A comparative analysis of single cell small RNA sequencing data reveals heterogeneous isomiR expression and regulation.

Smith, CM; Hutvagner, G

A comparative analysis of single cell small RNA sequencing data reveals heterogeneous isomiR expression and regulation.

Smith, CM Hutvagner, G

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Publisher:: Springer Science and Business Media LLC
Publication Type:: Journal Article
Citation:: Sci Rep, 2022, 12, (1), pp. 2834
Issue Date:: 2022-02-18

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Field	Value	Language
dc.contributor.author	Smith, CM
dc.contributor.author	Hutvagner, G https://orcid.org/0000-0002-7231-9446
dc.date.accessioned	2022-04-07T06:52:56Z
dc.date.available	2022-01-31
dc.date.available	2022-04-07T06:52:56Z
dc.date.issued	2022-02-18
dc.identifier.citation	Sci Rep, 2022, 12, (1), pp. 2834
dc.identifier.issn	2045-2322
dc.identifier.issn	2045-2322
dc.identifier.uri	http://hdl.handle.net/10453/155980
dc.description.abstract	MicroRNAs (miRNAs) are non-coding small RNAs which play a critical role in the regulation of gene expression in cells. It is known that miRNAs are often expressed as multiple isoforms, called isomiRs, which may have alternative regulatory functions. Despite the recent development of several single cell small RNA sequencing protocols, these methods have not been leveraged to investigate isomiR expression and regulation to better understand their role on a single cell level. Here we integrate sequencing data from three independent studies and find substantial differences in isomiR composition that suggest that cell autonomous mechanisms may drive isomiR processing. We also find evidence of altered regulatory functions of different classes of isomiRs, when compared to their respective wild-type miRNA, which supports a biological role for many of the isomiRs that are expressed.
dc.format	Electronic
dc.language	eng
dc.publisher	Springer Science and Business Media LLC
dc.relation.ispartof	Sci Rep
dc.relation.isbasedon	10.1038/s41598-022-06876-3
dc.rights	info:eu-repo/semantics/openAccess
dc.subject.mesh	Gene Expression Regulation
dc.subject.mesh	Humans
dc.subject.mesh	MicroRNAs
dc.subject.mesh	RNA Isoforms
dc.subject.mesh	RNA-Seq
dc.subject.mesh	Sequence Analysis, RNA
dc.subject.mesh	Single-Cell Analysis
dc.subject.mesh	Whole Exome Sequencing
dc.subject.mesh	Humans
dc.subject.mesh	MicroRNAs
dc.subject.mesh	Sequence Analysis, RNA
dc.subject.mesh	Gene Expression Regulation
dc.subject.mesh	Single-Cell Analysis
dc.subject.mesh	RNA Isoforms
dc.subject.mesh	Whole Exome Sequencing
dc.subject.mesh	RNA-Seq
dc.title	A comparative analysis of single cell small RNA sequencing data reveals heterogeneous isomiR expression and regulation.
dc.type	Journal Article
utslib.citation.volume	12
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	open_access	*
dc.date.updated	2022-04-07T06:52:50Z
pubs.issue	1
pubs.publication-status	Published online
pubs.volume	12
utslib.citation.issue	1

Abstract:

MicroRNAs (miRNAs) are non-coding small RNAs which play a critical role in the regulation of gene expression in cells. It is known that miRNAs are often expressed as multiple isoforms, called isomiRs, which may have alternative regulatory functions. Despite the recent development of several single cell small RNA sequencing protocols, these methods have not been leveraged to investigate isomiR expression and regulation to better understand their role on a single cell level. Here we integrate sequencing data from three independent studies and find substantial differences in isomiR composition that suggest that cell autonomous mechanisms may drive isomiR processing. We also find evidence of altered regulatory functions of different classes of isomiRs, when compared to their respective wild-type miRNA, which supports a biological role for many of the isomiRs that are expressed.

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http://hdl.handle.net/10453/155980