Mutations in Hepatitis-B X-Gene Region: Chronic Hepatitis-B versus Cirrhosis.

Salarnia, F; Besharat, S; Zhand, S; Javid, N; Khodabakhshi, B; Moradi, A

Mutations in Hepatitis-B X-Gene Region: Chronic Hepatitis-B versus Cirrhosis.

Salarnia, F Besharat, S Zhand, S Javid, N Khodabakhshi, B Moradi, A

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Publisher:: Dr. Hemant Jain and Premchand Shantidevi Research foundation
Publication Type:: Journal Article
Citation:: Journal of Clinical and Diagnostic Research, 2017, 11, (3), pp. OC31-OC34
Issue Date:: 2017-03

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Field	Value	Language
dc.contributor.author	Salarnia, F
dc.contributor.author	Besharat, S
dc.contributor.author	Zhand, S
dc.contributor.author	Javid, N
dc.contributor.author	Khodabakhshi, B
dc.contributor.author	Moradi, A
dc.date.accessioned	2022-08-26T05:45:13Z
dc.date.available	2016-11-28
dc.date.available	2022-08-26T05:45:13Z
dc.date.issued	2017-03
dc.identifier.citation	Journal of Clinical and Diagnostic Research, 2017, 11, (3), pp. OC31-OC34
dc.identifier.issn	2249-782X
dc.identifier.issn	0973-709X
dc.identifier.uri	http://hdl.handle.net/10453/160909
dc.description.abstract	INTRODUCTION: Specific mutations in Hepatitis-B Virus (HBV) genome would proceed the development of chronic hepatitis B to more serious consequences like cirrhosis and end-stage liver disease. AIM: This study was designed to detect deletion and insertion mutational patterns in the X-gene region in a population of chronic HBV and related cirrhosis patients. MATERIALS AND METHODS: Sixty eight chronic HBV patients and 34 HBV-related cirrhotics were recruited from the eligible cases (N=50) referred to the academic hospitals of Gorgan city, Northeast of Iran, between Jan 2011 to Dec 2013. The HBx region was amplified by semi-nested PCR using serum samples and analyzed by sequencing. RESULTS: Our findings showed deletions and insertions in the C-terminal of HBx of the cirrhotic group and 8 bp found in two chronic HBV cases (2.9%). We detected 15 types of deletions in cirrhotic cases such as 1762-1768, 1763-1770, 1769-1773 and T1771/A1775. CONCLUSION: We found that the frequencies of deletion and insertion mutations in C-terminal of X-gene were more seen in cirrhotic patients comparing to chronic HBV cases in our area of study.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Dr. Hemant Jain and Premchand Shantidevi Research foundation
dc.relation.ispartof	Journal of Clinical and Diagnostic Research
dc.relation.isbasedon	10.7860/JCDR/2017/22570.9498
dc.rights	info:eu-repo/semantics/closedAccess
dc.title	Mutations in Hepatitis-B X-Gene Region: Chronic Hepatitis-B versus Cirrhosis.
dc.type	Journal Article
utslib.citation.volume	11
utslib.location.activity	India
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2022-08-26T05:45:12Z
pubs.issue	3
pubs.publication-status	Published
pubs.volume	11
utslib.citation.issue	3

Abstract:

INTRODUCTION: Specific mutations in Hepatitis-B Virus (HBV) genome would proceed the development of chronic hepatitis B to more serious consequences like cirrhosis and end-stage liver disease. AIM: This study was designed to detect deletion and insertion mutational patterns in the X-gene region in a population of chronic HBV and related cirrhosis patients. MATERIALS AND METHODS: Sixty eight chronic HBV patients and 34 HBV-related cirrhotics were recruited from the eligible cases (N=50) referred to the academic hospitals of Gorgan city, Northeast of Iran, between Jan 2011 to Dec 2013. The HBx region was amplified by semi-nested PCR using serum samples and analyzed by sequencing. RESULTS: Our findings showed deletions and insertions in the C-terminal of HBx of the cirrhotic group and 8 bp found in two chronic HBV cases (2.9%). We detected 15 types of deletions in cirrhotic cases such as 1762-1768, 1763-1770, 1769-1773 and T1771/A1775. CONCLUSION: We found that the frequencies of deletion and insertion mutations in C-terminal of X-gene were more seen in cirrhotic patients comparing to chronic HBV cases in our area of study.

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http://hdl.handle.net/10453/160909