A RhoA-FRET Biosensor Mouse for Intravital Imaging in Normal Tissue Homeostasis and Disease Contexts.

Nobis, M; Herrmann, D; Warren, SC; Kadir, S; Leung, W; Killen, M; Magenau, A; Stevenson, D; Lucas, MC; Reischmann, N; Vennin, C; Conway, JRW; Boulghourjian, A; Zaratzian, A; Law, AM; Gallego-Ortega, D; Ormandy, CJ; Walters, SN; Grey, ST; Bailey, J; Chtanova, T; Quinn, JMW; Baldock, PA; Croucher, PI; Schwarz, JP; Mrowinska, A; Zhang, L; Herzog, H; Masedunskas, A; Hardeman, EC; Gunning, PW; Del Monte-Nieto, G; Harvey, RP; Samuel, MS; Pajic, M; McGhee, EJ; Johnsson, A-KE; Sansom, OJ; Welch, HCE; Morton, JP; Strathdee, D; Anderson, KI; Timpson, P

A RhoA-FRET Biosensor Mouse for Intravital Imaging in Normal Tissue Homeostasis and Disease Contexts.

Nobis, M Herrmann, D Warren, SC Kadir, S Leung, W Killen, M Magenau, A Stevenson, D Lucas, MC Reischmann, N Vennin, C Conway, JRW Boulghourjian, A Zaratzian, A Law, AM Gallego-Ortega, D Ormandy, CJ Walters, SN Grey, ST Bailey, J Chtanova, T Quinn, JMW Baldock, PA Croucher, PI Schwarz, JP Mrowinska, A Zhang, L Herzog, H Masedunskas, A Hardeman, EC Gunning, PW Del Monte-Nieto, G Harvey, RP Samuel, MS Pajic, M McGhee, EJ Johnsson, A-KE Sansom, OJ Welch, HCE Morton, JP Strathdee, D Anderson, KI Timpson, P

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Publisher:: CELL PRESS
Publication Type:: Journal Article
Citation:: Cell Rep, 2017, 21, (1), pp. 274-288
Issue Date:: 2017-10-03

