An easy-to-operate method for single-cell isolation and retrieval using a microfluidic static droplet array.

Ding, L; Radfar, P; Rezaei, M; Warkiani, ME

An easy-to-operate method for single-cell isolation and retrieval using a microfluidic static droplet array.

Ding, L Radfar, P Rezaei, M Warkiani, ME

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Publisher:: Springer
Publication Type:: Journal Article
Citation:: Microchimica Acta: an international journal on micro and trace analysis, 2021, 188, (8), pp. 1-11
Issue Date:: 2021-07-06

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Field	Value	Language
dc.contributor.author	Ding, L
dc.contributor.author	Radfar, P
dc.contributor.author	Rezaei, M
dc.contributor.author	Warkiani, ME
dc.date.accessioned	2022-03-08T04:04:22Z
dc.date.available	2021-06-12
dc.date.available	2022-03-08T04:04:22Z
dc.date.issued	2021-07-06
dc.identifier.citation	Microchimica Acta: an international journal on micro and trace analysis, 2021, 188, (8), pp. 1-11
dc.identifier.issn	0026-3672
dc.identifier.issn	1436-5073
dc.identifier.uri	http://hdl.handle.net/10453/155054
dc.description.abstract	In-depth study of cellular heterogeneity of rare cells (e.g. circulating tumour cells (CTCs) and circulating foetal cells (CFCs)) is greatly needed in disease management but has never been completely explored due to the current technological limitations. We have developed a retrieval method for single-cell detection using a static droplet array (SDA) device through liquid segmentation with almost no sample loss. We explored the potential of using SDA for low sample input and retrieving the cells of interest using everyday laboratory equipment for downstream molecular analysis. This single-cell isolation and retrieval method is low-cost, rapid and provides a solution to the remaining challenge for single rare cell detection. The entire process takes less than 15 min, is easy to fabricate and allows for on-chip analysis of cells in nanolitre droplets and retrieval of desired droplets. To validate the applicability of our device and method, we mimicked detection of single CTCs by isolating and retrieving single cells and perform real-time PCR on their mRNA contents.
dc.format	Electronic
dc.language	eng
dc.publisher	Springer
dc.relation	http://purl.org/au-research/grants/arc/DP170103704
dc.relation	http://purl.org/au-research/grants/arc/DP180103003
dc.relation	http://purl.org/au-research/grants/nhmrc/1143377
dc.relation.ispartof	Microchimica Acta: an international journal on micro and trace analysis
dc.relation.isbasedon	10.1007/s00604-021-04897-9
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0301 Analytical Chemistry, 0399 Other Chemical Sciences
dc.subject.classification	Analytical Chemistry
dc.subject.mesh	Biosensing Techniques
dc.subject.mesh	Cell Separation
dc.subject.mesh	Humans
dc.subject.mesh	Lab-On-A-Chip Devices
dc.subject.mesh	MCF-7 Cells
dc.subject.mesh	Microfluidic Analytical Techniques
dc.subject.mesh	Microfluidics
dc.subject.mesh	Neoplastic Cells, Circulating
dc.subject.mesh	Polymerase Chain Reaction
dc.subject.mesh	Single-Cell Analysis
dc.subject.mesh	THP-1 Cells
dc.subject.mesh	Biosensing Techniques
dc.subject.mesh	Cell Separation
dc.subject.mesh	Humans
dc.subject.mesh	Lab-On-A-Chip Devices
dc.subject.mesh	MCF-7 Cells
dc.subject.mesh	Microfluidic Analytical Techniques
dc.subject.mesh	Microfluidics
dc.subject.mesh	Neoplastic Cells, Circulating
dc.subject.mesh	Polymerase Chain Reaction
dc.subject.mesh	Single-Cell Analysis
dc.subject.mesh	THP-1 Cells
dc.subject.mesh	Humans
dc.subject.mesh	Microfluidic Analytical Techniques
dc.subject.mesh	Cell Separation
dc.subject.mesh	Polymerase Chain Reaction
dc.subject.mesh	Biosensing Techniques
dc.subject.mesh	Microfluidics
dc.subject.mesh	Neoplastic Cells, Circulating
dc.subject.mesh	Lab-On-A-Chip Devices
dc.subject.mesh	Single-Cell Analysis
dc.subject.mesh	MCF-7 Cells
dc.subject.mesh	THP-1 Cells
dc.title	An easy-to-operate method for single-cell isolation and retrieval using a microfluidic static droplet array.
dc.type	Journal Article
utslib.citation.volume	188
utslib.location.activity	Austria
utslib.for	0301 Analytical Chemistry
utslib.for	0399 Other Chemical Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2022-03-08T04:04:18Z
pubs.issue	8
pubs.publication-status	Published
pubs.volume	188
utslib.citation.issue	8

Abstract:

In-depth study of cellular heterogeneity of rare cells (e.g. circulating tumour cells (CTCs) and circulating foetal cells (CFCs)) is greatly needed in disease management but has never been completely explored due to the current technological limitations. We have developed a retrieval method for single-cell detection using a static droplet array (SDA) device through liquid segmentation with almost no sample loss. We explored the potential of using SDA for low sample input and retrieving the cells of interest using everyday laboratory equipment for downstream molecular analysis. This single-cell isolation and retrieval method is low-cost, rapid and provides a solution to the remaining challenge for single rare cell detection. The entire process takes less than 15 min, is easy to fabricate and allows for on-chip analysis of cells in nanolitre droplets and retrieval of desired droplets. To validate the applicability of our device and method, we mimicked detection of single CTCs by isolating and retrieving single cells and perform real-time PCR on their mRNA contents.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/155054