Effect of protein adsorption on electrospun hemoglobin/gelatin-MWCNTs microbelts modified electrode: Toward electrochemical measurement of hydrogen peroxide

Deng, ZX; Tao, JW; Zhang, W; Mu, HJ; Wu, HJ; Wang, YB; Xu, XX; Zheng, W

Effect of protein adsorption on electrospun hemoglobin/gelatin-MWCNTs microbelts modified electrode: Toward electrochemical measurement of hydrogen peroxide

Deng, ZX Tao, JW Zhang, W Mu, HJ Wu, HJ Wang, YB Xu, XX Zheng, W

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Publisher:: ELSEVIER SCIENCE SA
Publication Type:: Journal Article
Citation:: Materials Chemistry and Physics, 2021, 257
Issue Date:: 2021-01-01

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Field	Value	Language
dc.contributor.author	Deng, ZX
dc.contributor.author	Tao, JW
dc.contributor.author	Zhang, W
dc.contributor.author	Mu, HJ
dc.contributor.author	Wu, HJ
dc.contributor.author	Wang, YB
dc.contributor.author	Xu, XX
dc.contributor.author	Zheng, W
dc.date.accessioned	2022-01-30T03:16:03Z
dc.date.available	2022-01-30T03:16:03Z
dc.date.issued	2021-01-01
dc.identifier.citation	Materials Chemistry and Physics, 2021, 257
dc.identifier.issn	0254-0584
dc.identifier.issn	1879-3312
dc.identifier.uri	http://hdl.handle.net/10453/153841
dc.description.abstract	The on-line electrochemical analysis is one of powerful strategies in analytical chemistry and pathophysiology. To achieve high sensitivity and long-term stability of electrochemical biosensor, the bottleneck challenge is the spontaneous proteins adsorption onto the electrode surface within the biological fluids or in vivo environments. In this work, a hemoglobin/gelatin-multiwalled carbon nanotubes microbelts modified electrode (Hb/gelatin-MWCNTs/GC electrode) was successfully fabricated via one-step electrospinning process. The results of atomic force microscopy (AFM), scanning electron microscopy (SEM) and water contact angle test confirmed the electrospun Hb/gelatin-MWCNTs microbelts possessed smooth and hydrophilic surfaces. Furthermore, the electrospun Hb/gelatin-MWCNTs/GC electrode after protein adsorption displayed an excellent electrocatalytic sensitivity toward the reduction of hydrogen peroxide (H2O2). Moreover, the Hb/gelatin-MWCNTs/GC electrode presented very high biological affinity to H2O2 (Kmapp=503.4 ± 2.8 μmol L−1) after 360 min protein adsorption compared to that of the electrode before protein adsorption (Kmapp=298.1 ± 3.1 μmol L−1). The microbelts constructed H2O2 biosensor showed high selectivity, stability and reproducibility after protein adsorption. Therefore, this work provided the proof of the concept that the electrospun Hb/gelatin-MWCNTs/GC electrode displayed excellent sensing performance to H2O2 after protein adsorption, which could enable the implantable electrochemical biosensor for the on-line analysis.
dc.language	English
dc.publisher	ELSEVIER SCIENCE SA
dc.relation.ispartof	Materials Chemistry and Physics
dc.relation.isbasedon	10.1016/j.matchemphys.2020.123827
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0303 Macromolecular and Materials Chemistry, 0912 Materials Engineering, 1007 Nanotechnology
dc.subject.classification	Materials
dc.title	Effect of protein adsorption on electrospun hemoglobin/gelatin-MWCNTs microbelts modified electrode: Toward electrochemical measurement of hydrogen peroxide
dc.type	Journal Article
utslib.citation.volume	257
utslib.for	0303 Macromolecular and Materials Chemistry
utslib.for	0912 Materials Engineering
utslib.for	1007 Nanotechnology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	closed_access	*
dc.date.updated	2022-01-30T03:15:59Z
pubs.publication-status	Published
pubs.volume	257

Abstract:

The on-line electrochemical analysis is one of powerful strategies in analytical chemistry and pathophysiology. To achieve high sensitivity and long-term stability of electrochemical biosensor, the bottleneck challenge is the spontaneous proteins adsorption onto the electrode surface within the biological fluids or in vivo environments. In this work, a hemoglobin/gelatin-multiwalled carbon nanotubes microbelts modified electrode (Hb/gelatin-MWCNTs/GC electrode) was successfully fabricated via one-step electrospinning process. The results of atomic force microscopy (AFM), scanning electron microscopy (SEM) and water contact angle test confirmed the electrospun Hb/gelatin-MWCNTs microbelts possessed smooth and hydrophilic surfaces. Furthermore, the electrospun Hb/gelatin-MWCNTs/GC electrode after protein adsorption displayed an excellent electrocatalytic sensitivity toward the reduction of hydrogen peroxide (H2O2). Moreover, the Hb/gelatin-MWCNTs/GC electrode presented very high biological affinity to H2O2 (Kmapp=503.4 ± 2.8 μmol L−1) after 360 min protein adsorption compared to that of the electrode before protein adsorption (Kmapp=298.1 ± 3.1 μmol L−1). The microbelts constructed H2O2 biosensor showed high selectivity, stability and reproducibility after protein adsorption. Therefore, this work provided the proof of the concept that the electrospun Hb/gelatin-MWCNTs/GC electrode displayed excellent sensing performance to H2O2 after protein adsorption, which could enable the implantable electrochemical biosensor for the on-line analysis.

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http://hdl.handle.net/10453/153841