Fluorescent Glyco Single-Chain Nanoparticle-Decorated Nanodiamonds

Wuest, KNR; Lu, H; Thomas, DS; Goldmann, AS; Stenzel, MH; Barner-Kowollik, C

Fluorescent Glyco Single-Chain Nanoparticle-Decorated Nanodiamonds

Wuest, KNR Lu, H

Thomas, DS Goldmann, AS Stenzel, MH Barner-Kowollik, C

Permalink

Publisher:: AMER CHEMICAL SOC
Publication Type:: Journal Article
Citation:: ACS Macro Letters, 2017, 6, (10), pp. 1168-1174
Issue Date:: 2017

Closed Access

	Filename	Description	Size
	acsmacrolett.7b00659.pdf	Published version	2.22 MB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Wuest, KNR
dc.contributor.author	Lu, H https://orcid.org/0000-0001-6932-1900
dc.contributor.author	Thomas, DS
dc.contributor.author	Goldmann, AS
dc.contributor.author	Stenzel, MH
dc.contributor.author	Barner-Kowollik, C
dc.date.accessioned	2022-10-03T20:53:59Z
dc.date.available	2022-10-03T20:53:59Z
dc.date.issued	2017
dc.identifier.citation	ACS Macro Letters, 2017, 6, (10), pp. 1168-1174
dc.identifier.issn	2161-1653
dc.identifier.issn	2161-1653
dc.identifier.uri	http://hdl.handle.net/10453/162248
dc.description.abstract	We introduce the light-induced collapse of single glycopolymer chains in water generating fluorescent glyco single-chain nanoparticles (SCNPs) and their subsequent functionalization onto nanodiamonds. The glycopolymer precursors are prepared by polymerizing an acetylated mannose-based methacrylate monomer followed by a deprotection and postpolymerization functionalization step, introducing profluorescent photoactive tetrazole groups and furan-protected maleimide moieties. Subsequent UV irradiation in highly diluted aqueous solution triggers intramolecular tetrazole-mediated cycloadditions, yielding glyco SCNPs featuring fluorescence as well as lectin binding properties. The obtained SCNPs are coated onto nanodiamonds by adsorption, and the obtained hybrid nanoparticles are in depth characterized in terms of size, functionality, and bioactivity. Different coating densities are achieved by altering the SCNP concentration. The prepared nanoparticles are nontoxic in mouse RAW 264.7 macrophages. Furthermore, the fluorescence of the SCNPs can be exploited to image the SCNP-coated nanodiamonds in macrophage cells via confocal fluorescence microscopy.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	AMER CHEMICAL SOC
dc.relation.ispartof	ACS Macro Letters
dc.relation.isbasedon	10.1021/acsmacrolett.7b00659
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0303 Macromolecular and Materials Chemistry, 0306 Physical Chemistry (incl. Structural)
dc.title	Fluorescent Glyco Single-Chain Nanoparticle-Decorated Nanodiamonds
dc.type	Journal Article
utslib.citation.volume	6
utslib.location.activity	United States
utslib.for	0303 Macromolecular and Materials Chemistry
utslib.for	0306 Physical Chemistry (incl. Structural)
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	closed_access	*
dc.date.updated	2022-10-03T20:53:57Z
pubs.issue	10
pubs.publication-status	Published
pubs.volume	6
utslib.citation.issue	10

Abstract:

We introduce the light-induced collapse of single glycopolymer chains in water generating fluorescent glyco single-chain nanoparticles (SCNPs) and their subsequent functionalization onto nanodiamonds. The glycopolymer precursors are prepared by polymerizing an acetylated mannose-based methacrylate monomer followed by a deprotection and postpolymerization functionalization step, introducing profluorescent photoactive tetrazole groups and furan-protected maleimide moieties. Subsequent UV irradiation in highly diluted aqueous solution triggers intramolecular tetrazole-mediated cycloadditions, yielding glyco SCNPs featuring fluorescence as well as lectin binding properties. The obtained SCNPs are coated onto nanodiamonds by adsorption, and the obtained hybrid nanoparticles are in depth characterized in terms of size, functionality, and bioactivity. Different coating densities are achieved by altering the SCNP concentration. The prepared nanoparticles are nontoxic in mouse RAW 264.7 macrophages. Furthermore, the fluorescence of the SCNPs can be exploited to image the SCNP-coated nanodiamonds in macrophage cells via confocal fluorescence microscopy.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/162248