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Field	Value	Language
dc.contributor.author	Nobis, M
dc.contributor.author	Herrmann, D
dc.contributor.author	Warren, SC
dc.contributor.author	Kadir, S
dc.contributor.author	Leung, W
dc.contributor.author	Killen, M
dc.contributor.author	Magenau, A
dc.contributor.author	Stevenson, D
dc.contributor.author	Lucas, MC
dc.contributor.author	Reischmann, N
dc.contributor.author	Vennin, C
dc.contributor.author	Conway, JRW
dc.contributor.author	Boulghourjian, A
dc.contributor.author	Zaratzian, A
dc.contributor.author	Law, AM
dc.contributor.author	Gallego-Ortega, D
dc.contributor.author	Ormandy, CJ
dc.contributor.author	Walters, SN
dc.contributor.author	Grey, ST
dc.contributor.author	Bailey, J
dc.contributor.author	Chtanova, T
dc.contributor.author	Quinn, JMW
dc.contributor.author	Baldock, PA
dc.contributor.author	Croucher, PI
dc.contributor.author	Schwarz, JP
dc.contributor.author	Mrowinska, A
dc.contributor.author	Zhang, L
dc.contributor.author	Herzog, H
dc.contributor.author	Masedunskas, A
dc.contributor.author	Hardeman, EC
dc.contributor.author	Gunning, PW
dc.contributor.author	Del Monte-Nieto, G
dc.contributor.author	Harvey, RP
dc.contributor.author	Samuel, MS
dc.contributor.author	Pajic, M
dc.contributor.author	McGhee, EJ
dc.contributor.author	Johnsson, A-KE
dc.contributor.author	Sansom, OJ
dc.contributor.author	Welch, HCE
dc.contributor.author	Morton, JP
dc.contributor.author	Strathdee, D
dc.contributor.author	Anderson, KI
dc.contributor.author	Timpson, P
dc.date.accessioned	2022-04-13T12:42:57Z
dc.date.available	2017-09-05
dc.date.available	2022-04-13T12:42:57Z
dc.date.issued	2017-10-03
dc.identifier.citation	Cell Rep, 2017, 21, (1), pp. 274-288
dc.identifier.issn	2211-1247
dc.identifier.issn	2211-1247
dc.identifier.uri	http://hdl.handle.net/10453/156228
dc.description.abstract	The small GTPase RhoA is involved in a variety of fundamental processes in normal tissue. Spatiotemporal control of RhoA is thought to govern mechanosensing, growth, and motility of cells, while its deregulation is associated with disease development. Here, we describe the generation of a RhoA-fluorescence resonance energy transfer (FRET) biosensor mouse and its utility for monitoring real-time activity of RhoA in a variety of native tissues in vivo. We assess changes in RhoA activity during mechanosensing of osteocytes within the bone and during neutrophil migration. We also demonstrate spatiotemporal order of RhoA activity within crypt cells of the small intestine and during different stages of mammary gestation. Subsequently, we reveal co-option of RhoA activity in both invasive breast and pancreatic cancers, and we assess drug targeting in these disease settings, illustrating the potential for utilizing this mouse to study RhoA activity in vivo in real time.
dc.format	Print
dc.language	eng
dc.publisher	CELL PRESS
dc.relation.ispartof	Cell Rep
dc.relation.isbasedon	10.1016/j.celrep.2017.09.022
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0601 Biochemistry and Cell Biology, 1116 Medical Physiology
dc.subject.mesh	Animals
dc.subject.mesh	Antineoplastic Agents
dc.subject.mesh	Biosensing Techniques
dc.subject.mesh	Bone and Bones
dc.subject.mesh	Cell Movement
dc.subject.mesh	Dasatinib
dc.subject.mesh	Erlotinib Hydrochloride
dc.subject.mesh	Female
dc.subject.mesh	Fluorescence Resonance Energy Transfer
dc.subject.mesh	Gene Expression Regulation
dc.subject.mesh	Intestine, Small
dc.subject.mesh	Intravital Microscopy
dc.subject.mesh	Mammary Glands, Animal
dc.subject.mesh	Mammary Neoplasms, Experimental
dc.subject.mesh	Mechanotransduction, Cellular
dc.subject.mesh	Mice
dc.subject.mesh	Mice, Transgenic
dc.subject.mesh	Neutrophils
dc.subject.mesh	Osteocytes
dc.subject.mesh	Pancreatic Neoplasms
dc.subject.mesh	Time-Lapse Imaging
dc.subject.mesh	rho GTP-Binding Proteins
dc.subject.mesh	rhoA GTP-Binding Protein
dc.subject.mesh	Bone and Bones
dc.subject.mesh	Intestine, Small
dc.subject.mesh	Mammary Glands, Animal
dc.subject.mesh	Neutrophils
dc.subject.mesh	Osteocytes
dc.subject.mesh	Animals
dc.subject.mesh	Mice, Transgenic
dc.subject.mesh	Mice
dc.subject.mesh	Pancreatic Neoplasms
dc.subject.mesh	Mammary Neoplasms, Experimental
dc.subject.mesh	rho GTP-Binding Proteins
dc.subject.mesh	rhoA GTP-Binding Protein
dc.subject.mesh	Antineoplastic Agents
dc.subject.mesh	Fluorescence Resonance Energy Transfer
dc.subject.mesh	Biosensing Techniques
dc.subject.mesh	Mechanotransduction, Cellular
dc.subject.mesh	Cell Movement
dc.subject.mesh	Gene Expression Regulation
dc.subject.mesh	Female
dc.subject.mesh	Time-Lapse Imaging
dc.subject.mesh	Intravital Microscopy
dc.subject.mesh	Dasatinib
dc.subject.mesh	Erlotinib Hydrochloride
dc.title	A RhoA-FRET Biosensor Mouse for Intravital Imaging in Normal Tissue Homeostasis and Disease Contexts.
dc.type	Journal Article
utslib.citation.volume	21
utslib.location.activity	United States
utslib.for	0601 Biochemistry and Cell Biology
utslib.for	1116 Medical Physiology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	open_access	*
dc.date.updated	2022-04-13T12:42:49Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	21
utslib.citation.issue	1

Abstract:

The small GTPase RhoA is involved in a variety of fundamental processes in normal tissue. Spatiotemporal control of RhoA is thought to govern mechanosensing, growth, and motility of cells, while its deregulation is associated with disease development. Here, we describe the generation of a RhoA-fluorescence resonance energy transfer (FRET) biosensor mouse and its utility for monitoring real-time activity of RhoA in a variety of native tissues in vivo. We assess changes in RhoA activity during mechanosensing of osteocytes within the bone and during neutrophil migration. We also demonstrate spatiotemporal order of RhoA activity within crypt cells of the small intestine and during different stages of mammary gestation. Subsequently, we reveal co-option of RhoA activity in both invasive breast and pancreatic cancers, and we assess drug targeting in these disease settings, illustrating the potential for utilizing this mouse to study RhoA activity in vivo in real time.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/156228