X-ray induced singlet oxygen generation by nanoparticle-photosensitizer conjugates for photodynamic therapy: determination of singlet oxygen quantum yield.

Clement, S; Deng, W; Camilleri, E; Wilson, BC; Goldys, EM

X-ray induced singlet oxygen generation by nanoparticle-photosensitizer conjugates for photodynamic therapy: determination of singlet oxygen quantum yield.

Clement, S Deng, W

Camilleri, E Wilson, BC Goldys, EM

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Publisher:: NATURE PUBLISHING GROUP
Publication Type:: Journal Article
Citation:: Sci Rep, 2016, 6, (1), pp. 19954
Issue Date:: 2016-01-28

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Field	Value	Language
dc.contributor.author	Clement, S
dc.contributor.author	Deng, W https://orcid.org/0000-0002-9413-0978
dc.contributor.author	Camilleri, E
dc.contributor.author	Wilson, BC
dc.contributor.author	Goldys, EM
dc.date.accessioned	2022-08-15T21:57:08Z
dc.date.available	2015-12-21
dc.date.available	2022-08-15T21:57:08Z
dc.date.issued	2016-01-28
dc.identifier.citation	Sci Rep, 2016, 6, (1), pp. 19954
dc.identifier.issn	2045-2322
dc.identifier.issn	2045-2322
dc.identifier.uri	http://hdl.handle.net/10453/160265
dc.description.abstract	Singlet oxygen is a primary cytotoxic agent in photodynamic therapy. We show that CeF3 nanoparticles, pure as well as conjugated through electrostatic interaction with the photosensitizer verteporfin, are able to generate singlet oxygen as a result of UV light and 8 keV X-ray irradiation. The X-ray stimulated singlet oxygen quantum yield was determined to be 0.79 ± 0.05 for the conjugate with 31 verteporfin molecules per CeF3 nanoparticle, the highest conjugation level used. From this result we estimate the singlet oxygen dose generated from CeF3-verteporfin conjugates for a therapeutic dose of 60 Gy of ionizing radiation at energies of 6 MeV and 30 keV to be (1.2 ± 0.7) × 10(8) and (2.0 ± 0.1) × 10(9) singlet oxygen molecules per cell, respectively. These are comparable with cytotoxic doses of 5 × 10(7)-2 × 10(9) singlet oxygen molecules per cell reported in the literature for photodynamic therapy using light activation. We confirmed that the CeF3-VP conjugates enhanced cell killing with 6 MeV radiation. This work confirms the feasibility of using X- or γ- ray activated nanoparticle-photosensitizer conjugates, either to supplement the radiation treatment of cancer, or as an independent treatment modality.
dc.format	Electronic
dc.language	eng
dc.publisher	NATURE PUBLISHING GROUP
dc.relation	http://purl.org/au-research/grants/arc/CE140100003
dc.relation.ispartof	Sci Rep
dc.relation.isbasedon	10.1038/srep19954
dc.rights	info:eu-repo/semantics/openAccess
dc.subject.mesh	Cell Line
dc.subject.mesh	Cell Survival
dc.subject.mesh	Humans
dc.subject.mesh	Nanoparticles
dc.subject.mesh	Photochemotherapy
dc.subject.mesh	Photosensitizing Agents
dc.subject.mesh	Singlet Oxygen
dc.subject.mesh	Spectroscopy, Fourier Transform Infrared
dc.subject.mesh	X-Rays
dc.subject.mesh	Cell Line
dc.subject.mesh	Humans
dc.subject.mesh	Singlet Oxygen
dc.subject.mesh	Photosensitizing Agents
dc.subject.mesh	Photochemotherapy
dc.subject.mesh	Spectroscopy, Fourier Transform Infrared
dc.subject.mesh	Cell Survival
dc.subject.mesh	X-Rays
dc.subject.mesh	Nanoparticles
dc.title	X-ray induced singlet oxygen generation by nanoparticle-photosensitizer conjugates for photodynamic therapy: determination of singlet oxygen quantum yield.
dc.type	Journal Article
utslib.citation.volume	6
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
utslib.copyright.status	open_access	*
dc.date.updated	2022-08-15T21:57:06Z
pubs.issue	1
pubs.publication-status	Published online
pubs.volume	6
utslib.citation.issue	1

Abstract:

Singlet oxygen is a primary cytotoxic agent in photodynamic therapy. We show that CeF3 nanoparticles, pure as well as conjugated through electrostatic interaction with the photosensitizer verteporfin, are able to generate singlet oxygen as a result of UV light and 8 keV X-ray irradiation. The X-ray stimulated singlet oxygen quantum yield was determined to be 0.79 ± 0.05 for the conjugate with 31 verteporfin molecules per CeF3 nanoparticle, the highest conjugation level used. From this result we estimate the singlet oxygen dose generated from CeF3-verteporfin conjugates for a therapeutic dose of 60 Gy of ionizing radiation at energies of 6 MeV and 30 keV to be (1.2 ± 0.7) × 10(8) and (2.0 ± 0.1) × 10(9) singlet oxygen molecules per cell, respectively. These are comparable with cytotoxic doses of 5 × 10(7)-2 × 10(9) singlet oxygen molecules per cell reported in the literature for photodynamic therapy using light activation. We confirmed that the CeF3-VP conjugates enhanced cell killing with 6 MeV radiation. This work confirms the feasibility of using X- or γ- ray activated nanoparticle-photosensitizer conjugates, either to supplement the radiation treatment of cancer, or as an independent treatment modality.

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http://hdl.handle.net/10453/160265