Molecular Epidemiology of Hepatitis C Virus Genotypes in Patients with Thalassemia Major in Golestan Province, Iran

Zhand, S; Bazuori, M; Tabarraei, A; Moradi, A

Molecular Epidemiology of Hepatitis C Virus Genotypes in Patients with Thalassemia Major in Golestan Province, Iran

Zhand, S Bazuori, M Tabarraei, A Moradi, A

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Publisher:: CASRP: Center of Advanced Scientific Research and Publications
Publication Type:: Journal Article
Citation:: Journal of Clinical and Basic Research, 2017, 1, (1), pp. 1-10
Issue Date:: 2017-05-01

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Field	Value	Language
dc.contributor.author	Zhand, S
dc.contributor.author	Bazuori, M
dc.contributor.author	Tabarraei, A
dc.contributor.author	Moradi, A
dc.date.accessioned	2022-08-29T02:37:30Z
dc.date.available	2022-08-29T02:37:30Z
dc.date.issued	2017-05-01
dc.identifier.citation	Journal of Clinical and Basic Research, 2017, 1, (1), pp. 1-10
dc.identifier.issn	2538-3736
dc.identifier.issn	2538-3736
dc.identifier.uri	http://hdl.handle.net/10453/161005
dc.description.abstract	Introduction: In Iran, hepatitis C virus (HCV) is the most prevalent cause of chronic hepatitis and cirrhosis in patients with hemophilia, thalassemia, and renal failure. Recent studies suggest that patients infected with different HCV genotypes have different clinical profiles, severity of liver disease and response to therapy. Several molecular methods targeting different HCV genomic regions have been introduced for genotyping. Direct sequencing of amplified PCR products is the gold standard method, followed by phylogenetic analysis of clinical material. The aim of this study was to determine genotypes of HCV-infected patients with thalassemia in Golestan Province, Iran. Materials and Methods: This cross-sectional study included 217 patients (mean age: 21.82 ± 16 years, 50.7% male) with thalassemia major. Enzyme-linked immunosorbent assay (ELISA) was used for detection of HCV antibodies. Positive HCV-Ab samples were confirmed by reverse transcription polymerase chain reaction (RT-PCR) and sequencing. HCV genotypes were determined by aligning nucleotide sequences of patients with the standard nucleotide sequences obtained from GenBank (accession number: AB520610). Results: Of 217 patients with thalassemia major, 14 (6.45%) were found as anti-HCV-positive in the ELISA test. Among them, two patients (14.28%) had positive RT-PCR results. In addition, all patients were infected with HCV genotype 1a. Conclusions: Genotype 1a is the predominant HCV genotype in patients with thalassemia major in the Golestan province, Iran.
dc.language	en
dc.publisher	CASRP: Center of Advanced Scientific Research and Publications
dc.relation.ispartof	Journal of Clinical and Basic Research
dc.relation.isbasedon	10.18869/acadpub.jcbr.1.1.1
dc.rights	info:eu-repo/semantics/closedAccess
dc.title	Molecular Epidemiology of Hepatitis C Virus Genotypes in Patients with Thalassemia Major in Golestan Province, Iran
dc.type	Journal Article
utslib.citation.volume	1
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2022-08-29T02:37:29Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	1
utslib.citation.issue	1

Abstract:

Introduction: In Iran, hepatitis C virus (HCV) is the most prevalent cause of chronic hepatitis and cirrhosis in patients with hemophilia, thalassemia, and renal failure. Recent studies suggest that patients infected with different HCV genotypes have different clinical profiles, severity of liver disease and response to therapy. Several molecular methods targeting different HCV genomic regions have been introduced for genotyping. Direct sequencing of amplified PCR products is the gold standard method, followed by phylogenetic analysis of clinical material. The aim of this study was to determine genotypes of HCV-infected patients with thalassemia in Golestan Province, Iran. Materials and Methods: This cross-sectional study included 217 patients (mean age: 21.82 ± 16 years, 50.7% male) with thalassemia major. Enzyme-linked immunosorbent assay (ELISA) was used for detection of HCV antibodies. Positive HCV-Ab samples were confirmed by reverse transcription polymerase chain reaction (RT-PCR) and sequencing. HCV genotypes were determined by aligning nucleotide sequences of patients with the standard nucleotide sequences obtained from GenBank (accession number: AB520610). Results: Of 217 patients with thalassemia major, 14 (6.45%) were found as anti-HCV-positive in the ELISA test. Among them, two patients (14.28%) had positive RT-PCR results. In addition, all patients were infected with HCV genotype 1a. Conclusions: Genotype 1a is the predominant HCV genotype in patients with thalassemia major in the Golestan province, Iran.

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http://hdl.handle.net/10453/161005