Meta-analysis reveals materiomic relationships in major ampullate silk across the spider phylogeny.

Craig, HC; Piorkowski, D; Nakagawa, S; Kasumovic, MM; Blamires, SJ

Meta-analysis reveals materiomic relationships in major ampullate silk across the spider phylogeny.

Craig, HC Piorkowski, D Nakagawa, S Kasumovic, MM Blamires, SJ

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Publisher:: ROYAL SOC
Publication Type:: Journal Article
Citation:: J R Soc Interface, 2020, 17, (170), pp. 20200471
Issue Date:: 2020-09

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Field	Value	Language
dc.contributor.author	Craig, HC
dc.contributor.author	Piorkowski, D
dc.contributor.author	Nakagawa, S
dc.contributor.author	Kasumovic, MM
dc.contributor.author	Blamires, SJ
dc.date.accessioned	2022-09-26T07:19:43Z
dc.date.available	2022-09-26T07:19:43Z
dc.date.issued	2020-09
dc.identifier.citation	J R Soc Interface, 2020, 17, (170), pp. 20200471
dc.identifier.issn	1742-5689
dc.identifier.issn	1742-5662
dc.identifier.uri	http://hdl.handle.net/10453/162094
dc.description.abstract	Spider major ampullate (MA) silk, with its combination of strength and extensibility, outperforms any synthetic equivalents. There is thus much interest in understanding its underlying materiome. While the expression of the different silk proteins (spidroins) appears an integral component of silk performance, our understanding of the nature of the relationship between the spidroins, their constituent amino acids and MA silk mechanics is ambiguous. To provide clarity on these relationships across spider species, we performed a meta-analysis using phylogenetic comparative methods. These showed that glycine and proline, both of which are indicators of differential spidroin expression, had effects on MA silk mechanics across the phylogeny. We also found serine to correlate with silk mechanics, probably via its presence within the carboxyl and amino-terminal domains of the spidroins. From our analyses, we concluded that the spidroin expression shifts across the phylogeny from predominantly MaSp1 in the MA silks of ancestral spiders to predominantly MaSp2 in the more derived spiders' silks. This trend was accompanied by an enhanced ultimate strain and decreased Young's modulus in the silks. Our meta-analysis enabled us to decipher between real and apparent influences on MA silk properties, providing significant insights into spider silk and web coevolution and enhancing our capacity to create spider silk-like materials.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	ROYAL SOC
dc.relation.ispartof	J R Soc Interface
dc.relation.isbasedon	10.1098/rsif.2020.0471
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject.classification	General Science & Technology
dc.subject.mesh	Amino Acids
dc.subject.mesh	Animals
dc.subject.mesh	Fibroins
dc.subject.mesh	Phylogeny
dc.subject.mesh	Silk
dc.subject.mesh	Spiders
dc.subject.mesh	Animals
dc.subject.mesh	Spiders
dc.subject.mesh	Silk
dc.subject.mesh	Amino Acids
dc.subject.mesh	Fibroins
dc.subject.mesh	Phylogeny
dc.title	Meta-analysis reveals materiomic relationships in major ampullate silk across the spider phylogeny.
dc.type	Journal Article
utslib.citation.volume	17
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Mechanical and Mechatronic Engineering
utslib.copyright.status	closed_access	*
dc.date.updated	2022-09-26T07:19:33Z
pubs.issue	170
pubs.publication-status	Published
pubs.volume	17
utslib.citation.issue	170

Abstract:

Spider major ampullate (MA) silk, with its combination of strength and extensibility, outperforms any synthetic equivalents. There is thus much interest in understanding its underlying materiome. While the expression of the different silk proteins (spidroins) appears an integral component of silk performance, our understanding of the nature of the relationship between the spidroins, their constituent amino acids and MA silk mechanics is ambiguous. To provide clarity on these relationships across spider species, we performed a meta-analysis using phylogenetic comparative methods. These showed that glycine and proline, both of which are indicators of differential spidroin expression, had effects on MA silk mechanics across the phylogeny. We also found serine to correlate with silk mechanics, probably via its presence within the carboxyl and amino-terminal domains of the spidroins. From our analyses, we concluded that the spidroin expression shifts across the phylogeny from predominantly MaSp1 in the MA silks of ancestral spiders to predominantly MaSp2 in the more derived spiders' silks. This trend was accompanied by an enhanced ultimate strain and decreased Young's modulus in the silks. Our meta-analysis enabled us to decipher between real and apparent influences on MA silk properties, providing significant insights into spider silk and web coevolution and enhancing our capacity to create spider silk-like materials.

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http://hdl.handle.net/10453/162094