A hybrid microfluidic system for regulation of neural differentiation in induced pluripotent stem cells.

Hesari, Z; Soleimani, M; Atyabi, F; Sharifdini, M; Nadri, S; Warkiani, ME; Zare, M; Dinarvand, R

A hybrid microfluidic system for regulation of neural differentiation in induced pluripotent stem cells.

Hesari, Z Soleimani, M Atyabi, F Sharifdini, M Nadri, S Warkiani, ME Zare, M Dinarvand, R

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Publisher:: WILEY
Publication Type:: Journal Article
Citation:: J Biomed Mater Res A, 2016, 104, (6), pp. 1534-1543
Issue Date:: 2016-06

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Field	Value	Language
dc.contributor.author	Hesari, Z
dc.contributor.author	Soleimani, M
dc.contributor.author	Atyabi, F
dc.contributor.author	Sharifdini, M
dc.contributor.author	Nadri, S
dc.contributor.author	Warkiani, ME
dc.contributor.author	Zare, M
dc.contributor.author	Dinarvand, R
dc.date.accessioned	2022-07-13T22:44:24Z
dc.date.available	2016-02-16
dc.date.available	2022-07-13T22:44:24Z
dc.date.issued	2016-06
dc.identifier.citation	J Biomed Mater Res A, 2016, 104, (6), pp. 1534-1543
dc.identifier.issn	1549-3296
dc.identifier.issn	1552-4965
dc.identifier.uri	http://hdl.handle.net/10453/158865
dc.description.abstract	Controlling cellular orientation, proliferation, and differentiation is valuable in designing organ replacements and directing tissue regeneration. In the present study, we developed a hybrid microfluidic system to produce a dynamic microenvironment by placing aligned PDMS microgrooves on surface of biodegradable polymers as physical guidance cues for controlling the neural differentiation of human induced pluripotent stem cells (hiPSCs). The neuronal differentiation capacity of cultured hiPSCs in the microfluidic system and other control groups was investigated using quantitative real time PCR (qPCR) and immunocytochemistry. The functionally of differentiated hiPSCs inside hybrid system's scaffolds was also evaluated on the rat hemisected spinal cord in acute phase. Implanted cell's fate was examined using tissue freeze section and the functional recovery was evaluated according to the Basso, Beattie, and Bresnahan (BBB) locomotor rating scale. Our results confirmed the differentiation of hiPSCs to neuronal cells on the microfluidic device where the expression of neuronal-specific genes was significantly higher compared to those cultured on the other systems such as plain tissue culture dishes and scaffolds without fluidic channels. Although survival and integration of implanted hiPSCs did not lead to a significant functional recovery, we believe that combination of fluidic channels with nanofiber scaffolds provides a great microenvironment for neural tissue engineering, and can be used as a powerful tool for in situ monitoring of differentiation potential of various kinds of stem cells. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1534-1543, 2016.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	WILEY
dc.relation.ispartof	J Biomed Mater Res A
dc.relation.isbasedon	10.1002/jbm.a.35689
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	03 Chemical Sciences, 06 Biological Sciences, 09 Engineering
dc.subject.mesh	Animals
dc.subject.mesh	Behavior, Animal
dc.subject.mesh	Cell Differentiation
dc.subject.mesh	Cell Survival
dc.subject.mesh	Female
dc.subject.mesh	Gene Expression Regulation
dc.subject.mesh	Green Fluorescent Proteins
dc.subject.mesh	HEK293 Cells
dc.subject.mesh	Humans
dc.subject.mesh	Immunohistochemistry
dc.subject.mesh	Induced Pluripotent Stem Cells
dc.subject.mesh	Lactic Acid
dc.subject.mesh	Microfluidics
dc.subject.mesh	Nanofibers
dc.subject.mesh	Neurons
dc.subject.mesh	Polyglycolic Acid
dc.subject.mesh	Polylactic Acid-Polyglycolic Acid Copolymer
dc.subject.mesh	Rats, Wistar
dc.subject.mesh	Real-Time Polymerase Chain Reaction
dc.subject.mesh	Spinal Cord Injuries
dc.subject.mesh	Staining and Labeling
dc.subject.mesh	Tissue Scaffolds
dc.subject.mesh	Neurons
dc.subject.mesh	Animals
dc.subject.mesh	Humans
dc.subject.mesh	Rats, Wistar
dc.subject.mesh	Spinal Cord Injuries
dc.subject.mesh	Lactic Acid
dc.subject.mesh	Polyglycolic Acid
dc.subject.mesh	Green Fluorescent Proteins
dc.subject.mesh	Immunohistochemistry
dc.subject.mesh	Staining and Labeling
dc.subject.mesh	Microfluidics
dc.subject.mesh	Behavior, Animal
dc.subject.mesh	Cell Differentiation
dc.subject.mesh	Cell Survival
dc.subject.mesh	Gene Expression Regulation
dc.subject.mesh	Female
dc.subject.mesh	Tissue Scaffolds
dc.subject.mesh	Induced Pluripotent Stem Cells
dc.subject.mesh	Nanofibers
dc.subject.mesh	HEK293 Cells
dc.subject.mesh	Real-Time Polymerase Chain Reaction
dc.subject.mesh	Polylactic Acid-Polyglycolic Acid Copolymer
dc.title	A hybrid microfluidic system for regulation of neural differentiation in induced pluripotent stem cells.
dc.type	Journal Article
utslib.citation.volume	104
utslib.location.activity	United States
utslib.for	03 Chemical Sciences
utslib.for	06 Biological Sciences
utslib.for	09 Engineering
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	closed_access	*
dc.date.updated	2022-07-13T22:44:23Z
pubs.issue	6
pubs.publication-status	Published
pubs.volume	104
utslib.citation.issue	6

Abstract:

Controlling cellular orientation, proliferation, and differentiation is valuable in designing organ replacements and directing tissue regeneration. In the present study, we developed a hybrid microfluidic system to produce a dynamic microenvironment by placing aligned PDMS microgrooves on surface of biodegradable polymers as physical guidance cues for controlling the neural differentiation of human induced pluripotent stem cells (hiPSCs). The neuronal differentiation capacity of cultured hiPSCs in the microfluidic system and other control groups was investigated using quantitative real time PCR (qPCR) and immunocytochemistry. The functionally of differentiated hiPSCs inside hybrid system's scaffolds was also evaluated on the rat hemisected spinal cord in acute phase. Implanted cell's fate was examined using tissue freeze section and the functional recovery was evaluated according to the Basso, Beattie, and Bresnahan (BBB) locomotor rating scale. Our results confirmed the differentiation of hiPSCs to neuronal cells on the microfluidic device where the expression of neuronal-specific genes was significantly higher compared to those cultured on the other systems such as plain tissue culture dishes and scaffolds without fluidic channels. Although survival and integration of implanted hiPSCs did not lead to a significant functional recovery, we believe that combination of fluidic channels with nanofiber scaffolds provides a great microenvironment for neural tissue engineering, and can be used as a powerful tool for in situ monitoring of differentiation potential of various kinds of stem cells. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1534-1543, 2016.

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http://hdl.handle.net/10453/158865