A method for visualization and isolation of elastic fibres in annulus fibrosus of the disc.

Tavakoli, J; Costi, JJ

A method for visualization and isolation of elastic fibres in annulus fibrosus of the disc.

Tavakoli, J

Costi, JJ

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Publisher:: ELSEVIER
Publication Type:: Journal Article
Citation:: Mater Sci Eng C Mater Biol Appl, 2018, 93, pp. 299-304
Issue Date:: 2018-12-01

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Field	Value	Language
dc.contributor.author	Tavakoli, J https://orcid.org/0000-0002-0696-6530
dc.contributor.author	Costi, JJ
dc.date.accessioned	2022-09-04T22:18:33Z
dc.date.available	2018-08-03
dc.date.available	2022-09-04T22:18:33Z
dc.date.issued	2018-12-01
dc.identifier.citation	Mater Sci Eng C Mater Biol Appl, 2018, 93, pp. 299-304
dc.identifier.issn	0928-4931
dc.identifier.issn	1873-0191
dc.identifier.uri	http://hdl.handle.net/10453/161352
dc.description.abstract	A simple and cost effective protocol for visualization and isolation of the elastic fibres network in the annulus fibrosus (AF) of the disc is explained, to provide other researchers a method that can be applied in disc ultra-structural analysis, biomechanical assessment of elastic fibre and tissue engineered scaffold fabrication. This protocol is developed based on simultaneous sonication and alkali digestion of tissue that eliminates all matrix constituents except for elastic fibres, which is applicable for different species including human. Thin samples harvested from ovine, bovine, porcine and human, which are commonly used in disc research, were exposed to 0.5 M sodium hydroxide solution along with sonication (25 kHz) in distilled water for defined periods of time at room temperature. Post heat treatment removed collagen fibres via the gelatinization process, for visualization of elastic fibres.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	ELSEVIER
dc.relation.ispartof	Mater Sci Eng C Mater Biol Appl
dc.relation.isbasedon	10.1016/j.msec.2018.08.007
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0903 Biomedical Engineering, 0912 Materials Engineering
dc.subject.classification	Biomedical Engineering
dc.subject.mesh	Animals
dc.subject.mesh	Annulus Fibrosus
dc.subject.mesh	Cattle
dc.subject.mesh	Elastic Tissue
dc.subject.mesh	Humans
dc.subject.mesh	Sheep
dc.subject.mesh	Tissue Scaffolds
dc.subject.mesh	Elastic Tissue
dc.subject.mesh	Animals
dc.subject.mesh	Cattle
dc.subject.mesh	Sheep
dc.subject.mesh	Humans
dc.subject.mesh	Tissue Scaffolds
dc.subject.mesh	Annulus Fibrosus
dc.title	A method for visualization and isolation of elastic fibres in annulus fibrosus of the disc.
dc.type	Journal Article
utslib.citation.volume	93
utslib.location.activity	Netherlands
utslib.for	0903 Biomedical Engineering
utslib.for	0912 Materials Engineering
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	closed_access	*
dc.date.updated	2022-09-04T22:18:32Z
pubs.publication-status	Published
pubs.volume	93

Abstract:

A simple and cost effective protocol for visualization and isolation of the elastic fibres network in the annulus fibrosus (AF) of the disc is explained, to provide other researchers a method that can be applied in disc ultra-structural analysis, biomechanical assessment of elastic fibre and tissue engineered scaffold fabrication. This protocol is developed based on simultaneous sonication and alkali digestion of tissue that eliminates all matrix constituents except for elastic fibres, which is applicable for different species including human. Thin samples harvested from ovine, bovine, porcine and human, which are commonly used in disc research, were exposed to 0.5 M sodium hydroxide solution along with sonication (25 kHz) in distilled water for defined periods of time at room temperature. Post heat treatment removed collagen fibres via the gelatinization process, for visualization of elastic fibres.

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http://hdl.handle.net/10453/161352