Size-controlled droplet generation in a microfluidic device for rare DNA amplification by optimizing its effective parameters

Lashkaripour, A; Mehrizi, AA; Goharimanesh, M; Rasouli, M; Bazaz, SR

Size-controlled droplet generation in a microfluidic device for rare DNA amplification by optimizing its effective parameters

Lashkaripour, A Mehrizi, AA Goharimanesh, M Rasouli, M Bazaz, SR

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Publisher:: WORLD SCIENTIFIC PUBL CO PTE LTD
Publication Type:: Journal Article
Citation:: Journal of Mechanics in Medicine and Biology, 2018, 18, (1)
Issue Date:: 2018-02-01

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Field	Value	Language
dc.contributor.author	Lashkaripour, A
dc.contributor.author	Mehrizi, AA
dc.contributor.author	Goharimanesh, M
dc.contributor.author	Rasouli, M
dc.contributor.author	Bazaz, SR
dc.date.accessioned	2022-10-29T00:36:17Z
dc.date.available	2022-10-29T00:36:17Z
dc.date.issued	2018-02-01
dc.identifier.citation	Journal of Mechanics in Medicine and Biology, 2018, 18, (1)
dc.identifier.issn	0219-5194
dc.identifier.issn	1793-6810
dc.identifier.uri	http://hdl.handle.net/10453/162846
dc.description.abstract	Versatility and portability of microfluidic devices play a dominant role in their widespread use by researchers. Droplet-based microfluidic devices have been extensively used due to their precise control over sample volume, and ease of manipulating and addressing each droplet on demand. Droplet-based polymerase chain reaction (PCR) devices are particularly desirable in single DNA amplification. If the droplets are small enough to contain only one DNA molecule, single molecule amplification becomes possible, which can be advantageous in several cases such as early cancer detection. In this work, flow-focusing microfluidic droplet generation's parameters are numerically investigated and optimized for generating the smallest droplet possible, while considering fabrication limits. Taguchi design of experiment method is used to study the effects of key parameters in droplet generation. By exploiting this approach, a droplet with a radius of 111nm is generated using a 3μm orifice. Since the governing physics of the droplet generation process is not totally understood yet, by means of analysis of variance (ANOVA) analysis, a generalized linear model (GLM) is proposed to predict the droplet radius, given the values of eight major parameters affecting the droplet size. The proposed model shows a correlation of 95.3% and 64.95% for droplets of radius greater than and lower than 5μm, respectively. Finally, the source of this variation of behavior in different size scales is identified.
dc.language	English
dc.publisher	WORLD SCIENTIFIC PUBL CO PTE LTD
dc.relation.ispartof	Journal of Mechanics in Medicine and Biology
dc.relation.isbasedon	10.1142/S0219519418500021
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0903 Biomedical Engineering, 0912 Materials Engineering
dc.title	Size-controlled droplet generation in a microfluidic device for rare DNA amplification by optimizing its effective parameters
dc.type	Journal Article
utslib.citation.volume	18
utslib.for	0903 Biomedical Engineering
utslib.for	0912 Materials Engineering
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
utslib.copyright.status	closed_access	*
dc.date.updated	2022-10-29T00:36:15Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	18
utslib.citation.issue	1

Abstract:

Versatility and portability of microfluidic devices play a dominant role in their widespread use by researchers. Droplet-based microfluidic devices have been extensively used due to their precise control over sample volume, and ease of manipulating and addressing each droplet on demand. Droplet-based polymerase chain reaction (PCR) devices are particularly desirable in single DNA amplification. If the droplets are small enough to contain only one DNA molecule, single molecule amplification becomes possible, which can be advantageous in several cases such as early cancer detection. In this work, flow-focusing microfluidic droplet generation's parameters are numerically investigated and optimized for generating the smallest droplet possible, while considering fabrication limits. Taguchi design of experiment method is used to study the effects of key parameters in droplet generation. By exploiting this approach, a droplet with a radius of 111nm is generated using a 3μm orifice. Since the governing physics of the droplet generation process is not totally understood yet, by means of analysis of variance (ANOVA) analysis, a generalized linear model (GLM) is proposed to predict the droplet radius, given the values of eight major parameters affecting the droplet size. The proposed model shows a correlation of 95.3% and 64.95% for droplets of radius greater than and lower than 5μm, respectively. Finally, the source of this variation of behavior in different size scales is identified.

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http://hdl.handle.net/10453/162846