Towards engineering heart tissues from bioprinted cardiac spheroids.

Polonchuk, L; Surija, L; Lee, MH; Sharma, P; Liu Chung Ming, C; Richter, F; Ben-Sefer, E; Alsadat Rad, M; Mahmodi Sheikh Sarmast, H; Al Shamery, W; Tran, HA; Vettori, L; Haeusermann, F; Filipe, EC; Rnjak-Kovacina, J; Cox, T; Tipper, J; Kabakova, I; Gentile, C

Towards engineering heart tissues from bioprinted cardiac spheroids.

Polonchuk, L Surija, L Lee, MH Sharma, P Liu Chung Ming, C Richter, F Ben-Sefer, E Alsadat Rad, M Mahmodi Sheikh Sarmast, H

Al Shamery, W Tran, HA Vettori, L Haeusermann, F Filipe, EC Rnjak-Kovacina, J Cox, T Tipper, J Kabakova, I

Gentile, C

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Publisher:: IOP PUBLISHING LTD
Publication Type:: Journal Article
Citation:: Biofabrication, 2021, 13, (4)
Issue Date:: 2021-07-15

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Field	Value	Language
dc.contributor.author	Polonchuk, L
dc.contributor.author	Surija, L
dc.contributor.author	Lee, MH
dc.contributor.author	Sharma, P
dc.contributor.author	Liu Chung Ming, C
dc.contributor.author	Richter, F
dc.contributor.author	Ben-Sefer, E
dc.contributor.author	Alsadat Rad, M
dc.contributor.author	Mahmodi Sheikh Sarmast, H https://orcid.org/0000-0003-4820-5614
dc.contributor.author	Al Shamery, W
dc.contributor.author	Tran, HA
dc.contributor.author	Vettori, L
dc.contributor.author	Haeusermann, F
dc.contributor.author	Filipe, EC
dc.contributor.author	Rnjak-Kovacina, J
dc.contributor.author	Cox, T
dc.contributor.author	Tipper, J
dc.contributor.author	Kabakova, I https://orcid.org/0000-0002-6831-9478
dc.contributor.author	Gentile, C https://orcid.org/0000-0002-3689-4275
dc.date.accessioned	2021-11-29T11:02:47Z
dc.date.available	2021-07-15
dc.date.available	2021-11-29T11:02:47Z
dc.date.issued	2021-07-15
dc.identifier.citation	Biofabrication, 2021, 13, (4)
dc.identifier.issn	1758-5082
dc.identifier.issn	1758-5090
dc.identifier.uri	http://hdl.handle.net/10453/151913
dc.description.abstract	Current<i>in vivo</i>and<i>in vitro</i>models fail to accurately recapitulate the human heart microenvironment for biomedical applications. This study explores the use of cardiac spheroids (CSs) to biofabricate advanced<i>in vitro</i>models of the human heart. CSs were created from human cardiac myocytes, fibroblasts and endothelial cells (ECs), mixed within optimal alginate/gelatin hydrogels and then bioprinted on a microelectrode plate for drug testing. Bioprinted CSs maintained their structure and viability for at least 30 d after printing. Vascular endothelial growth factor (VEGF) promoted EC branching from CSs within hydrogels. Alginate/gelatin-based hydrogels enabled spheroids fusion, which was further facilitated by addition of VEGF. Bioprinted CSs contracted spontaneously and under stimulation, allowing to record contractile and electrical signals on the microelectrode plates for industrial applications. Taken together, our findings indicate that bioprinted CSs can be used to biofabricate human heart tissues for long term<i>in vitro</i>testing. This has the potential to be used to study biochemical, physiological and pharmacological features of human heart tissue.
dc.format	Electronic
dc.language	eng
dc.publisher	IOP PUBLISHING LTD
dc.relation	http://purl.org/au-research/grants/arc/DP190101973
dc.relation.ispartof	Biofabrication
dc.relation.isbasedon	10.1088/1758-5090/ac14ca
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0903 Biomedical Engineering, 1004 Medical Biotechnology, 1099 Other Technology
dc.title	Towards engineering heart tissues from bioprinted cardiac spheroids.
dc.type	Journal Article
utslib.citation.volume	13
utslib.location.activity	England
utslib.for	0903 Biomedical Engineering
utslib.for	1004 Medical Biotechnology
utslib.for	1099 Other Technology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	open_access	*
dc.date.updated	2021-11-29T11:02:41Z
pubs.issue	4
pubs.publication-status	Published
pubs.volume	13
utslib.citation.issue	4

Abstract:

Currentin vivoandin vitromodels fail to accurately recapitulate the human heart microenvironment for biomedical applications. This study explores the use of cardiac spheroids (CSs) to biofabricate advancedin vitromodels of the human heart. CSs were created from human cardiac myocytes, fibroblasts and endothelial cells (ECs), mixed within optimal alginate/gelatin hydrogels and then bioprinted on a microelectrode plate for drug testing. Bioprinted CSs maintained their structure and viability for at least 30 d after printing. Vascular endothelial growth factor (VEGF) promoted EC branching from CSs within hydrogels. Alginate/gelatin-based hydrogels enabled spheroids fusion, which was further facilitated by addition of VEGF. Bioprinted CSs contracted spontaneously and under stimulation, allowing to record contractile and electrical signals on the microelectrode plates for industrial applications. Taken together, our findings indicate that bioprinted CSs can be used to biofabricate human heart tissues for long termin vitrotesting. This has the potential to be used to study biochemical, physiological and pharmacological features of human heart tissue.

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http://hdl.handle.net/10453/151913