Influencing Selectivity to Cancer Cells with Mixed Nanoparticles Prepared from Albumin-Polymer Conjugates and Block Copolymers
- Publisher:
- AMER CHEMICAL SOC
- Publication Type:
- Journal Article
- Citation:
- Bioconjugate Chemistry, 2017, 28, (4), pp. 979-985
- Issue Date:
- 2017
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| Filename | Description | Size | |||
|---|---|---|---|---|---|
| acs.bioconjchem.6b00698.pdf | Published version | 4.82 MB | Adobe PDF |
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Full metadata record
| Field | Value | Language |
|---|---|---|
| dc.contributor.author | Jiang, YY | |
| dc.contributor.author | Wong, S | |
| dc.contributor.author | Chen, F | |
| dc.contributor.author | Chang, T | |
| dc.contributor.author | Lu, HX | |
| dc.contributor.author | Stenzel, MH | |
| dc.date.accessioned | 2022-10-03T20:48:12Z | |
| dc.date.available | 2022-10-03T20:48:12Z | |
| dc.date.issued | 2017 | |
| dc.identifier.citation | Bioconjugate Chemistry, 2017, 28, (4), pp. 979-985 | |
| dc.identifier.issn | 1043-1802 | |
| dc.identifier.issn | 1520-4812 | |
| dc.identifier.uri | http://hdl.handle.net/10453/162244 | |
| dc.description.abstract | Albumin-based nanoparticles are widely used to delivery anticancer drug because they promote the accumulation of drugs in tumor sites. Nanoparticles with surface immobilized albumin are widely described in literature, although mixed nanoparticles with systematically modified ratios between albumin and PEG-based material are less common. In this work, hybrid nanoparticles were prepared by coassembly of a PEG-based amphiphilic block copolymer together with a polymer-protein conjugate. Poly(oligo(ethylene glycol) methyl ether acrylate)-poly(ε-caprolactone) (POEGMEA-PCL) was prepared by a combination of ring-opening polymerization and reversible addition-fragmentation chain transfer (RAFT) polymerization, while the polymer-protein conjugate was obtained by reacting poly(ε-caprolactone) with bovine serum albumin (BSA-PCL). Co-assembly of both amphiphiles at different ratios, with and without curcumin as a drug, led to hybrid nanoparticles with various amount of albumin on the particle surface. The resulting hybrid nanoparticles were similar in size (100-120 nm), but increasing the amount of albumin on the surface led to a more-negative ζ potential. The cytotoxicity of the curcumin-loaded nanoparticles was examined on several cell lines. The curcumin-loaded nanoparticles with high amount of albumin led to high cytotoxicity against breast cancer cell lines (MDA-MB-231 and MCF-7), which coincided with high cellular uptake. However, the cytotoxicity of the curcumin-loaded nanoparticles against CHO cells and RAW264.7 cells was reduced, suggesting that albumin can facilitate selectivity toward cancer cells. | |
| dc.format | Print-Electronic | |
| dc.language | eng | |
| dc.publisher | AMER CHEMICAL SOC | |
| dc.relation.ispartof | Bioconjugate Chemistry | |
| dc.relation.isbasedon | 10.1021/acs.bioconjchem.6b00698 | |
| dc.rights | info:eu-repo/semantics/closedAccess | |
| dc.subject | 0304 Medicinal and Biomolecular Chemistry, 0305 Organic Chemistry, 0601 Biochemistry and Cell Biology | |
| dc.subject.classification | Organic Chemistry | |
| dc.subject.mesh | Animals | |
| dc.subject.mesh | Antineoplastic Agents | |
| dc.subject.mesh | Breast | |
| dc.subject.mesh | Breast Neoplasms | |
| dc.subject.mesh | CHO Cells | |
| dc.subject.mesh | Cattle | |
| dc.subject.mesh | Cell Line, Tumor | |
| dc.subject.mesh | Cricetulus | |
| dc.subject.mesh | Curcumin | |
| dc.subject.mesh | Drug Carriers | |
| dc.subject.mesh | Female | |
| dc.subject.mesh | Humans | |
