Studying Mitochondrial Network Formation by In Vivo and In Vitro Reconstitution Assay.

Du, W; Di, X; Su, QP

Studying Mitochondrial Network Formation by In Vivo and In Vitro Reconstitution Assay.

Du, W Di, X Su, QP

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Publisher:: Springer US
Publication Type:: Chapter
Citation:: Methods in Molecular Biology, 2021, 2276, pp. 333-341
Issue Date:: 2021-01

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Field	Value	Language
dc.contributor.author	Du, W
dc.contributor.author	Di, X
dc.contributor.author	Su, QP
dc.date.accessioned	2021-08-24T05:17:05Z
dc.date.available	2021-08-24T05:17:05Z
dc.date.issued	2021-01
dc.identifier.citation	Methods in Molecular Biology, 2021, 2276, pp. 333-341
dc.identifier.uri	http://hdl.handle.net/10453/150220
dc.description.abstract	Mitochondria change their morphologies from small isolated vesicles to large continuous networks across the cell cycles. The mitochondrial network formation (MNF) plays an important role in maintaining mitochondrial DNA integrity and interchanging mitochondrial materials. The disruption of the mitochondrial network affects mitochondrial functions, such as ATP production, integration of metabolism, calcium homeostasis, and regulation of apoptosis, leading to the abnormal development and several human diseases including neurodegenerative disease. In this unit, we describe the method of studying MNF, which is driven by microtubule-dependent motor protein, by in vivo imaging and single-molecule in vitro reconstitution assays.
dc.format	Print
dc.language	en
dc.publisher	Springer US
dc.relation.ispartof	Methods in Molecular Biology
dc.relation.isbasedon	10.1007/978-1-0716-1266-8_25
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0399 Other Chemical Sciences, 0601 Biochemistry and Cell Biology
dc.subject.classification	Developmental Biology
dc.subject.mesh	Cells, Cultured
dc.subject.mesh	Microtubules
dc.subject.mesh	Mitochondria
dc.subject.mesh	Animals
dc.subject.mesh	Mice
dc.subject.mesh	Rats
dc.subject.mesh	Kinesin
dc.subject.mesh	DNA, Mitochondrial
dc.subject.mesh	Microscopy, Fluorescence
dc.subject.mesh	Membrane Potential, Mitochondrial
dc.subject.mesh	Mitochondrial Dynamics
dc.subject.mesh	In Vitro Techniques
dc.subject.mesh	Animals
dc.subject.mesh	Cells, Cultured
dc.subject.mesh	DNA, Mitochondrial
dc.subject.mesh	In Vitro Techniques
dc.subject.mesh	Kinesin
dc.subject.mesh	Membrane Potential, Mitochondrial
dc.subject.mesh	Mice
dc.subject.mesh	Microscopy, Fluorescence
dc.subject.mesh	Microtubules
dc.subject.mesh	Mitochondria
dc.subject.mesh	Mitochondrial Dynamics
dc.subject.mesh	Rats
dc.title	Studying Mitochondrial Network Formation by In Vivo and In Vitro Reconstitution Assay.
dc.type	Chapter
utslib.citation.volume	2276
utslib.for	0399 Other Chemical Sciences
utslib.for	0601 Biochemistry and Cell Biology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	closed_access	*
dc.date.updated	2021-08-24T05:17:04Z
pubs.publication-status	Published
pubs.volume	2276

Abstract:

Mitochondria change their morphologies from small isolated vesicles to large continuous networks across the cell cycles. The mitochondrial network formation (MNF) plays an important role in maintaining mitochondrial DNA integrity and interchanging mitochondrial materials. The disruption of the mitochondrial network affects mitochondrial functions, such as ATP production, integration of metabolism, calcium homeostasis, and regulation of apoptosis, leading to the abnormal development and several human diseases including neurodegenerative disease. In this unit, we describe the method of studying MNF, which is driven by microtubule-dependent motor protein, by in vivo imaging and single-molecule in vitro reconstitution assays.

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http://hdl.handle.net/10453/150220