Singlet oxygen damages the function of Photosystem II in isolated thylakoids and in the green alga Chlorella sorokiniana

Bashir, F; Rehman, AU; Szabó, M; Vass, I

Singlet oxygen damages the function of Photosystem II in isolated thylakoids and in the green alga Chlorella sorokiniana

Bashir, F Rehman, AU Szabó, M Vass, I

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Publisher:: Springer
Publication Type:: Journal Article
Citation:: Photosynthesis Research, 2021, 149, (1-2), pp. 93-105
Issue Date:: 2021-05-19

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Field	Value	Language
dc.contributor.author	Bashir, F
dc.contributor.author	Rehman, AU
dc.contributor.author	Szabó, M
dc.contributor.author	Vass, I
dc.date.accessioned	2022-05-02T02:03:59Z
dc.date.available	2021-04-26
dc.date.available	2022-05-02T02:03:59Z
dc.date.issued	2021-05-19
dc.identifier.citation	Photosynthesis Research, 2021, 149, (1-2), pp. 93-105
dc.identifier.issn	0166-8595
dc.identifier.issn	1573-5079
dc.identifier.uri	http://hdl.handle.net/10453/156904
dc.description.abstract	Singlet oxygen (1O2) is an important damaging agent, which is produced during illumination by the interaction of the triplet excited state pigment molecules with molecular oxygen. In cells of photosynthetic organisms 1O2 is formed primarily in chlorophyll containing complexes, and damages pigments, lipids, proteins and other cellular constituents in their environment. A useful approach to study the physiological role of 1O2 is the utilization of external photosensitizers. In the present study, we employed a multiwell plate-based screening method in combination with chlorophyll fluorescence imaging to characterize the effect of externally produced 1O2 on the photosynthetic activity of isolated thylakoid membranes and intact Chlorella sorokiniana cells. The results show that the external 1O2 produced by the photosensitization reactions of Rose Bengal damages Photosystem II both in isolated thylakoid membranes and in intact cells in a concentration dependent manner indicating that 1O2 plays a significant role in photodamage of Photosystem II.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Springer
dc.relation.ispartof	Photosynthesis Research
dc.relation.isbasedon	10.1007/s11120-021-00841-3
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0601 Biochemistry and Cell Biology, 0604 Genetics, 0607 Plant Biology
dc.subject.classification	Plant Biology & Botany
dc.subject.mesh	Chlorella
dc.subject.mesh	Photosystem II Protein Complex
dc.subject.mesh	Singlet Oxygen
dc.subject.mesh	Spinacia oleracea
dc.subject.mesh	Thylakoids
dc.subject.mesh	Thylakoids
dc.subject.mesh	Chlorella
dc.subject.mesh	Spinacia oleracea
dc.subject.mesh	Singlet Oxygen
dc.subject.mesh	Photosystem II Protein Complex
dc.title	Singlet oxygen damages the function of Photosystem II in isolated thylakoids and in the green alga Chlorella sorokiniana
dc.type	Journal Article
utslib.citation.volume	149
utslib.location.activity	Netherlands
utslib.for	0601 Biochemistry and Cell Biology
utslib.for	0604 Genetics
utslib.for	0607 Plant Biology
utslib.copyright.status	open_access	*
pubs.consider-herdc	false
dc.date.updated	2022-05-02T02:03:51Z
pubs.issue	1-2
pubs.publication-status	Published
pubs.volume	149
utslib.citation.issue	1-2

Abstract:

Singlet oxygen (1O2) is an important damaging agent, which is produced during illumination by the interaction of the triplet excited state pigment molecules with molecular oxygen. In cells of photosynthetic organisms 1O2 is formed primarily in chlorophyll containing complexes, and damages pigments, lipids, proteins and other cellular constituents in their environment. A useful approach to study the physiological role of 1O2 is the utilization of external photosensitizers. In the present study, we employed a multiwell plate-based screening method in combination with chlorophyll fluorescence imaging to characterize the effect of externally produced 1O2 on the photosynthetic activity of isolated thylakoid membranes and intact Chlorella sorokiniana cells. The results show that the external 1O2 produced by the photosensitization reactions of Rose Bengal damages Photosystem II both in isolated thylakoid membranes and in intact cells in a concentration dependent manner indicating that 1O2 plays a significant role in photodamage of Photosystem II.

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http://hdl.handle.net/10453/156904