A thoroughgoing design of a rapid-cycle microfluidic droplet-based PCR device to amplify rare DNA strands

Mollajan, M; Razavi Bazaz, S; Abouei Mehrizi, A

A thoroughgoing design of a rapid-cycle microfluidic droplet-based PCR device to amplify rare DNA strands

Mollajan, M Razavi Bazaz, S

Abouei Mehrizi, A

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Publisher:: ISFAHAN UNIV TECHNOLOGY
Publication Type:: Journal Article
Citation:: Journal of Applied Fluid Mechanics, 2018, 11, (1), pp. 21-29
Issue Date:: 2018-01-01

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Field	Value	Language
dc.contributor.author	Mollajan, M
dc.contributor.author	Razavi Bazaz, S https://orcid.org/0000-0002-6419-3361
dc.contributor.author	Abouei Mehrizi, A
dc.date.accessioned	2022-10-29T00:19:12Z
dc.date.available	2022-10-29T00:19:12Z
dc.date.issued	2018-01-01
dc.identifier.citation	Journal of Applied Fluid Mechanics, 2018, 11, (1), pp. 21-29
dc.identifier.issn	1735-3572
dc.identifier.issn	1735-3645
dc.identifier.uri	http://hdl.handle.net/10453/162844
dc.description.abstract	DNA is a molecule and assortment of fruitful information of organisms and a wide range of viruses. Polymerase chain reaction (PCR) is a process used to amplify DNA strands in order to generate millions of them and extract the applicable information. Although conventional methods for PCR are flourishing to a certain extent, they have such major drawbacks as contamination, high material consumption, and low-speed function. By the combination of PCR devices with the microfluidic approach and integrating them with droplet generation technology, the mentioned problems can be eliminated. In this study, a novel two-step rapid-cycle dropletbased PCR (dPCR) device, considering the design of microchannel and heat transfer system, has been presented. First, numerous studies have been conducted to select the proper droplet generator for the integration of the droplet generation with the PCR device. Then, with the careful attention to the requirements of a PCR device, the geometry of different zones of the PCR device has been, meticulously, designed. In the next and last step, the heat transfer system for the designed zones of the PCR device has been planned. Afterward, results are examined carefully which indicate that in a cycle of PCR, they are not any major discrepancies between the designed dPCR and the ideal one-the one that is intended to be created.
dc.language	English
dc.publisher	ISFAHAN UNIV TECHNOLOGY
dc.relation.ispartof	Journal of Applied Fluid Mechanics
dc.relation.isbasedon	10.29252/jafm.11.01.28110
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0901 Aerospace Engineering, 0907 Environmental Engineering, 0915 Interdisciplinary Engineering
dc.title	A thoroughgoing design of a rapid-cycle microfluidic droplet-based PCR device to amplify rare DNA strands
dc.type	Journal Article
utslib.citation.volume	11
utslib.for	0901 Aerospace Engineering
utslib.for	0907 Environmental Engineering
utslib.for	0915 Interdisciplinary Engineering
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
utslib.copyright.status	closed_access	*
dc.date.updated	2022-10-29T00:19:10Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	11
utslib.citation.issue	1

Abstract:

DNA is a molecule and assortment of fruitful information of organisms and a wide range of viruses. Polymerase chain reaction (PCR) is a process used to amplify DNA strands in order to generate millions of them and extract the applicable information. Although conventional methods for PCR are flourishing to a certain extent, they have such major drawbacks as contamination, high material consumption, and low-speed function. By the combination of PCR devices with the microfluidic approach and integrating them with droplet generation technology, the mentioned problems can be eliminated. In this study, a novel two-step rapid-cycle dropletbased PCR (dPCR) device, considering the design of microchannel and heat transfer system, has been presented. First, numerous studies have been conducted to select the proper droplet generator for the integration of the droplet generation with the PCR device. Then, with the careful attention to the requirements of a PCR device, the geometry of different zones of the PCR device has been, meticulously, designed. In the next and last step, the heat transfer system for the designed zones of the PCR device has been planned. Afterward, results are examined carefully which indicate that in a cycle of PCR, they are not any major discrepancies between the designed dPCR and the ideal one-the one that is intended to be created.

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http://hdl.handle.net/10453/162844