Reversible Cavitation-Induced Junctional Opening in an Artificial Endothelial Layer.

Silvani, G; Scognamiglio, C; Caprini, D; Marino, L; Chinappi, M; Sinibaldi, G; Peruzzi, G; Kiani, MF; Casciola, CM

Reversible Cavitation-Induced Junctional Opening in an Artificial Endothelial Layer.

Silvani, G

Scognamiglio, C Caprini, D Marino, L Chinappi, M Sinibaldi, G Peruzzi, G Kiani, MF Casciola, CM

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Publisher:: WILEY-V C H VERLAG GMBH
Publication Type:: Journal Article
Citation:: Small, 2019, 15, (51), pp. e1905375
Issue Date:: 2019-12

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Field	Value	Language
dc.contributor.author	Silvani, G https://orcid.org/0000-0001-6661-2817
dc.contributor.author	Scognamiglio, C
dc.contributor.author	Caprini, D
dc.contributor.author	Marino, L
dc.contributor.author	Chinappi, M
dc.contributor.author	Sinibaldi, G
dc.contributor.author	Peruzzi, G
dc.contributor.author	Kiani, MF
dc.contributor.author	Casciola, CM
dc.date.accessioned	2022-09-26T01:10:31Z
dc.date.available	2022-09-26T01:10:31Z
dc.date.issued	2019-12
dc.identifier.citation	Small, 2019, 15, (51), pp. e1905375
dc.identifier.issn	1613-6810
dc.identifier.issn	1613-6829
dc.identifier.uri	http://hdl.handle.net/10453/162059
dc.description.abstract	Targeting pharmaceuticals through the endothelial barrier is crucial for drug delivery. In this context, cavitation-assisted permeation shows promise for effective and reversible opening of intercellular junctions. A vessel-on-a-chip is exploited to investigate and quantify the effect of ultrasound-excited microbubbles-stable cavitation-on endothelial integrity. In the vessel-on-a-chip, the endothelial cells form a complete lumen under physiological shear stress, resulting in intercellular junctions that exhibit barrier functionality. Immunofluorescence microscopy is exploited to monitor vascular integrity following vascular endothelial cadherin staining. It is shown that microbubbles amplify the ultrasound effect, leading to the formation of interendothelial gaps that cause barrier permeabilization. The total gap area significantly increases with pressure amplitude compared to the control. Gap opening is fully reversible with gap area distribution returning to the control levels 45 min after insonication. The proposed integrated platform allows for precise and repeatable in vitro measurements of cavitation-enhanced endothelium permeability and shows potential for validating irradiation protocols for in vivo applications.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	WILEY-V C H VERLAG GMBH
dc.relation.ispartof	Small
dc.relation.isbasedon	10.1002/smll.201905375
dc.rights	info:eu-repo/semantics/openAccess
dc.subject.classification	Nanoscience & Nanotechnology
dc.subject.mesh	Endothelial Cells
dc.subject.mesh	Human Umbilical Vein Endothelial Cells
dc.subject.mesh	Humans
dc.subject.mesh	Microbubbles
dc.subject.mesh	Microscopy, Confocal
dc.subject.mesh	Microscopy, Fluorescence
dc.subject.mesh	Endothelial Cells
dc.subject.mesh	Humans
dc.subject.mesh	Microscopy, Confocal
dc.subject.mesh	Microscopy, Fluorescence
dc.subject.mesh	Microbubbles
dc.subject.mesh	Human Umbilical Vein Endothelial Cells
dc.title	Reversible Cavitation-Induced Junctional Opening in an Artificial Endothelial Layer.
dc.type	Journal Article
utslib.citation.volume	15
utslib.location.activity	Germany
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
utslib.copyright.status	open_access	*
dc.date.updated	2022-09-26T01:10:30Z
pubs.issue	51
pubs.publication-status	Published
pubs.volume	15
utslib.citation.issue	51

Abstract:

Targeting pharmaceuticals through the endothelial barrier is crucial for drug delivery. In this context, cavitation-assisted permeation shows promise for effective and reversible opening of intercellular junctions. A vessel-on-a-chip is exploited to investigate and quantify the effect of ultrasound-excited microbubbles-stable cavitation-on endothelial integrity. In the vessel-on-a-chip, the endothelial cells form a complete lumen under physiological shear stress, resulting in intercellular junctions that exhibit barrier functionality. Immunofluorescence microscopy is exploited to monitor vascular integrity following vascular endothelial cadherin staining. It is shown that microbubbles amplify the ultrasound effect, leading to the formation of interendothelial gaps that cause barrier permeabilization. The total gap area significantly increases with pressure amplitude compared to the control. Gap opening is fully reversible with gap area distribution returning to the control levels 45 min after insonication. The proposed integrated platform allows for precise and repeatable in vitro measurements of cavitation-enhanced endothelium permeability and shows potential for validating irradiation protocols for in vivo applications.

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http://hdl.handle.net/10453/162059