Controlled synthesis of dicalcium phosphate dihydrate (DCPD) from metastable solutions: insights into pathogenic calcification

Rafeek, AD; Choi, G; Evans, LA

Controlled synthesis of dicalcium phosphate dihydrate (DCPD) from metastable solutions: insights into pathogenic calcification

Rafeek, AD Choi, G Evans, LA

Permalink

Publisher:: Springer Science and Business Media LLC
Publication Type:: Journal Article
Citation:: Journal of Materials Science: Materials in Medicine, 2021, 32, (12)
Issue Date:: 2021-12

Closed Access

	Filename	Description	Size
	Controlled synthesis of dicalcium phosphate dihydrate.pdf	Published version	1 MB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Rafeek, AD
dc.contributor.author	Choi, G
dc.contributor.author	Evans, LA
dc.date.accessioned	2022-01-19T02:27:46Z
dc.date.available	2022-01-19T02:27:46Z
dc.date.issued	2021-12
dc.identifier.citation	Journal of Materials Science: Materials in Medicine, 2021, 32, (12)
dc.identifier.issn	0957-4530
dc.identifier.issn	1573-4838
dc.identifier.uri	http://hdl.handle.net/10453/153345
dc.description.abstract	<jats:title>Abstract</jats:title><jats:p>Calcium phosphate (CaP) compounds may occur in the body as abnormal pathogenic phases in addition to their normal occurrence as bones and teeth. Dicalcium phosphate dihydrate (DCPD; CaPO<jats:sub>4</jats:sub>·2H<jats:sub>2</jats:sub>O), along with other significant CaP phases, have been observed in pathogenic calcifications such as dental calculi, kidney stones and urinary stones. While other studies have shown that polar amino acids can inhibit the growth of CaPs, these studies have mainly focused on hydroxyapatite (HAp; Ca<jats:sub>10</jats:sub>(PO<jats:sub>4</jats:sub>)<jats:sub>6</jats:sub>(OH)<jats:sub>2</jats:sub>) formation from highly supersaturated solutions, while their effects on DCPD nucleation and growth from metastable solutions have been less thoroughly explored. By further elucidating the mechanisms of DCPD formation and the influence of amino acids on those mechanisms, insights may be gained into ways that amino acids could be used in treatment and prevention of unwanted calcifications. The current study involved seeded growth of DCPD from metastable solutions at constant pH in the presence of neutral, acidic and phosphorylated amino acid side chains. As a comparison, solutions were also seeded with calcium pyrophosphate (CPP; Ca<jats:sub>2</jats:sub>P<jats:sub>2</jats:sub>O<jats:sub>7</jats:sub>), a known calcium phosphate inhibitor. The results show that polar amino acids inhibit DCPD growth; this likely occurs due to electrostatic interactions between amino acid side groups and charged DCPD surfaces. Phosphoserine had the greatest inhibitory ability of the amino acids tested, with an effect equal to that of CPP. Clustering of DCPD crystals giving rise to a “chrysanthemum-like” morphology was noted with glutamic acid. This study concludes that molecules containing an increased number of polar side groups will enhance the inhibition of DCPD seeded growth from metastable solutions.</jats:p>
dc.language	en
dc.publisher	Springer Science and Business Media LLC
dc.relation.ispartof	Journal of Materials Science: Materials in Medicine
dc.relation.isbasedon	10.1007/s10856-021-06617-4
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0903 Biomedical Engineering, 0912 Materials Engineering
dc.subject.classification	Biomedical Engineering
dc.title	Controlled synthesis of dicalcium phosphate dihydrate (DCPD) from metastable solutions: insights into pathogenic calcification
dc.type	Journal Article
utslib.citation.volume	32
utslib.for	0903 Biomedical Engineering
utslib.for	0912 Materials Engineering
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access	*
dc.date.updated	2022-01-19T02:27:37Z
pubs.issue	12
pubs.publication-status	Published
pubs.volume	32
utslib.citation.issue	12

Abstract:

AbstractCalcium phosphate (CaP) compounds may occur in the body as abnormal pathogenic phases in addition to their normal occurrence as bones and teeth. Dicalcium phosphate dihydrate (DCPD; CaPO4·2H2O), along with other significant CaP phases, have been observed in pathogenic calcifications such as dental calculi, kidney stones and urinary stones. While other studies have shown that polar amino acids can inhibit the growth of CaPs, these studies have mainly focused on hydroxyapatite (HAp; Ca10(PO4)6(OH)2) formation from highly supersaturated solutions, while their effects on DCPD nucleation and growth from metastable solutions have been less thoroughly explored. By further elucidating the mechanisms of DCPD formation and the influence of amino acids on those mechanisms, insights may be gained into ways that amino acids could be used in treatment and prevention of unwanted calcifications. The current study involved seeded growth of DCPD from metastable solutions at constant pH in the presence of neutral, acidic and phosphorylated amino acid side chains. As a comparison, solutions were also seeded with calcium pyrophosphate (CPP; Ca2P2O7), a known calcium phosphate inhibitor. The results show that polar amino acids inhibit DCPD growth; this likely occurs due to electrostatic interactions between amino acid side groups and charged DCPD surfaces. Phosphoserine had the greatest inhibitory ability of the amino acids tested, with an effect equal to that of CPP. Clustering of DCPD crystals giving rise to a “chrysanthemum-like” morphology was noted with glutamic acid. This study concludes that molecules containing an increased number of polar side groups will enhance the inhibition of DCPD seeded growth from metastable solutions.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/153345