| dc.subject.mesh | Mice | |
| dc.subject.mesh | Models, Molecular | |
| dc.subject.mesh | Nanoparticles | |
| dc.subject.mesh | Polyesters | |
| dc.subject.mesh | Polyethylene Glycols | |
| dc.subject.mesh | RAW 264.7 Cells | |
| dc.subject.mesh | Serum Albumin, Bovine | |
| dc.subject.mesh | Breast | |
| dc.subject.mesh | Cell Line, Tumor | |
| dc.subject.mesh | CHO Cells | |
| dc.subject.mesh | Animals | |
| dc.subject.mesh | Cattle | |
| dc.subject.mesh | Humans | |
| dc.subject.mesh | Cricetulus | |
| dc.subject.mesh | Mice | |
| dc.subject.mesh | Breast Neoplasms | |
| dc.subject.mesh | Polyethylene Glycols | |
| dc.subject.mesh | Curcumin | |
| dc.subject.mesh | Polyesters | |
| dc.subject.mesh | Serum Albumin, Bovine | |
| dc.subject.mesh | Antineoplastic Agents | |
| dc.subject.mesh | Drug Carriers | |
| dc.subject.mesh | Models, Molecular | |
| dc.subject.mesh | Female | |
| dc.subject.mesh | Nanoparticles | |
| dc.subject.mesh | RAW 264.7 Cells | |
| dc.title | Influencing Selectivity to Cancer Cells with Mixed Nanoparticles Prepared from Albumin-Polymer Conjugates and Block Copolymers | |
| dc.type | Journal Article | |
| utslib.citation.volume | 28 | |
| utslib.location.activity | United States | |
| utslib.for | 0304 Medicinal and Biomolecular Chemistry | |
| utslib.for | 0305 Organic Chemistry | |
| utslib.for | 0601 Biochemistry and Cell Biology | |
| pubs.organisational-group | /University of Technology Sydney | |
| pubs.organisational-group | /University of Technology Sydney/Faculty of Engineering and Information Technology | |
| pubs.organisational-group | /University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering | |
| pubs.organisational-group | /University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices | |
| utslib.copyright.status | closed_access | * |
| dc.date.updated | 2022-10-03T20:48:08Z | |
| pubs.issue | 4 | |
| pubs.publication-status | Published | |
| pubs.volume | 28 | |
| utslib.citation.issue | 4 |
Abstract:
Albumin-based nanoparticles are widely used to delivery anticancer drug because they promote the accumulation of drugs in tumor sites. Nanoparticles with surface immobilized albumin are widely described in literature, although mixed nanoparticles with systematically modified ratios between albumin and PEG-based material are less common. In this work, hybrid nanoparticles were prepared by coassembly of a PEG-based amphiphilic block copolymer together with a polymer-protein conjugate. Poly(oligo(ethylene glycol) methyl ether acrylate)-poly(ε-caprolactone) (POEGMEA-PCL) was prepared by a combination of ring-opening polymerization and reversible addition-fragmentation chain transfer (RAFT) polymerization, while the polymer-protein conjugate was obtained by reacting poly(ε-caprolactone) with bovine serum albumin (BSA-PCL). Co-assembly of both amphiphiles at different ratios, with and without curcumin as a drug, led to hybrid nanoparticles with various amount of albumin on the particle surface. The resulting hybrid nanoparticles were similar in size (100-120 nm), but increasing the amount of albumin on the surface led to a more-negative ζ potential. The cytotoxicity of the curcumin-loaded nanoparticles was examined on several cell lines. The curcumin-loaded nanoparticles with high amount of albumin led to high cytotoxicity against breast cancer cell lines (MDA-MB-231 and MCF-7), which coincided with high cellular uptake. However, the cytotoxicity of the curcumin-loaded nanoparticles against CHO cells and RAW264.7 cells was reduced, suggesting that albumin can facilitate selectivity toward cancer cells.
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