Advanced bioengineering of male germ stem cells to preserve fertility.

Eyni, H; Ghorbani, S; Nazari, H; Hajialyani, M; Razavi Bazaz, S; Mohaqiq, M; Ebrahimi Warkiani, M; Sutherland, DS

Advanced bioengineering of male germ stem cells to preserve fertility.

Eyni, H Ghorbani, S Nazari, H Hajialyani, M Razavi Bazaz, S

Mohaqiq, M Ebrahimi Warkiani, M

Sutherland, DS

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Publisher:: SAGE Publications
Publication Type:: Journal Article
Citation:: J Tissue Eng, 2021, 12, pp. 20417314211060590
Issue Date:: 2021-01

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Field	Value	Language
dc.contributor.author	Eyni, H
dc.contributor.author	Ghorbani, S
dc.contributor.author	Nazari, H
dc.contributor.author	Hajialyani, M
dc.contributor.author	Razavi Bazaz, S https://orcid.org/0000-0002-6419-3361
dc.contributor.author	Mohaqiq, M
dc.contributor.author	Ebrahimi Warkiani, M https://orcid.org/0000-0002-4184-1944
dc.contributor.author	Sutherland, DS
dc.date.accessioned	2022-02-22T03:24:17Z
dc.date.available	2021-11-01
dc.date.available	2022-02-22T03:24:17Z
dc.date.issued	2021-01
dc.identifier.citation	J Tissue Eng, 2021, 12, pp. 20417314211060590
dc.identifier.issn	2041-7314
dc.identifier.issn	2041-7314
dc.identifier.uri	http://hdl.handle.net/10453/154768
dc.description.abstract	In modern life, several factors such as genetics, exposure to toxins, and aging have resulted in significant levels of male infertility, estimated to be approximately 18% worldwide. In response, substantial progress has been made to improve in vitro fertilization treatments (e.g. microsurgical testicular sperm extraction (m-TESE), intra-cytoplasmic sperm injection (ICSI), and round spermatid injection (ROSI)). Mimicking the structure of testicular natural extracellular matrices (ECM) outside of the body is one clear route toward complete in vitro spermatogenesis and male fertility preservation. Here, a new wave of technological innovations is underway applying regenerative medicine strategies to cell-tissue culture on natural or synthetic scaffolds supplemented with bioactive factors. The emergence of advanced bioengineered systems suggests new hope for male fertility preservation through development of functional male germ cells. To date, few studies aimed at in vitro spermatogenesis have resulted in relevant numbers of mature gametes. However, a substantial body of knowledge on conditions that are required to maintain and mature male germ cells in vitro is now in place. This review focuses on advanced bioengineering methods such as microfluidic systems, bio-fabricated scaffolds, and 3D organ culture applied to the germline for fertility preservation through in vitro spermatogenesis.
dc.format	Electronic-eCollection
dc.language	eng
dc.publisher	SAGE Publications
dc.relation.ispartof	J Tissue Eng
dc.relation.isbasedon	10.1177/20417314211060590
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	0601 Biochemistry and Cell Biology, 1004 Medical Biotechnology
dc.title	Advanced bioengineering of male germ stem cells to preserve fertility.
dc.type	Journal Article
utslib.citation.volume	12
utslib.location.activity	England
utslib.for	0601 Biochemistry and Cell Biology
utslib.for	1004 Medical Biotechnology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	in_progress	*
dc.date.updated	2022-02-22T03:24:09Z
pubs.publication-status	Published online
pubs.volume	12

Abstract:

In modern life, several factors such as genetics, exposure to toxins, and aging have resulted in significant levels of male infertility, estimated to be approximately 18% worldwide. In response, substantial progress has been made to improve in vitro fertilization treatments (e.g. microsurgical testicular sperm extraction (m-TESE), intra-cytoplasmic sperm injection (ICSI), and round spermatid injection (ROSI)). Mimicking the structure of testicular natural extracellular matrices (ECM) outside of the body is one clear route toward complete in vitro spermatogenesis and male fertility preservation. Here, a new wave of technological innovations is underway applying regenerative medicine strategies to cell-tissue culture on natural or synthetic scaffolds supplemented with bioactive factors. The emergence of advanced bioengineered systems suggests new hope for male fertility preservation through development of functional male germ cells. To date, few studies aimed at in vitro spermatogenesis have resulted in relevant numbers of mature gametes. However, a substantial body of knowledge on conditions that are required to maintain and mature male germ cells in vitro is now in place. This review focuses on advanced bioengineering methods such as microfluidic systems, bio-fabricated scaffolds, and 3D organ culture applied to the germline for fertility preservation through in vitro spermatogenesis.

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http://hdl.handle.net/10453/154768