Engineering a 3D microfluidic culture platform for tumor-treating field application.

Pavesi, A; Adriani, G; Tay, A; Warkiani, ME; Yeap, WH; Wong, SC; Kamm, RD

Engineering a 3D microfluidic culture platform for tumor-treating field application.

Pavesi, A Adriani, G Tay, A Warkiani, ME Yeap, WH Wong, SC Kamm, RD

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Publisher:: NATURE PUBLISHING GROUP
Publication Type:: Journal Article
Citation:: Sci Rep, 2016, 6, (1), pp. 26584
Issue Date:: 2016-05-24

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Field	Value	Language
dc.contributor.author	Pavesi, A
dc.contributor.author	Adriani, G
dc.contributor.author	Tay, A
dc.contributor.author	Warkiani, ME
dc.contributor.author	Yeap, WH
dc.contributor.author	Wong, SC
dc.contributor.author	Kamm, RD
dc.date.accessioned	2022-07-13T05:49:59Z
dc.date.available	2016-05-04
dc.date.available	2022-07-13T05:49:59Z
dc.date.issued	2016-05-24
dc.identifier.citation	Sci Rep, 2016, 6, (1), pp. 26584
dc.identifier.issn	2045-2322
dc.identifier.issn	2045-2322
dc.identifier.uri	http://hdl.handle.net/10453/158853
dc.description.abstract	The limitations of current cancer therapies highlight the urgent need for a more effective therapeutic strategy. One promising approach uses an alternating electric field; however, the mechanisms involved in the disruption of the cancer cell cycle as well as the potential adverse effects on non-cancerous cells must be clarified. In this study, we present a novel microfluidic device with embedded electrodes that enables the application of an alternating electric field therapy to cancer cells in a 3D extracellular matrix. To demonstrate the potential of our system to aid in designing and testing new therapeutic approaches, cancer cells and cancer cell aggregates were cultured individually or co-cultured with endothelial cells. The metastatic potential of the cancer cells was reduced after electric field treatment. Moreover, the proliferation rate of the treated cancer cells was lower compared with that of the untreated cells, whereas the morphologies and proliferative capacities of the endothelial cells were not significantly affected. These results demonstrate that our novel system can be used to rapidly screen the effect of an alternating electric field on cancer and normal cells within an in vivo-like microenvironment with the potential to optimize treatment protocols and evaluate synergies between tumor-treating field treatment and chemotherapy.
dc.format	Electronic
dc.language	eng
dc.publisher	NATURE PUBLISHING GROUP
dc.relation.ispartof	Sci Rep
dc.relation.isbasedon	10.1038/srep26584
dc.rights	info:eu-repo/semantics/openAccess
dc.rights	This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0
dc.subject.mesh	A549 Cells
dc.subject.mesh	Cell Line, Tumor
dc.subject.mesh	Cell Proliferation
dc.subject.mesh	Cell Survival
dc.subject.mesh	Coculture Techniques
dc.subject.mesh	Electric Stimulation Therapy
dc.subject.mesh	Endothelial Cells
dc.subject.mesh	Equipment Design
dc.subject.mesh	Gene Expression Regulation, Neoplastic
dc.subject.mesh	Human Umbilical Vein Endothelial Cells
dc.subject.mesh	Humans
dc.subject.mesh	Microfluidic Analytical Techniques
dc.subject.mesh	Neoplasms
dc.subject.mesh	Cell Line, Tumor
dc.subject.mesh	Endothelial Cells
dc.subject.mesh	Humans
dc.subject.mesh	Neoplasms
dc.subject.mesh	Electric Stimulation Therapy
dc.subject.mesh	Microfluidic Analytical Techniques
dc.subject.mesh	Coculture Techniques
dc.subject.mesh	Equipment Design
dc.subject.mesh	Cell Proliferation
dc.subject.mesh	Cell Survival
dc.subject.mesh	Gene Expression Regulation, Neoplastic
dc.subject.mesh	Human Umbilical Vein Endothelial Cells
dc.subject.mesh	A549 Cells
dc.title	Engineering a 3D microfluidic culture platform for tumor-treating field application.
dc.type	Journal Article
utslib.citation.volume	6
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	open_access	*
dc.date.updated	2022-07-13T05:49:56Z
pubs.issue	1
pubs.publication-status	Published online
pubs.volume	6
utslib.citation.issue	1

Abstract:

The limitations of current cancer therapies highlight the urgent need for a more effective therapeutic strategy. One promising approach uses an alternating electric field; however, the mechanisms involved in the disruption of the cancer cell cycle as well as the potential adverse effects on non-cancerous cells must be clarified. In this study, we present a novel microfluidic device with embedded electrodes that enables the application of an alternating electric field therapy to cancer cells in a 3D extracellular matrix. To demonstrate the potential of our system to aid in designing and testing new therapeutic approaches, cancer cells and cancer cell aggregates were cultured individually or co-cultured with endothelial cells. The metastatic potential of the cancer cells was reduced after electric field treatment. Moreover, the proliferation rate of the treated cancer cells was lower compared with that of the untreated cells, whereas the morphologies and proliferative capacities of the endothelial cells were not significantly affected. These results demonstrate that our novel system can be used to rapidly screen the effect of an alternating electric field on cancer and normal cells within an in vivo-like microenvironment with the potential to optimize treatment protocols and evaluate synergies between tumor-treating field treatment and chemotherapy.

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http://hdl.handle.net/10453/158853