Fructose-Coated Nanodiamonds: Promising Platforms for Treatment of Human Breast Cancer

Zhao, JC; Lai, HW; Lu, HX; Barner-Kowollik, C; Stenzel, MH; Xiao, P

Fructose-Coated Nanodiamonds: Promising Platforms for Treatment of Human Breast Cancer

Zhao, JC Lai, HW Lu, HX Barner-Kowollik, C Stenzel, MH Xiao, P

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Publisher:: AMER CHEMICAL SOC
Publication Type:: Journal Article
Citation:: Biomacromolecules, 2016, 17, (9), pp. 2946-2955
Issue Date:: 2016

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Field	Value	Language
dc.contributor.author	Zhao, JC
dc.contributor.author	Lai, HW
dc.contributor.author	Lu, HX
dc.contributor.author	Barner-Kowollik, C
dc.contributor.author	Stenzel, MH
dc.contributor.author	Xiao, P
dc.date.accessioned	2022-08-20T22:09:13Z
dc.date.available	2022-08-20T22:09:13Z
dc.date.issued	2016
dc.identifier.citation	Biomacromolecules, 2016, 17, (9), pp. 2946-2955
dc.identifier.issn	1525-7797
dc.identifier.issn	1526-4602
dc.identifier.uri	http://hdl.handle.net/10453/160586
dc.description.abstract	Well-defined carboxyl end-functionalized glycopolymer Poly(1-O-methacryloyl-2,3:4,5-di-O-isopropylidene-β-d-fructopyranose) (Poly(1-O-MAipFru)62) has been prepared via reversible addition-fragmentation chain transfer polymerization and grafted onto the surface of amine-functionalized nanodiamonds via a simple conjugation reaction. The properties of the nanodiamond-polymer hybrid materials ND-Poly(1-O-MAFru)62 are investigated using infrared spectroscopy, thermogravimetric analysis, dynamic light scattering, and transmission electron microscopy. The dispersibility of the nanodiamonds in aqueous solutions is significantly improved after the grafting of the glycopolymer. More interestingly, the cytotoxicity of amine-functionalized nanodiamonds is significantly decreased after decoration with the glycopolymer even at a high concentration (125 μg/mL). The nanodiamonds were loaded with doxorubicin to create a bioactive drug delivery carrier. The release of doxorubicin was faster in media of pH 5 than media of pH 7.4. The nanodiamond drug delivery systems with doxorubicin are used to treat breast cancer cells in 2D and 3D models. Although the 2D cell culture results indicate that all nanodiamonds-doxorubicin complexes are significantly less toxic than free doxorubicin, the glycopolymer-coated nanodiamonds-doxorubicin show higher cytotoxicity than free doxorubicin in the 3D spheroids after treatment for 8 days. The enhanced cytotoxicity of Poly(1-O-MAFru)62-ND-Dox in 3D spheroids may result from the sustained drug release and deep penetration of these nanocarriers, which play a role as a "Trojan Horse". The massive cell death after 8-day incubation with Poly(1-O-MAFru)62-ND-Dox demonstrates that glycopolymer-coated nanodiamonds can be promising platforms for breast cancer therapy.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	AMER CHEMICAL SOC
dc.relation.ispartof	Biomacromolecules
dc.relation.isbasedon	10.1021/acs.biomac.6b00754
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	03 Chemical Sciences, 06 Biological Sciences, 09 Engineering
dc.subject.classification	Polymers
dc.subject.mesh	Antibiotics, Antineoplastic
dc.subject.mesh	Breast Neoplasms
dc.subject.mesh	Cell Survival
dc.subject.mesh	Doxorubicin
dc.subject.mesh	Drug Delivery Systems
dc.subject.mesh	Drug Liberation
dc.subject.mesh	Female
dc.subject.mesh	Fructose
dc.subject.mesh	Humans
dc.subject.mesh	Nanodiamonds
dc.subject.mesh	Polymers
dc.subject.mesh	Spheroids, Cellular
dc.subject.mesh	Tumor Cells, Cultured
dc.subject.mesh	Spheroids, Cellular
dc.subject.mesh	Tumor Cells, Cultured
dc.subject.mesh	Humans
dc.subject.mesh	Breast Neoplasms
dc.subject.mesh	Doxorubicin
dc.subject.mesh	Polymers
dc.subject.mesh	Fructose
dc.subject.mesh	Antibiotics, Antineoplastic
dc.subject.mesh	Drug Delivery Systems
dc.subject.mesh	Cell Survival
dc.subject.mesh	Female
dc.subject.mesh	Nanodiamonds
dc.subject.mesh	Drug Liberation
dc.title	Fructose-Coated Nanodiamonds: Promising Platforms for Treatment of Human Breast Cancer
dc.type	Journal Article
utslib.citation.volume	17
utslib.location.activity	United States
utslib.for	03 Chemical Sciences
utslib.for	06 Biological Sciences
utslib.for	09 Engineering
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	closed_access	*
dc.date.updated	2022-08-20T22:09:08Z
pubs.issue	9
pubs.publication-status	Published
pubs.volume	17
utslib.citation.issue	9

Abstract:

Well-defined carboxyl end-functionalized glycopolymer Poly(1-O-methacryloyl-2,3:4,5-di-O-isopropylidene-β-d-fructopyranose) (Poly(1-O-MAipFru)62) has been prepared via reversible addition-fragmentation chain transfer polymerization and grafted onto the surface of amine-functionalized nanodiamonds via a simple conjugation reaction. The properties of the nanodiamond-polymer hybrid materials ND-Poly(1-O-MAFru)62 are investigated using infrared spectroscopy, thermogravimetric analysis, dynamic light scattering, and transmission electron microscopy. The dispersibility of the nanodiamonds in aqueous solutions is significantly improved after the grafting of the glycopolymer. More interestingly, the cytotoxicity of amine-functionalized nanodiamonds is significantly decreased after decoration with the glycopolymer even at a high concentration (125 μg/mL). The nanodiamonds were loaded with doxorubicin to create a bioactive drug delivery carrier. The release of doxorubicin was faster in media of pH 5 than media of pH 7.4. The nanodiamond drug delivery systems with doxorubicin are used to treat breast cancer cells in 2D and 3D models. Although the 2D cell culture results indicate that all nanodiamonds-doxorubicin complexes are significantly less toxic than free doxorubicin, the glycopolymer-coated nanodiamonds-doxorubicin show higher cytotoxicity than free doxorubicin in the 3D spheroids after treatment for 8 days. The enhanced cytotoxicity of Poly(1-O-MAFru)62-ND-Dox in 3D spheroids may result from the sustained drug release and deep penetration of these nanocarriers, which play a role as a "Trojan Horse". The massive cell death after 8-day incubation with Poly(1-O-MAFru)62-ND-Dox demonstrates that glycopolymer-coated nanodiamonds can be promising platforms for breast cancer therapy.

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http://hdl.handle.net/10453/160586