Tumor dissociation of highly viable cell suspensions for single-cell omic analyses in mouse models of breast cancer.

Rodriguez de la Fuente, L; Law, AMK; Gallego-Ortega, D; Valdes-Mora, F

Tumor dissociation of highly viable cell suspensions for single-cell omic analyses in mouse models of breast cancer.

Rodriguez de la Fuente, L Law, AMK Gallego-Ortega, D Valdes-Mora, F

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Publisher:: Elsevier BV
Publication Type:: Journal Article
Citation:: STAR Protoc, 2021, 2, (4), pp. 100841
Issue Date:: 2021-12-17

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Field	Value	Language
dc.contributor.author	Rodriguez de la Fuente, L
dc.contributor.author	Law, AMK
dc.contributor.author	Gallego-Ortega, D
dc.contributor.author	Valdes-Mora, F
dc.date.accessioned	2022-04-13T12:15:40Z
dc.date.available	2022-04-13T12:15:40Z
dc.date.issued	2021-12-17
dc.identifier.citation	STAR Protoc, 2021, 2, (4), pp. 100841
dc.identifier.issn	2666-1667
dc.identifier.issn	2666-1667
dc.identifier.uri	http://hdl.handle.net/10453/156210
dc.description.abstract	Cell preparation with a high rate of viable cells is required to obtain reliable single-cell transcriptomic and epigenomic data. This protocol describes a technique for digestion and single-cell isolation from mouse mammary tumors to achieve ∼90% of viable cells, which can be subsequently processed in a diverse array of high-throughput single-cell "omic platforms," both in an unbiased manner or after selection of a specific cell population. For complete details on the use and execution of this protocol, please refer to Valdes-Mora et al. (2021).
dc.format	Electronic-eCollection
dc.language	eng
dc.publisher	Elsevier BV
dc.relation.ispartof	STAR Protoc
dc.relation.isbasedon	10.1016/j.xpro.2021.100841
dc.rights	info:eu-repo/semantics/openAccess
dc.title	Tumor dissociation of highly viable cell suspensions for single-cell omic analyses in mouse models of breast cancer.
dc.type	Journal Article
utslib.citation.volume	2
utslib.location.activity	United States
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	open_access	*
dc.date.updated	2022-04-13T12:15:33Z
pubs.issue	4
pubs.publication-status	Published online
pubs.volume	2
utslib.citation.issue	4

Abstract:

Cell preparation with a high rate of viable cells is required to obtain reliable single-cell transcriptomic and epigenomic data. This protocol describes a technique for digestion and single-cell isolation from mouse mammary tumors to achieve ∼90% of viable cells, which can be subsequently processed in a diverse array of high-throughput single-cell "omic platforms," both in an unbiased manner or after selection of a specific cell population. For complete details on the use and execution of this protocol, please refer to Valdes-Mora et al. (2021).

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/156210