Short term ex-vivo expansion of circulating head and neck tumour cells.

Kulasinghe, A; Perry, C; Warkiani, ME; Blick, T; Davies, A; O'Byrne, K; Thompson, EW; Nelson, CC; Vela, I; Punyadeera, C

Short term ex-vivo expansion of circulating head and neck tumour cells.

Kulasinghe, A Perry, C Warkiani, ME Blick, T Davies, A O'Byrne, K Thompson, EW Nelson, CC Vela, I Punyadeera, C

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Publisher:: IMPACT JOURNALS LLC
Publication Type:: Journal Article
Citation:: Oncotarget, 2016, 7, (37), pp. 60101-60109
Issue Date:: 2016-09-13

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Field	Value	Language
dc.contributor.author	Kulasinghe, A
dc.contributor.author	Perry, C
dc.contributor.author	Warkiani, ME
dc.contributor.author	Blick, T
dc.contributor.author	Davies, A
dc.contributor.author	O'Byrne, K
dc.contributor.author	Thompson, EW
dc.contributor.author	Nelson, CC
dc.contributor.author	Vela, I
dc.contributor.author	Punyadeera, C
dc.date.accessioned	2022-07-12T23:15:44Z
dc.date.available	2016-07-20
dc.date.available	2022-07-12T23:15:44Z
dc.date.issued	2016-09-13
dc.identifier.citation	Oncotarget, 2016, 7, (37), pp. 60101-60109
dc.identifier.issn	1949-2553
dc.identifier.issn	1949-2553
dc.identifier.uri	http://hdl.handle.net/10453/158817
dc.description.abstract	Minimally invasive techniques are required for the identification of head and neck cancer (HNC) patients who are at an increased risk of metastasis, or are not responding to therapy. An approach utilised in other solid cancers is the identification and enumeration of circulating tumour cells (CTCs) in the peripheral blood of patients. Low numbers of CTCs has been a limiting factor in the HNC field to date. Here we present a methodology to expand HNC patient derived CTCs ex-vivo. As a proof of principle study, 25 advanced stage HNC patient bloods were enriched for circulating tumour cells through negative selection and cultured in 2D and 3D culture environments under hypoxic conditions (2% O2, 5% CO2). CTCs were detected in 14/25 (56%) of patients (ranging from 1-15 CTCs/5 mL blood). Short term CTC cultures were successfully generated in 7/25 advanced stage HNC patients (5/7 of these cultures were from HPV+ patients). Blood samples from which CTC culture was successful had higher CTC counts (p = 0.0002), and were predominantly from HPV+ patients (p = 0.007). This is, to our knowledge, the first pilot study to culture HNC CTCs ex-vivo. Further studies are warranted to determine the use of short term expansion in HNC and the role of HPV in promoting culture success.
dc.format	Print
dc.language	eng
dc.publisher	IMPACT JOURNALS LLC
dc.relation.ispartof	Oncotarget
dc.relation.isbasedon	10.18632/oncotarget.11159
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	1112 Oncology and Carcinogenesis
dc.subject.mesh	Aged
dc.subject.mesh	Aged, 80 and over
dc.subject.mesh	Cell Count
dc.subject.mesh	Cell Culture Techniques
dc.subject.mesh	Cell Proliferation
dc.subject.mesh	ErbB Receptors
dc.subject.mesh	Female
dc.subject.mesh	Head and Neck Neoplasms
dc.subject.mesh	Humans
dc.subject.mesh	In Situ Hybridization, Fluorescence
dc.subject.mesh	Male
dc.subject.mesh	Middle Aged
dc.subject.mesh	Neoplastic Cells, Circulating
dc.subject.mesh	Pilot Projects
dc.subject.mesh	Time Factors
dc.subject.mesh	Tumor Cells, Cultured
dc.subject.mesh	Tumor Cells, Cultured
dc.subject.mesh	Humans
dc.subject.mesh	Head and Neck Neoplasms
dc.subject.mesh	Cell Culture Techniques
dc.subject.mesh	Cell Count
dc.subject.mesh	In Situ Hybridization, Fluorescence
dc.subject.mesh	Pilot Projects
dc.subject.mesh	Cell Proliferation
dc.subject.mesh	Time Factors
dc.subject.mesh	Aged
dc.subject.mesh	Aged, 80 and over
dc.subject.mesh	Middle Aged
dc.subject.mesh	Female
dc.subject.mesh	Male
dc.subject.mesh	Neoplastic Cells, Circulating
dc.subject.mesh	ErbB Receptors
dc.title	Short term ex-vivo expansion of circulating head and neck tumour cells.
dc.type	Journal Article
utslib.citation.volume	7
utslib.location.activity	United States
utslib.for	1112 Oncology and Carcinogenesis
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	open_access	*
dc.date.updated	2022-07-12T23:15:38Z
pubs.issue	37
pubs.publication-status	Published
pubs.volume	7
utslib.citation.issue	37

Abstract:

Minimally invasive techniques are required for the identification of head and neck cancer (HNC) patients who are at an increased risk of metastasis, or are not responding to therapy. An approach utilised in other solid cancers is the identification and enumeration of circulating tumour cells (CTCs) in the peripheral blood of patients. Low numbers of CTCs has been a limiting factor in the HNC field to date. Here we present a methodology to expand HNC patient derived CTCs ex-vivo. As a proof of principle study, 25 advanced stage HNC patient bloods were enriched for circulating tumour cells through negative selection and cultured in 2D and 3D culture environments under hypoxic conditions (2% O2, 5% CO2). CTCs were detected in 14/25 (56%) of patients (ranging from 1-15 CTCs/5 mL blood). Short term CTC cultures were successfully generated in 7/25 advanced stage HNC patients (5/7 of these cultures were from HPV+ patients). Blood samples from which CTC culture was successful had higher CTC counts (p = 0.0002), and were predominantly from HPV+ patients (p = 0.007). This is, to our knowledge, the first pilot study to culture HNC CTCs ex-vivo. Further studies are warranted to determine the use of short term expansion in HNC and the role of HPV in promoting culture success.

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http://hdl.handle.net/10453/158817