Micromechanical characterisation of 3D bioprinted neural cell models using Brillouin microspectroscopy

Rad, MA; Mahmodi, H; Filipe, EC; Cox, TR; Kabakova, I; Tipper, JL

Micromechanical characterisation of 3D bioprinted neural cell models using Brillouin microspectroscopy

Rad, MA Mahmodi, H Filipe, EC Cox, TR Kabakova, I

Tipper, JL

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Publisher:: Elsevier BV
Publication Type:: Journal Article
Citation:: Bioprinting, 2022, 25, pp. e00179
Issue Date:: 2022-03-01

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Field	Value	Language
dc.contributor.author	Rad, MA
dc.contributor.author	Mahmodi, H
dc.contributor.author	Filipe, EC
dc.contributor.author	Cox, TR
dc.contributor.author	Kabakova, I https://orcid.org/0000-0002-6831-9478
dc.contributor.author	Tipper, JL
dc.date.accessioned	2022-01-27T00:55:01Z
dc.date.available	2022-01-27T00:55:01Z
dc.date.issued	2022-03-01
dc.identifier.citation	Bioprinting, 2022, 25, pp. e00179
dc.identifier.issn	2405-8866
dc.identifier.uri	http://hdl.handle.net/10453/153616
dc.description.abstract	Biofabrication of three-dimensional (3D) in vitro neural cell models that closely mimic the central nervous system (CNS) is an emerging field of research with applications from fundamental biology to regenerative medicine, and far reaching benefits for the economy, healthcare and the ethical use of animals. The micromechanical properties of such models are an important factor dictating the success of modelling outcomes in relation to accurate reproduction of the processes in native tissues. Characterising the micromechanical properties of such models non-destructively and over a prolonged span of time, however, are key challenges. Brillouin microspectroscopy (BM) could provide a solution to this problem since this technology is non-invasive, label-free and is capable of micro-scale 3D imaging. In this work, the micromechanical properties of 3D bioprinted neural cell models consisting of NG 108-15 neuronal cells and Gelatin methacryloyl (GelMA) hydrogels of various concentrations were investigated using BM. We demonstrate changes in the volume-averaged (VA) and local micro-scale mechanical properties of these models over a 7 day period, in which the hydrogel component of the model are found to soften as the cells grow, multiply and form stiffer spheroid-type structures. These findings signify the necessity to resolve in microscopic detail the mechanics of in vitro 3D tissue models and suggest Brillouin microspectroscopy to be a suitable technology to bridge this gap.
dc.language	en
dc.publisher	Elsevier BV
dc.relation.ispartof	Bioprinting
dc.relation.isbasedon	10.1016/j.bprint.2021.e00179
dc.rights	info:eu-repo/semantics/openAccess
dc.title	Micromechanical characterisation of 3D bioprinted neural cell models using Brillouin microspectroscopy
dc.type	Journal Article
utslib.citation.volume	25
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	open_access	*
dc.date.updated	2022-01-27T00:54:59Z
pubs.publication-status	Accepted
pubs.volume	25

Abstract:

Biofabrication of three-dimensional (3D) in vitro neural cell models that closely mimic the central nervous system (CNS) is an emerging field of research with applications from fundamental biology to regenerative medicine, and far reaching benefits for the economy, healthcare and the ethical use of animals. The micromechanical properties of such models are an important factor dictating the success of modelling outcomes in relation to accurate reproduction of the processes in native tissues. Characterising the micromechanical properties of such models non-destructively and over a prolonged span of time, however, are key challenges. Brillouin microspectroscopy (BM) could provide a solution to this problem since this technology is non-invasive, label-free and is capable of micro-scale 3D imaging. In this work, the micromechanical properties of 3D bioprinted neural cell models consisting of NG 108-15 neuronal cells and Gelatin methacryloyl (GelMA) hydrogels of various concentrations were investigated using BM. We demonstrate changes in the volume-averaged (VA) and local micro-scale mechanical properties of these models over a 7 day period, in which the hydrogel component of the model are found to soften as the cells grow, multiply and form stiffer spheroid-type structures. These findings signify the necessity to resolve in microscopic detail the mechanics of in vitro 3D tissue models and suggest Brillouin microspectroscopy to be a suitable technology to bridge this gap.

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http://hdl.handle.net/10453/153616