Development of a rapid matrix digestion technique for ultrastructural analysis of elastic fibers in the intervertebral disc.

Tavakoli, J; Costi, JJ

Development of a rapid matrix digestion technique for ultrastructural analysis of elastic fibers in the intervertebral disc.

Tavakoli, J

Costi, JJ

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Publisher:: Elsevier
Publication Type:: Journal Article
Citation:: Journal of The Mechanical Behavior of Biomedical Materials, 2017, 71, pp. 175-183
Issue Date:: 2017-07

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Field	Value	Language
dc.contributor.author	Tavakoli, J https://orcid.org/0000-0002-0696-6530
dc.contributor.author	Costi, JJ
dc.date.accessioned	2022-08-30T02:15:44Z
dc.date.available	2017-03-19
dc.date.available	2022-08-30T02:15:44Z
dc.date.issued	2017-07
dc.identifier.citation	Journal of The Mechanical Behavior of Biomedical Materials, 2017, 71, pp. 175-183
dc.identifier.issn	1751-6161
dc.identifier.issn	1878-0180
dc.identifier.uri	http://hdl.handle.net/10453/161092
dc.description.abstract	Collagen and elastic fibers are two major fibrous constituents of the annulus fibrosus (AF) in the disc that contribute to its mechanical and viscoelastic properties. It was thought that elastic fibers play no substantial role in the function and properties of the disc as these fibers were irregularly distributed. Studies that have revealed highly organized elastic fibers with different regional orientation and distribution, while being strongly crosslinked with matrix, suggesting their contribution to disc structure-function properties. These studies that were performed by light microscopic analysis of histologically prepared samples, have not been able to reveal the fine-scale architectural details of the elastic fiber network. Since elastic fibers are intermingled with other fibrous components of the disc and mostly obscured by the extracellular matrix, it is difficult to demonstrate their ultra-structural organization using scanning electron microscopy (SEM). Therefore the aim of this study was to develop a rapid matrix digestion technique for ultrastructural analysis of the disc elastic fibers. This study provides a new method for fundamental visualization of elastic fibers and their architecture in the disc. Through the ultra-structural analysis, the relationship between structure and function, as well as the role of elastic fibers on AF mechanical properties can be studied. This method may be used to develop a three-dimensional map of elastic fibers distribution within the disc, which would provide valuable information for designing tissue engineered scaffolds for AF repair and replacement.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Elsevier
dc.relation.ispartof	Journal of The Mechanical Behavior of Biomedical Materials
dc.relation.isbasedon	10.1016/j.jmbbm.2017.03.014
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0903 Biomedical Engineering, 0912 Materials Engineering, 0913 Mechanical Engineering
dc.subject.classification	Biomedical Engineering
dc.subject.mesh	Annulus Fibrosus
dc.subject.mesh	Collagen
dc.subject.mesh	Elastic Tissue
dc.subject.mesh	Extracellular Matrix
dc.subject.mesh	Humans
dc.subject.mesh	Intervertebral Disc
dc.subject.mesh	Microscopy, Electron, Scanning
dc.subject.mesh	Annulus Fibrosus
dc.subject.mesh	Collagen
dc.subject.mesh	Elastic Tissue
dc.subject.mesh	Extracellular Matrix
dc.subject.mesh	Humans
dc.subject.mesh	Intervertebral Disc
dc.subject.mesh	Microscopy, Electron, Scanning
dc.subject.mesh	Elastic Tissue
dc.subject.mesh	Extracellular Matrix
dc.subject.mesh	Humans
dc.subject.mesh	Collagen
dc.subject.mesh	Microscopy, Electron, Scanning
dc.subject.mesh	Intervertebral Disc
dc.subject.mesh	Annulus Fibrosus
dc.title	Development of a rapid matrix digestion technique for ultrastructural analysis of elastic fibers in the intervertebral disc.
dc.type	Journal Article
utslib.citation.volume	71
utslib.location.activity	Netherlands
utslib.for	0903 Biomedical Engineering
utslib.for	0912 Materials Engineering
utslib.for	0913 Mechanical Engineering
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2022-08-30T02:15:41Z
pubs.publication-status	Published
pubs.volume	71

Abstract:

Collagen and elastic fibers are two major fibrous constituents of the annulus fibrosus (AF) in the disc that contribute to its mechanical and viscoelastic properties. It was thought that elastic fibers play no substantial role in the function and properties of the disc as these fibers were irregularly distributed. Studies that have revealed highly organized elastic fibers with different regional orientation and distribution, while being strongly crosslinked with matrix, suggesting their contribution to disc structure-function properties. These studies that were performed by light microscopic analysis of histologically prepared samples, have not been able to reveal the fine-scale architectural details of the elastic fiber network. Since elastic fibers are intermingled with other fibrous components of the disc and mostly obscured by the extracellular matrix, it is difficult to demonstrate their ultra-structural organization using scanning electron microscopy (SEM). Therefore the aim of this study was to develop a rapid matrix digestion technique for ultrastructural analysis of the disc elastic fibers. This study provides a new method for fundamental visualization of elastic fibers and their architecture in the disc. Through the ultra-structural analysis, the relationship between structure and function, as well as the role of elastic fibers on AF mechanical properties can be studied. This method may be used to develop a three-dimensional map of elastic fibers distribution within the disc, which would provide valuable information for designing tissue engineered scaffolds for AF repair and replacement.

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http://hdl.handle.net/10453/161092