Numerical and experimental study of capillary-driven flow of PCR solution in hybrid hydrophobic microfluidic networks.

Ramalingam, N; Warkiani, ME; Ramalingam, N; Keshavarzi, G; Hao-Bing, L; Hai-Qing, TG

Numerical and experimental study of capillary-driven flow of PCR solution in hybrid hydrophobic microfluidic networks.

Ramalingam, N Warkiani, ME Ramalingam, N Keshavarzi, G Hao-Bing, L Hai-Qing, TG

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Publisher:: SPRINGER
Publication Type:: Journal Article
Citation:: Biomed Microdevices, 2016, 18, (4), pp. 68
Issue Date:: 2016-08

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Field	Value	Language
dc.contributor.author	Ramalingam, N
dc.contributor.author	Warkiani, ME
dc.contributor.author	Ramalingam, N
dc.contributor.author	Keshavarzi, G
dc.contributor.author	Hao-Bing, L
dc.contributor.author	Hai-Qing, TG
dc.date.accessioned	2022-07-13T06:28:58Z
dc.date.available	2022-07-13T06:28:58Z
dc.date.issued	2016-08
dc.identifier.citation	Biomed Microdevices, 2016, 18, (4), pp. 68
dc.identifier.issn	1387-2176
dc.identifier.issn	1572-8781
dc.identifier.uri	http://hdl.handle.net/10453/158861
dc.description.abstract	Capillary-driven microfluidics is essential for development of point-of-care diagnostic micro-devices. Polymerase chain reaction (PCR)-based micro-devices are widely developed and used in such point-of-care settings. It is imperative to characterize the fluid parameters of PCR solution for designing efficient capillary-driven microfluidic networks. Generally, for numeric modelling, the fluid parameters of PCR solution are approximated to that of water. This procedure leads to inaccurate results, which are discrepant to experimental data. This paper describes mathematical modeling and experimental validation of capillary-driven flow inside Poly-(dimethyl) siloxane (PDMS)-glass hybrid micro-channels. Using experimentally measured PCR fluid parameters, the capillary meniscus displacement in PDMS-glass microfluidic ladder network is simulated using computational fluid dynamic (CFD), and experimentally verified to match with the simulated data.
dc.format	Print
dc.language	eng
dc.publisher	SPRINGER
dc.relation.ispartof	Biomed Microdevices
dc.relation.isbasedon	10.1007/s10544-016-0099-2
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0903 Biomedical Engineering, 0912 Materials Engineering
dc.subject.classification	Analytical Chemistry
dc.subject.mesh	Dimethylpolysiloxanes
dc.subject.mesh	Glass
dc.subject.mesh	Hydrophobic and Hydrophilic Interactions
dc.subject.mesh	Microfluidic Analytical Techniques
dc.subject.mesh	Microfluidics
dc.subject.mesh	Models, Theoretical
dc.subject.mesh	Nylons
dc.subject.mesh	Octoxynol
dc.subject.mesh	Point-of-Care Systems
dc.subject.mesh	Polymerase Chain Reaction
dc.subject.mesh	Solutions
dc.subject.mesh	Surface Properties
dc.subject.mesh	Octoxynol
dc.subject.mesh	Dimethylpolysiloxanes
dc.subject.mesh	Glass
dc.subject.mesh	Nylons
dc.subject.mesh	Solutions
dc.subject.mesh	Microfluidic Analytical Techniques
dc.subject.mesh	Polymerase Chain Reaction
dc.subject.mesh	Microfluidics
dc.subject.mesh	Surface Properties
dc.subject.mesh	Models, Theoretical
dc.subject.mesh	Point-of-Care Systems
dc.subject.mesh	Hydrophobic and Hydrophilic Interactions
dc.title	Numerical and experimental study of capillary-driven flow of PCR solution in hybrid hydrophobic microfluidic networks.
dc.type	Journal Article
utslib.citation.volume	18
utslib.location.activity	United States
utslib.for	0903 Biomedical Engineering
utslib.for	0912 Materials Engineering
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	closed_access	*
dc.date.updated	2022-07-13T06:28:57Z
pubs.issue	4
pubs.publication-status	Published
pubs.volume	18
utslib.citation.issue	4

Abstract:

Capillary-driven microfluidics is essential for development of point-of-care diagnostic micro-devices. Polymerase chain reaction (PCR)-based micro-devices are widely developed and used in such point-of-care settings. It is imperative to characterize the fluid parameters of PCR solution for designing efficient capillary-driven microfluidic networks. Generally, for numeric modelling, the fluid parameters of PCR solution are approximated to that of water. This procedure leads to inaccurate results, which are discrepant to experimental data. This paper describes mathematical modeling and experimental validation of capillary-driven flow inside Poly-(dimethyl) siloxane (PDMS)-glass hybrid micro-channels. Using experimentally measured PCR fluid parameters, the capillary meniscus displacement in PDMS-glass microfluidic ladder network is simulated using computational fluid dynamic (CFD), and experimentally verified to match with the simulated data.

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http://hdl.handle.net/10453/158861