Novel Bacterial Cellulose/Gelatin Hydrogels as 3D Scaffolds for Tumor Cell Culture.

Wang, J; Zhao, L; Zhang, A; Huang, Y; Tavakoli, J; Tang, Y

Novel Bacterial Cellulose/Gelatin Hydrogels as 3D Scaffolds for Tumor Cell Culture.

Wang, J Zhao, L Zhang, A Huang, Y Tavakoli, J

Tang, Y

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Publisher:: MDPI
Publication Type:: Journal Article
Citation:: Polymers (Basel), 2018, 10, (6), pp. E581
Issue Date:: 2018-05-24

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Field	Value	Language
dc.contributor.author	Wang, J
dc.contributor.author	Zhao, L
dc.contributor.author	Zhang, A
dc.contributor.author	Huang, Y
dc.contributor.author	Tavakoli, J https://orcid.org/0000-0002-0696-6530
dc.contributor.author	Tang, Y
dc.date.accessioned	2022-09-05T20:52:15Z
dc.date.available	2018-05-22
dc.date.available	2022-09-05T20:52:15Z
dc.date.issued	2018-05-24
dc.identifier.citation	Polymers (Basel), 2018, 10, (6), pp. E581
dc.identifier.issn	2073-4360
dc.identifier.issn	2073-4360
dc.identifier.uri	http://hdl.handle.net/10453/161375
dc.description.abstract	Three-dimensional (3D) cells in vitro culture are becoming increasingly popular in cancer research because some important signals are lost when cells are cultured in a two-dimensional (2D) substrate. In this work, bacterial cellulose (BC)/gelatin hydrogels were successfully synthesized and were investigated as scaffolds for cancer cells in vitro culture to simulate tumor microenvironment. Their properties and ability to support normal growth of cancer cells were evaluated. In particular, the human breast cancer cell line (MDA-MD-231) was seeded into BC/gelatin scaffolds to investigate their potential in 3D cell in vitro culture. MTT proliferation assay, scanning electron microscopy, hematoxylin and eosin staining and immunofluorescence were used to determine cell proliferation, morphology, adhesion, infiltration, and receptor expression. The in vitro MDA-MD-231 cell culture results demonstrated that cells cultured on the BC/gelatin scaffolds had significant adhesion, proliferation, ingrowth and differentiation. More importantly, MDA-MD-231 cells cultured in BC/gelatin scaffolds retained triple-negative receptor expression, demonstrating that BC/gelatin scaffolds could be used as ideal in vitro culture scaffolds for tumor cells.
dc.format	Electronic
dc.language	eng
dc.publisher	MDPI
dc.relation.ispartof	Polymers (Basel)
dc.relation.isbasedon	10.3390/polym10060581
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	03 Chemical Sciences, 09 Engineering
dc.title	Novel Bacterial Cellulose/Gelatin Hydrogels as 3D Scaffolds for Tumor Cell Culture.
dc.type	Journal Article
utslib.citation.volume	10
utslib.location.activity	Switzerland
utslib.for	03 Chemical Sciences
utslib.for	09 Engineering
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	open_access	*
dc.date.updated	2022-09-05T20:52:10Z
pubs.issue	6
pubs.publication-status	Published online
pubs.volume	10
utslib.citation.issue	6

Abstract:

Three-dimensional (3D) cells in vitro culture are becoming increasingly popular in cancer research because some important signals are lost when cells are cultured in a two-dimensional (2D) substrate. In this work, bacterial cellulose (BC)/gelatin hydrogels were successfully synthesized and were investigated as scaffolds for cancer cells in vitro culture to simulate tumor microenvironment. Their properties and ability to support normal growth of cancer cells were evaluated. In particular, the human breast cancer cell line (MDA-MD-231) was seeded into BC/gelatin scaffolds to investigate their potential in 3D cell in vitro culture. MTT proliferation assay, scanning electron microscopy, hematoxylin and eosin staining and immunofluorescence were used to determine cell proliferation, morphology, adhesion, infiltration, and receptor expression. The in vitro MDA-MD-231 cell culture results demonstrated that cells cultured on the BC/gelatin scaffolds had significant adhesion, proliferation, ingrowth and differentiation. More importantly, MDA-MD-231 cells cultured in BC/gelatin scaffolds retained triple-negative receptor expression, demonstrating that BC/gelatin scaffolds could be used as ideal in vitro culture scaffolds for tumor cells.

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http://hdl.handle.net/10453/161375