A rapid co-culture stamping device for studying intercellular communication.
Hassanzadeh-Barforoushi, A
Shemesh, J
Farbehi, N
Asadnia, M
Yeoh, GH
Harvey, RP
Nordon, RE
Warkiani, ME
- Publisher:
- NATURE PUBLISHING GROUP
- Publication Type:
- Journal Article
- Citation:
- Sci Rep, 2016, 6, (1), pp. 35618
- Issue Date:
- 2016-10-18
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Full metadata record
Field | Value | Language |
---|---|---|
dc.contributor.author | Hassanzadeh-Barforoushi, A | |
dc.contributor.author | Shemesh, J | |
dc.contributor.author | Farbehi, N | |
dc.contributor.author | Asadnia, M | |
dc.contributor.author | Yeoh, GH | |
dc.contributor.author | Harvey, RP | |
dc.contributor.author | Nordon, RE | |
dc.contributor.author | Warkiani, ME | |
dc.date.accessioned | 2022-07-12T23:02:59Z | |
dc.date.available | 2016-09-26 | |
dc.date.available | 2022-07-12T23:02:59Z | |
dc.date.issued | 2016-10-18 | |
dc.identifier.citation | Sci Rep, 2016, 6, (1), pp. 35618 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | http://hdl.handle.net/10453/158816 | |
dc.description.abstract | Regulation of tissue development and repair depends on communication between neighbouring cells. Recent advances in cell micro-contact printing and microfluidics have facilitated the in-vitro study of homotypic and heterotypic cell-cell interaction. Nonetheless, these techniques are still complicated to perform and as a result, are seldom used by biologists. We report here development of a temporarily sealed microfluidic stamping device which utilizes a novel valve design for patterning two adherent cell lines with well-defined interlacing configurations to study cell-cell interactions. We demonstrate post-stamping cell viability of >95%, the stamping of multiple adherent cell types, and the ability to control the seeded cell density. We also show viability, proliferation and migration of cultured cells, enabling analysis of co-culture boundary conditions on cell fate. We also developed an in-vitro model of endothelial and cardiac stem cell interactions, which are thought to regulate coronary repair after myocardial injury. The stamp is fabricated using microfabrication techniques, is operated with a lab pipettor and uses very low reagent volumes of 20 μl with cell injection efficiency of >70%. This easy-to-use device provides a general strategy for micro-patterning of multiple cell types and will be important for studying cell-cell interactions in a multitude of applications. | |
dc.format | Electronic | |
dc.language | eng | |
dc.publisher | NATURE PUBLISHING GROUP | |
dc.relation.ispartof | Sci Rep | |
dc.relation.isbasedon | 10.1038/srep35618 | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject.mesh | Animals | |
dc.subject.mesh | Cell Adhesion | |
dc.subject.mesh | Cell Communication | |
dc.subject.mesh | Cell Line | |
dc.subject.mesh | Cell Movement | |
dc.subject.mesh | Cell Proliferation | |
dc.subject.mesh | Cell Survival | |
dc.subject.mesh | Coculture Techniques | |
dc.subject.mesh | Endothelial Cells | |
dc.subject.mesh | Lab-On-A-Chip Devices | |
dc.subject.mesh | Mice | |
dc.subject.mesh | Microfluidics | |
dc.subject.mesh | Microtechnology | |
dc.subject.mesh | Myocytes, Cardiac | |
dc.subject.mesh | Wound Healing | |
dc.subject.mesh | Cell Line | |
dc.subject.mesh | Endothelial Cells | |
dc.subject.mesh | Myocytes, Cardiac | |
dc.subject.mesh | Animals | |
dc.subject.mesh | Mice | |
dc.subject.mesh | Coculture Techniques | |
dc.subject.mesh | Microfluidics | |
dc.subject.mesh | Wound Healing | |
dc.subject.mesh | Cell Adhesion | |
dc.subject.mesh | Cell Communication | |
dc.subject.mesh | Cell Proliferation | |
dc.subject.mesh | Cell Movement | |
dc.subject.mesh | Cell Survival | |
dc.subject.mesh | Microtechnology | |
dc.subject.mesh | Lab-On-A-Chip Devices | |
dc.title | A rapid co-culture stamping device for studying intercellular communication. | |
dc.type | Journal Article | |
utslib.citation.volume | 6 | |
utslib.location.activity | England | |
pubs.organisational-group | /University of Technology Sydney | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Engineering and Information Technology | |
pubs.organisational-group | /University of Technology Sydney/Strength - CHT - Health Technologies | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering | |
pubs.organisational-group | /University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices | |
pubs.organisational-group | /University of Technology Sydney/Centre for Health Technologies (CHT) | |
utslib.copyright.status | open_access | * |
dc.date.updated | 2022-07-12T23:02:54Z | |
pubs.issue | 1 | |
pubs.publication-status | Published online | |
pubs.volume | 6 | |
utslib.citation.issue | 1 |
Abstract:
Regulation of tissue development and repair depends on communication between neighbouring cells. Recent advances in cell micro-contact printing and microfluidics have facilitated the in-vitro study of homotypic and heterotypic cell-cell interaction. Nonetheless, these techniques are still complicated to perform and as a result, are seldom used by biologists. We report here development of a temporarily sealed microfluidic stamping device which utilizes a novel valve design for patterning two adherent cell lines with well-defined interlacing configurations to study cell-cell interactions. We demonstrate post-stamping cell viability of >95%, the stamping of multiple adherent cell types, and the ability to control the seeded cell density. We also show viability, proliferation and migration of cultured cells, enabling analysis of co-culture boundary conditions on cell fate. We also developed an in-vitro model of endothelial and cardiac stem cell interactions, which are thought to regulate coronary repair after myocardial injury. The stamp is fabricated using microfabrication techniques, is operated with a lab pipettor and uses very low reagent volumes of 20 μl with cell injection efficiency of >70%. This easy-to-use device provides a general strategy for micro-patterning of multiple cell types and will be important for studying cell-cell interactions in a multitude of applications.
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