The ultra-structural organization of the elastic network in the intra- and inter-lamellar matrix of the intervertebral disc.

Tavakoli, J; Elliott, DM; Costi, JJ

The ultra-structural organization of the elastic network in the intra- and inter-lamellar matrix of the intervertebral disc.

Tavakoli, J

Elliott, DM Costi, JJ

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Publisher:: ELSEVIER SCI LTD
Publication Type:: Journal Article
Citation:: Acta Biomater, 2017, 58, pp. 269-277
Issue Date:: 2017-08

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Field	Value	Language
dc.contributor.author	Tavakoli, J https://orcid.org/0000-0002-0696-6530
dc.contributor.author	Elliott, DM
dc.contributor.author	Costi, JJ
dc.date.accessioned	2022-08-11T23:25:08Z
dc.date.available	2017-05-15
dc.date.available	2022-08-11T23:25:08Z
dc.date.issued	2017-08
dc.identifier.citation	Acta Biomater, 2017, 58, pp. 269-277
dc.identifier.issn	1742-7061
dc.identifier.issn	1878-7568
dc.identifier.uri	http://hdl.handle.net/10453/159988
dc.description.abstract	The inter-lamellar matrix (ILM)-located between adjacent lamellae of the annulus fibrosus-consists of a complex structure of elastic fibers, while elastic fibers of the intra-lamellar region are aligned predominantly parallel to the collagen fibers. The organization of elastic fibers under low magnification, in both inter- and intra-lamellar regions, was studied by light microscopic analysis of histologically prepared samples; however, little is known about their ultrastructure. An ultrastructural visualization of elastic fibers in the inter-lamellar matrix is crucial for describing their contribution to structural integrity, as well as mechanical properties of the annulus fibrosus. The aims of this study were twofold: first, to present an ultrastructural analysis of the elastic fiber network in the ILM and intra-lamellar region, including cross section (CS) and in-plane (IP) lamellae, of the AF using Scanning Electron Microscopy (SEM) and second, to -compare the elastic fiber orientation between the ILM and intra-lamellar region. Four samples (lumbar sheep discs) from adjacent sections (30μm thickness) of anterior annulus were partially digested by a developed NaOH-sonication method for visualization of elastic fibers by SEM. Elastic fiber orientation and distribution were quantified relative to the tangential to circumferential reference axis. Visualization of the ILM under high magnification revealed a dense network of elastic fibers that has not been previously described. Within the ILM, elastic fibers form a complex network, consisting of different size and shape fibers, which differed to those located in the intra-lamellar region. For both regions, the majority of fibers were oriented near 0° with respect to tangential to circumferential (TCD) direction and two minor symmetrical orientations of approximately±45°. Statistically, the orientation of elastic fibers between the ILM and intra-lamellar region was not different (p=0.171). The present study used extracellular matrix partial digestion to address significant gaps in understanding of disc microstructure and will contribute to multidisciplinary ultrastructure-function studies. STATEMENT OF SIGNIFICANCE: Visualization of the intra-lamellar matrix under high magnification revealed a dense network of elastic fibers that has not been previously described. The present study used extracellular matrix partial digestion to address significant gaps in understanding of disc microstructure and will contribute to multidisciplinary ultrastructure-function studies.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	ELSEVIER SCI LTD
dc.relation.ispartof	Acta Biomater
dc.relation.isbasedon	10.1016/j.actbio.2017.05.036
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject.classification	Biomedical Engineering
dc.subject.mesh	Animals
dc.subject.mesh	Elastic Tissue
dc.subject.mesh	Extracellular Matrix
dc.subject.mesh	Intervertebral Disc
dc.subject.mesh	Microscopy, Atomic Force
dc.subject.mesh	Sheep
dc.subject.mesh	Elastic Tissue
dc.subject.mesh	Extracellular Matrix
dc.subject.mesh	Animals
dc.subject.mesh	Sheep
dc.subject.mesh	Microscopy, Atomic Force
dc.subject.mesh	Intervertebral Disc
dc.title	The ultra-structural organization of the elastic network in the intra- and inter-lamellar matrix of the intervertebral disc.
dc.type	Journal Article
utslib.citation.volume	58
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	closed_access	*
dc.date.updated	2022-08-11T23:25:07Z
pubs.publication-status	Published
pubs.volume	58

Abstract:

The inter-lamellar matrix (ILM)-located between adjacent lamellae of the annulus fibrosus-consists of a complex structure of elastic fibers, while elastic fibers of the intra-lamellar region are aligned predominantly parallel to the collagen fibers. The organization of elastic fibers under low magnification, in both inter- and intra-lamellar regions, was studied by light microscopic analysis of histologically prepared samples; however, little is known about their ultrastructure. An ultrastructural visualization of elastic fibers in the inter-lamellar matrix is crucial for describing their contribution to structural integrity, as well as mechanical properties of the annulus fibrosus. The aims of this study were twofold: first, to present an ultrastructural analysis of the elastic fiber network in the ILM and intra-lamellar region, including cross section (CS) and in-plane (IP) lamellae, of the AF using Scanning Electron Microscopy (SEM) and second, to -compare the elastic fiber orientation between the ILM and intra-lamellar region. Four samples (lumbar sheep discs) from adjacent sections (30μm thickness) of anterior annulus were partially digested by a developed NaOH-sonication method for visualization of elastic fibers by SEM. Elastic fiber orientation and distribution were quantified relative to the tangential to circumferential reference axis. Visualization of the ILM under high magnification revealed a dense network of elastic fibers that has not been previously described. Within the ILM, elastic fibers form a complex network, consisting of different size and shape fibers, which differed to those located in the intra-lamellar region. For both regions, the majority of fibers were oriented near 0° with respect to tangential to circumferential (TCD) direction and two minor symmetrical orientations of approximately±45°. Statistically, the orientation of elastic fibers between the ILM and intra-lamellar region was not different (p=0.171). The present study used extracellular matrix partial digestion to address significant gaps in understanding of disc microstructure and will contribute to multidisciplinary ultrastructure-function studies. STATEMENT OF SIGNIFICANCE: Visualization of the intra-lamellar matrix under high magnification revealed a dense network of elastic fibers that has not been previously described. The present study used extracellular matrix partial digestion to address significant gaps in understanding of disc microstructure and will contribute to multidisciplinary ultrastructure-function studies.

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http://hdl.handle.net/10453/159988