X-ray radiation-induced and targeted photodynamic therapy with folic acid-conjugated biodegradable nanoconstructs.
- Publisher:
- DOVE MEDICAL PRESS LTD
- Publication Type:
- Journal Article
- Citation:
- Int J Nanomedicine, 2018, 13, pp. 3553-3570
- Issue Date:
- 2018
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Field | Value | Language |
---|---|---|
dc.contributor.author | Clement, S | |
dc.contributor.author | Chen, W | |
dc.contributor.author |
Deng, W https://orcid.org/0000-0002-9413-0978 |
|
dc.contributor.author | Goldys, EM | |
dc.date.accessioned | 2022-09-08T01:52:59Z | |
dc.date.available | 2022-09-08T01:52:59Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Int J Nanomedicine, 2018, 13, pp. 3553-3570 | |
dc.identifier.issn | 1176-9114 | |
dc.identifier.issn | 1178-2013 | |
dc.identifier.uri | http://hdl.handle.net/10453/161516 | |
dc.description.abstract | INTRODUCTION: The depth limitation of conventional photodynamic therapy (PDT) with visible electromagnetic radiation represents a challenge for the treatment of deep-seated tumors. MATERIALS AND METHODS: To overcome this issue, we developed an X-ray-induced PDT system where poly(lactide-co-glycolide) (PLGA) polymeric nanoparticles (NPs) incorporating a photosensitizer (PS), verteporfin (VP), were triggered by 6 MeV X-ray radiation to generate cytotoxic singlet oxygen. The X-ray radiation used in this study allows this system to breakthrough the PDT depth barrier due to excellent penetration of 6 MeV X-ray radiation through biological tissue. In addition, the conjugation of our NPs with folic acid moieties enables specific targeting of HCT116 cancer cells that overexpress the folate receptors. We carried out physiochemical characterization of PLGA NPs, such as size distribution, zeta potential, morphology and in vitro release of VP. Cellular uptake activity and cell-killing effect of these NPs were also evaluated. RESULTS AND DISCUSSION: Our results indicate that our nanoconstructs triggered by 6 MeV X-ray radiation yield enhanced PDT efficacy compared with the radiation alone. We attributed the X-ray-induced singlet oxygen generation from the PS, VP, to photoexcitation by Cherenkov radiation and/or reactive oxygen species generation facilitated by energetic secondary electrons produced in the tissue. CONCLUSION: The cytotoxic effect caused by VP offers the possibility of enhancing the radiation therapy commonly prescribed for the treatment of cancer by simultaneous PDT. | |
dc.format | Electronic-eCollection | |
dc.language | eng | |
dc.publisher | DOVE MEDICAL PRESS LTD | |
dc.relation | http://purl.org/au-research/grants/arc/CE140100003 | |
dc.relation.ispartof | Int J Nanomedicine | |
dc.relation.isbasedon | 10.2147/IJN.S164967 | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | 0601 Biochemistry and Cell Biology, 1007 Nanotechnology, 1115 Pharmacology and Pharmaceutical Sciences | |
dc.subject.classification | Nanoscience & Nanotechnology | |
dc.subject.mesh | Biocompatible Materials | |
dc.subject.mesh | Cell Line, Tumor | |
dc.subject.mesh | Cell Survival | |
dc.subject.mesh | Folic Acid | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Image Processing, Computer-Assisted | |
dc.subject.mesh | Lactic Acid | |
dc.subject.mesh | Nanoparticles | |
dc.subject.mesh | Particle Size | |
dc.subject.mesh | Photochemotherapy | |
dc.subject.mesh | Photosensitizing Agents | |
dc.subject.mesh | Polyglycolic Acid | |
dc.subject.mesh | Polylactic Acid-Polyglycolic Acid Copolymer | |
dc.subject.mesh | Porphyrins | |
dc.subject.mesh | Singlet Oxygen | |
dc.subject.mesh | Static Electricity | |
dc.subject.mesh | Verteporfin | |
dc.subject.mesh | X-Rays | |
dc.subject.mesh | Cell Line, Tumor | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Singlet Oxygen | |
dc.subject.mesh | Lactic Acid | |
dc.subject.mesh | Porphyrins | |
dc.subject.mesh | Folic Acid | |
dc.subject.mesh | Polyglycolic Acid | |
dc.subject.mesh | Photosensitizing Agents | |
dc.subject.mesh | Biocompatible Materials | |
dc.subject.mesh | Photochemotherapy | |
dc.subject.mesh | Cell Survival | |
dc.subject.mesh | Particle Size | |
dc.subject.mesh | X-Rays | |
dc.subject.mesh | Image Processing, Computer-Assisted | |
dc.subject.mesh | Nanoparticles | |
dc.subject.mesh | Static Electricity | |
dc.subject.mesh | Polylactic Acid-Polyglycolic Acid Copolymer | |
dc.subject.mesh | Verteporfin | |
dc.title | X-ray radiation-induced and targeted photodynamic therapy with folic acid-conjugated biodegradable nanoconstructs. | |
dc.type | Journal Article | |
utslib.citation.volume | 13 | |
utslib.location.activity | New Zealand | |
utslib.for | 0601 Biochemistry and Cell Biology | |
utslib.for | 1007 Nanotechnology | |
utslib.for | 1115 Pharmacology and Pharmaceutical Sciences | |
pubs.organisational-group | /University of Technology Sydney | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Engineering and Information Technology | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering | |
utslib.copyright.status | open_access | * |
dc.date.updated | 2022-09-08T01:52:53Z | |
pubs.publication-status | Published online | |
pubs.volume | 13 |
Abstract:
INTRODUCTION: The depth limitation of conventional photodynamic therapy (PDT) with visible electromagnetic radiation represents a challenge for the treatment of deep-seated tumors. MATERIALS AND METHODS: To overcome this issue, we developed an X-ray-induced PDT system where poly(lactide-co-glycolide) (PLGA) polymeric nanoparticles (NPs) incorporating a photosensitizer (PS), verteporfin (VP), were triggered by 6 MeV X-ray radiation to generate cytotoxic singlet oxygen. The X-ray radiation used in this study allows this system to breakthrough the PDT depth barrier due to excellent penetration of 6 MeV X-ray radiation through biological tissue. In addition, the conjugation of our NPs with folic acid moieties enables specific targeting of HCT116 cancer cells that overexpress the folate receptors. We carried out physiochemical characterization of PLGA NPs, such as size distribution, zeta potential, morphology and in vitro release of VP. Cellular uptake activity and cell-killing effect of these NPs were also evaluated. RESULTS AND DISCUSSION: Our results indicate that our nanoconstructs triggered by 6 MeV X-ray radiation yield enhanced PDT efficacy compared with the radiation alone. We attributed the X-ray-induced singlet oxygen generation from the PS, VP, to photoexcitation by Cherenkov radiation and/or reactive oxygen species generation facilitated by energetic secondary electrons produced in the tissue. CONCLUSION: The cytotoxic effect caused by VP offers the possibility of enhancing the radiation therapy commonly prescribed for the treatment of cancer by simultaneous PDT.
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