Direct Correlation Between Zeta Potential and Cellular Uptake of Poly(methacrylic acid) Post-Modified with Guanidinium Functionalities

Khine, YY; Callari, M; Lu, H; Stenzel, MH

Direct Correlation Between Zeta Potential and Cellular Uptake of Poly(methacrylic acid) Post-Modified with Guanidinium Functionalities

Khine, YY Callari, M Lu, H

Stenzel, MH

Permalink

Publisher:: WILEY-V C H VERLAG GMBH
Publication Type:: Journal Article
Citation:: Macromolecular Chemistry and Physics, 2016, 217, (20), pp. 2302-2309
Issue Date:: 2016

Closed Access

	Filename	Description	Size
	Macro Chemistry Physics - 2016 - Khine - Direct Correlation Between Zeta Potential and Cellular Uptake of Poly.pdf	Published version	973.07 kB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Khine, YY
dc.contributor.author	Callari, M
dc.contributor.author	Lu, H https://orcid.org/0000-0001-6932-1900
dc.contributor.author	Stenzel, MH
dc.date.accessioned	2022-08-21T22:03:33Z
dc.date.available	2022-08-21T22:03:33Z
dc.date.issued	2016
dc.identifier.citation	Macromolecular Chemistry and Physics, 2016, 217, (20), pp. 2302-2309
dc.identifier.issn	1022-1352
dc.identifier.issn	1521-3935
dc.identifier.uri	http://hdl.handle.net/10453/160625
dc.description.abstract	Cellular uptake is identified as one of the key parameters to the successful delivery of drugs into cancer cells. In this study, the effect of the ratio of negative and positive charges on a polymer on the amount of uptake is studied. Poly(methacrylic acid), prepared by RAFT polymerization, is post-functionalized with agmatine in water to various degrees. When agmatine ratio is increased to 1, the conjugation efficiency is observed to be ≈63% indicating potentially some steric hindrance or excessive repulsion by the positive charged guanidinium functionality. The resulting zeta potential, ranging from −40 to −14 mV, is in linear correlation with the degree of functionalization. The resulting polymers are found to be non-toxic to A2780 ovarian cancer cells. The cellular uptake is subsequently studied by laser scanning confocal fluorescent microscopy, flow cytometry, and by analysis of the fluorescence intensity of the remaining solution after incubation with A2780 ovarian cancer cells. An almost linear relationship between zeta potential and cellular uptake is observed. (Figure presented.).
dc.language	English
dc.publisher	WILEY-V C H VERLAG GMBH
dc.relation.ispartof	Macromolecular Chemistry and Physics
dc.relation.isbasedon	10.1002/macp.201600161
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0303 Macromolecular and Materials Chemistry, 0912 Materials Engineering
dc.subject.classification	Polymers
dc.title	Direct Correlation Between Zeta Potential and Cellular Uptake of Poly(methacrylic acid) Post-Modified with Guanidinium Functionalities
dc.type	Journal Article
utslib.citation.volume	217
utslib.for	0303 Macromolecular and Materials Chemistry
utslib.for	0912 Materials Engineering
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	closed_access	*
dc.date.updated	2022-08-21T22:03:32Z
pubs.issue	20
pubs.publication-status	Published
pubs.volume	217
utslib.citation.issue	20

Abstract:

Cellular uptake is identified as one of the key parameters to the successful delivery of drugs into cancer cells. In this study, the effect of the ratio of negative and positive charges on a polymer on the amount of uptake is studied. Poly(methacrylic acid), prepared by RAFT polymerization, is post-functionalized with agmatine in water to various degrees. When agmatine ratio is increased to 1, the conjugation efficiency is observed to be ≈63% indicating potentially some steric hindrance or excessive repulsion by the positive charged guanidinium functionality. The resulting zeta potential, ranging from −40 to −14 mV, is in linear correlation with the degree of functionalization. The resulting polymers are found to be non-toxic to A2780 ovarian cancer cells. The cellular uptake is subsequently studied by laser scanning confocal fluorescent microscopy, flow cytometry, and by analysis of the fluorescence intensity of the remaining solution after incubation with A2780 ovarian cancer cells. An almost linear relationship between zeta potential and cellular uptake is observed. (Figure presented.).

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/160625