handle.net/10453/149907
dc.description.abstract	Intervertebral total disc replacements (TDR) are used in the treatment of degenerative spinal disc disease. There are, however, concerns that they may be subject to long-term failure due to wear. The adverse effects of TDR wear have the potential to manifest in the dura mater and surrounding tissues. The aim of this study was to investigate the physiological structure of the dura mater, isolate the resident dural epithelial and stromal cells and analyse the capacity of these cells to internalise model polymer particles. The porcine dura mater was a collagen-rich structure encompassing regularly arranged fibroblastic cells within an outermost epithelial cell layer. The isolated dural epithelial cells had endothelial cell characteristics (positive for von Willebrand factor, CD31, E-cadherin and desmoplakin) and barrier functionality whereas the fibroblastic cells were positive for collagen I and III, tenascin and actin. The capacity of the dural cells to take up model particles was dependent on particle size. Nanometer sized particles readily penetrated both types of cells. However, dural fibroblasts engulfed micron-sized particles at a much higher rate than dural epithelial cells. The study suggested that dural epithelial cells may offer some barrier to the penetration of micron-sized particles but not nanometer sized particles.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	WILEY
dc.relation.ispartof	Journal of biomedical materials research. Part B, Applied biomaterials
dc.relation.isbasedon	10.1002/jbm.b.33129
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0903 Biomedical Engineering, 0912 Materials Engineering
dc.subject.classification	Biomedical Engineering
dc.subject.mesh	Dura Mater
dc.subject.mesh	Fibroblasts
dc.subject.mesh	Epithelial Cells
dc.subject.mesh	Animals
dc.subject.mesh	Swine
dc.subject.mesh	Particle Size
dc.subject.mesh	Nanoparticles
dc.subject.mesh	Total Disc Replacement
dc.subject.mesh	Animals
dc.subject.mesh	Dura Mater
dc.subject.mesh	Epithelial Cells
dc.subject.mesh	Fibroblasts
dc.subject.mesh	Nanoparticles
dc.subject.mesh	Particle Size
dc.subject.mesh	Swine
dc.subject.mesh	Total Disc Replacement
dc.title	Interaction of micron and nano-sized particles with cells of the dura mater.
dc.type	Journal Article
utslib.citation.volume	102
utslib.location.activity	United States
utslib.for	0903 Biomedical Engineering
utslib.for	0912 Materials Engineering
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	open_access	*
dc.date.updated	2021-07-21T07:26:39Z
pubs.issue	7
pubs.publication-status	Published
pubs.volume	102
utslib.citation.issue	7

Abstract:

Intervertebral total disc replacements (TDR) are used in the treatment of degenerative spinal disc disease. There are, however, concerns that they may be subject to long-term failure due to wear. The adverse effects of TDR wear have the potential to manifest in the dura mater and surrounding tissues. The aim of this study was to investigate the physiological structure of the dura mater, isolate the resident dural epithelial and stromal cells and analyse the capacity of these cells to internalise model polymer particles. The porcine dura mater was a collagen-rich structure encompassing regularly arranged fibroblastic cells within an outermost epithelial cell layer. The isolated dural epithelial cells had endothelial cell characteristics (positive for von Willebrand factor, CD31, E-cadherin and desmoplakin) and barrier functionality whereas the fibroblastic cells were positive for collagen I and III, tenascin and actin. The capacity of the dural cells to take up model particles was dependent on particle size. Nanometer sized particles readily penetrated both types of cells. However, dural fibroblasts engulfed micron-sized particles at a much higher rate than dural epithelial cells. The study suggested that dural epithelial cells may offer some barrier to the penetration of micron-sized particles but not nanometer sized particles.

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http://hdl.handle.net/10453/149907