Cytotoxic T lymphocytes and CD4 epitope mutations in the pre-core/core region of hepatitis B virus in chronic hepatitis B carriers in Northeast Iran.

Zhand, S; Tabarraei, A; Nazari, A; Moradi, A

Cytotoxic T lymphocytes and CD4 epitope mutations in the pre-core/core region of hepatitis B virus in chronic hepatitis B carriers in Northeast Iran.

Zhand, S Tabarraei, A Nazari, A Moradi, A

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Publisher:: Springer
Publication Type:: Journal Article
Citation:: Indian Journal of Gastroenterology, 2017, 36, (4), pp. 253-257
Issue Date:: 2017-07

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Field	Value	Language
dc.contributor.author	Zhand, S
dc.contributor.author	Tabarraei, A
dc.contributor.author	Nazari, A
dc.contributor.author	Moradi, A
dc.date.accessioned	2022-08-26T05:22:00Z
dc.date.available	2017-06-30
dc.date.available	2022-08-26T05:22:00Z
dc.date.issued	2017-07
dc.identifier.citation	Indian Journal of Gastroenterology, 2017, 36, (4), pp. 253-257
dc.identifier.issn	0254-8860
dc.identifier.issn	0975-0711
dc.identifier.uri	http://hdl.handle.net/10453/160905
dc.description.abstract	BACKGROUNDS: Hepatitis B virus (HBV) is vulnerable to many various mutations. Those within epitopes recognized by sensitized T cells may influence the re-emergence of the virus. This study was designed to investigate the mutation in immune epitope regions of HBV pre-core/core among chronic HBV patients of Golestan province, Northeast Iran. METHODS: In 120 chronic HBV carriers, HBV DNA was extracted from blood plasma samples and PCR was done using specific primers. Direct sequencing and alignment of the pre-core/core region were applied using reference sequence from Gene Bank database (Accession Number AB033559). RESULTS: The study showed 27 inferred amino acid substitutions, 9 of which (33.3%) were in CD4 and 2 (7.4%) in cytotoxic T lymphocytes' (CTL) epitopes and 16 other mutations (59.2%) were observed in other regions. CONCLUSIONS: CTL escape mutations were not commonly observed in pre-core/core sequences of chronic HBV carriers in the locale of study. It can be concluded that most of the inferred amino acid substitutions occur in different immune epitopes other than CTL and CD4.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Springer
dc.relation.ispartof	Indian Journal of Gastroenterology
dc.relation.isbasedon	10.1007/s12664-017-0767-z
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	1103 Clinical Sciences
dc.subject.mesh	Amino Acid Substitution
dc.subject.mesh	Carrier State
dc.subject.mesh	CD4 Antigens
dc.subject.mesh	Epitopes
dc.subject.mesh	Hepatitis B virus
dc.subject.mesh	Hepatitis B, Chronic
dc.subject.mesh	Humans
dc.subject.mesh	Iran
dc.subject.mesh	Point Mutation
dc.subject.mesh	T-Lymphocytes, Cytotoxic
dc.subject.mesh	Amino Acid Substitution
dc.subject.mesh	CD4 Antigens
dc.subject.mesh	Carrier State
dc.subject.mesh	Epitopes
dc.subject.mesh	Hepatitis B virus
dc.subject.mesh	Hepatitis B, Chronic
dc.subject.mesh	Humans
dc.subject.mesh	Iran
dc.subject.mesh	Point Mutation
dc.subject.mesh	T-Lymphocytes, Cytotoxic
dc.subject.mesh	T-Lymphocytes, Cytotoxic
dc.subject.mesh	Humans
dc.subject.mesh	Hepatitis B virus
dc.subject.mesh	Hepatitis B, Chronic
dc.subject.mesh	Epitopes
dc.subject.mesh	Amino Acid Substitution
dc.subject.mesh	Carrier State
dc.subject.mesh	Point Mutation
dc.subject.mesh	Iran
dc.subject.mesh	CD4 Antigens
dc.title	Cytotoxic T lymphocytes and CD4 epitope mutations in the pre-core/core region of hepatitis B virus in chronic hepatitis B carriers in Northeast Iran.
dc.type	Journal Article
utslib.citation.volume	36
utslib.location.activity	India
utslib.for	1103 Clinical Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2022-08-26T05:21:58Z
pubs.issue	4
pubs.publication-status	Published
pubs.volume	36
utslib.citation.issue	4

Abstract:

BACKGROUNDS: Hepatitis B virus (HBV) is vulnerable to many various mutations. Those within epitopes recognized by sensitized T cells may influence the re-emergence of the virus. This study was designed to investigate the mutation in immune epitope regions of HBV pre-core/core among chronic HBV patients of Golestan province, Northeast Iran. METHODS: In 120 chronic HBV carriers, HBV DNA was extracted from blood plasma samples and PCR was done using specific primers. Direct sequencing and alignment of the pre-core/core region were applied using reference sequence from Gene Bank database (Accession Number AB033559). RESULTS: The study showed 27 inferred amino acid substitutions, 9 of which (33.3%) were in CD4 and 2 (7.4%) in cytotoxic T lymphocytes' (CTL) epitopes and 16 other mutations (59.2%) were observed in other regions. CONCLUSIONS: CTL escape mutations were not commonly observed in pre-core/core sequences of chronic HBV carriers in the locale of study. It can be concluded that most of the inferred amino acid substitutions occur in different immune epitopes other than CTL and CD4.

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http://hdl.handle.net/10453/160905