A reformative shear precipitation procedure for the fabrication of vancomycin-loaded poly(lactide-co-glycolide) microspheres.

Wang, L; Yang, Q; Chen, Y; Chai, Y; Li, JJ; Du, L; Tan, R; Yang, S; Tu, M; Yu, B

A reformative shear precipitation procedure for the fabrication of vancomycin-loaded poly(lactide-co-glycolide) microspheres.

Wang, L Yang, Q Chen, Y Chai, Y Li, JJ Du, L Tan, R Yang, S Tu, M Yu, B

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Publisher:: SAGE Publications
Publication Type:: Journal Article
Citation:: Journal of Biomaterials Applications, 2017, 31, (7), pp. 995-1009
Issue Date:: 2017-02

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Field	Value	Language
dc.contributor.author	Wang, L
dc.contributor.author	Yang, Q
dc.contributor.author	Chen, Y
dc.contributor.author	Chai, Y
dc.contributor.author	Li, JJ
dc.contributor.author	Du, L
dc.contributor.author	Tan, R
dc.contributor.author	Yang, S
dc.contributor.author	Tu, M
dc.contributor.author	Yu, B
dc.date.accessioned	2022-09-01T05:59:34Z
dc.date.available	2022-09-01T05:59:34Z
dc.date.issued	2017-02
dc.identifier.citation	Journal of Biomaterials Applications, 2017, 31, (7), pp. 995-1009
dc.identifier.issn	0885-3282
dc.identifier.issn	1530-8022
dc.identifier.uri	http://hdl.handle.net/10453/161193
dc.description.abstract	This study reports the encapsulation of vancomycin, as a model hydrophilic drug, into poly(lactide-co-glycolide) microspheres using a novel reformative shear precipitation procedure. In contrast to the external aqueous phase used in the conventional microencapsulation technique based on emulsion solvent evaporation/extraction, the reformative shear precipitation procedure explored in this study uses a shear medium composed of glycerol as the viscous medium and ethanol as polymer antisolvent, which is relatively immiscible with the hydrophilic drug. This limits drug diffusion and leads to rapid microsphere solidification, which allows a large proportion of the hydrophilic drug to be encapsulated within the microspheres. The influence of various processing parameters, including polymer concentration, volume ratio of ethanol to glycerol in the shear medium, volume of aqueous drug solution, initial drug loading, and injecting rate of the drug-polymer emulsion, on the encapsulation efficiency and characteristics of resulting microspheres were investigated. The morphology and release characteristics, as well as mechanical, in vitro and in vivo behaviour of vancomycin-loaded poly(lactide-co-glycolide) microspheres prepared using the novel procedure were also investigated. The results demonstrated that the reformative shear precipitation procedure could achieve the loading of hydrophilic drugs into poly(lactide-co-glycolide) microspheres with high encapsulation efficiency, and the success of the procedure was largely influenced by the volume ratio of ethanol to glycerol in the shear medium. Vancomycin-loaded poly(lactide-co-glycolide) microspheres prepared using this procedure demonstrated favourable mechanical characteristics, antibacterial activity, and in vivo degradation behaviour which suggested their suitability for use as a sustained delivery system.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	SAGE Publications
dc.relation.ispartof	Journal of Biomaterials Applications
dc.relation.isbasedon	10.1177/0885328216689199
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0903 Biomedical Engineering, 0912 Materials Engineering
dc.subject.classification	Biomedical Engineering
dc.subject.mesh	Anti-Bacterial Agents
dc.subject.mesh	Capsules
dc.subject.mesh	Cell Survival
dc.subject.mesh	Compressive Strength
dc.subject.mesh	Diffusion
dc.subject.mesh	Drug Compounding
dc.subject.mesh	Fractional Precipitation
dc.subject.mesh	Hydrophobic and Hydrophilic Interactions
dc.subject.mesh	Polyglactin 910
dc.subject.mesh	Shear Strength
dc.subject.mesh	Staphylococcus aureus
dc.subject.mesh	Vancomycin
dc.subject.mesh	Anti-Bacterial Agents
dc.subject.mesh	Capsules
dc.subject.mesh	Cell Survival
dc.subject.mesh	Compressive Strength
dc.subject.mesh	Diffusion
dc.subject.mesh	Drug Compounding
dc.subject.mesh	Fractional Precipitation
dc.subject.mesh	Hydrophobic and Hydrophilic Interactions
dc.subject.mesh	Polyglactin 910
dc.subject.mesh	Shear Strength
dc.subject.mesh	Staphylococcus aureus
dc.subject.mesh	Vancomycin
dc.subject.mesh	Staphylococcus aureus
dc.subject.mesh	Polyglactin 910
dc.subject.mesh	Vancomycin
dc.subject.mesh	Capsules
dc.subject.mesh	Anti-Bacterial Agents
dc.subject.mesh	Drug Compounding
dc.subject.mesh	Cell Survival
dc.subject.mesh	Diffusion
dc.subject.mesh	Fractional Precipitation
dc.subject.mesh	Compressive Strength
dc.subject.mesh	Shear Strength
dc.subject.mesh	Hydrophobic and Hydrophilic Interactions
dc.title	A reformative shear precipitation procedure for the fabrication of vancomycin-loaded poly(lactide-co-glycolide) microspheres.
dc.type	Journal Article
utslib.citation.volume	31
utslib.location.activity	England
utslib.for	0903 Biomedical Engineering
utslib.for	0912 Materials Engineering
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2022-09-01T05:59:32Z
pubs.issue	7
pubs.publication-status	Published
pubs.volume	31
utslib.citation.issue	7

Abstract:

This study reports the encapsulation of vancomycin, as a model hydrophilic drug, into poly(lactide-co-glycolide) microspheres using a novel reformative shear precipitation procedure. In contrast to the external aqueous phase used in the conventional microencapsulation technique based on emulsion solvent evaporation/extraction, the reformative shear precipitation procedure explored in this study uses a shear medium composed of glycerol as the viscous medium and ethanol as polymer antisolvent, which is relatively immiscible with the hydrophilic drug. This limits drug diffusion and leads to rapid microsphere solidification, which allows a large proportion of the hydrophilic drug to be encapsulated within the microspheres. The influence of various processing parameters, including polymer concentration, volume ratio of ethanol to glycerol in the shear medium, volume of aqueous drug solution, initial drug loading, and injecting rate of the drug-polymer emulsion, on the encapsulation efficiency and characteristics of resulting microspheres were investigated. The morphology and release characteristics, as well as mechanical, in vitro and in vivo behaviour of vancomycin-loaded poly(lactide-co-glycolide) microspheres prepared using the novel procedure were also investigated. The results demonstrated that the reformative shear precipitation procedure could achieve the loading of hydrophilic drugs into poly(lactide-co-glycolide) microspheres with high encapsulation efficiency, and the success of the procedure was largely influenced by the volume ratio of ethanol to glycerol in the shear medium. Vancomycin-loaded poly(lactide-co-glycolide) microspheres prepared using this procedure demonstrated favourable mechanical characteristics, antibacterial activity, and in vivo degradation behaviour which suggested their suitability for use as a sustained delivery system.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/161193