Isolation and characterization of lactose and non-lactose fermenting bacteria from tertiary care hospital and their antimicrobial susceptibility test
- Publisher:
- Innovare Academic Sciences Pvt Ltd
- Publication Type:
- Journal Article
- Citation:
- Asian Journal of Pharmaceutical and Clinical Research, 2017, 10, (2), pp. 201-205
- Issue Date:
- 2017-02-01
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Objective: The purpose of the present study was to identify the fermenting and non-fermenting Gram-negative bacteria from the tertiary care hospital. Methods: The conventional method of identification by biochemical analysis and antibiotic susceptibility test was performed by Kirby-Bauer disc diffusion method. Furthermore, analysis of microbes was done by Vitek-2. Results: 424 strains of lactose fermenting Gram-negative bacilli (LFGNB) and non-LFGNB (NLFGNB) were isolated from 3097 clinical samples. From the total LF bacteria, Escherichia coli was the predominant isolate accounting for 50.94% specimens, followed by Klebsiella pneumoniae 27.59% and Enterobacter 0.47%. From the total NLFGNB, Acinetobacter baumannii was the predominant isolate accounting for 12.73% specimens followed by Pseudomonas aeroginosa 6.13%, other isolates were Stenotrophomonas maltophilia 1.17%, Burkholderia cepacia 0.94%. In the present study, male were more infected than female. The study also showed that LF bacteria were more infectious than non LF bacteria and isolates were from urine samples. Conclusion: Both NLFGNB and LFGNB Gram-negative bacilli were found to be major contaminants, and are important pathogenic bacteria causing a wide range of infections in the tertiary care hospital.
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