A biphasic scaffold based on silk and bioactive ceramic with stratified properties for osteochondral tissue regeneration.

Li, JJ; Kim, K; Roohani-Esfahani, S-I; Guo, J; Kaplan, DL; Zreiqat, H

A biphasic scaffold based on silk and bioactive ceramic with stratified properties for osteochondral tissue regeneration.

Li, JJ Kim, K Roohani-Esfahani, S-I Guo, J Kaplan, DL Zreiqat, H

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Publisher:: ROYAL SOC CHEMISTRY
Publication Type:: Journal Article
Citation:: J Mater Chem B, 2015, 3, (26), pp. 5361-5376
Issue Date:: 2015-07-14

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Field	Value	Language
dc.contributor.author	Li, JJ
dc.contributor.author	Kim, K
dc.contributor.author	Roohani-Esfahani, S-I
dc.contributor.author	Guo, J
dc.contributor.author	Kaplan, DL
dc.contributor.author	Zreiqat, H
dc.date.accessioned	2023-01-18T05:33:00Z
dc.date.available	2023-01-18T05:33:00Z
dc.date.issued	2015-07-14
dc.identifier.citation	J Mater Chem B, 2015, 3, (26), pp. 5361-5376
dc.identifier.issn	2050-750X
dc.identifier.issn	2050-7518
dc.identifier.uri	http://hdl.handle.net/10453/165139
dc.description.abstract	Significant clinical challenges encountered in the effective long-term treatment of osteochondral defects have inspired advancements in scaffold-based tissue engineering techniques to aid repair and regeneration. This study reports the development of a biphasic scaffold produced via a rational combination of silk fibroin and bioactive ceramic with stratified properties to satisfy the complex and diverse regenerative requirements of osteochondral tissue. Structural examination showed that the biphasic scaffold contained two phases with different pore morphologies to match the cartilage and bone segments of osteochondral tissue, which were joined at a continuous interface. Mechanical assessment showed that the two phases of the biphasic scaffold imitated the load-bearing behaviour of native osteochondral tissue and matched its compressive properties. In vitro testing showed that different compositions in the two phases of the biphasic scaffold could direct the preferential differentiation of human mesenchymal stem cells towards the chondrogenic or osteogenic lineage. By featuring simple and reproducible fabrication and a well-integrated interface, the biphasic scaffold strategy established in this study circumvented the common problems experienced with integrated scaffold designs and could provide an effective approach for the regeneration of osteochondral tissue.
dc.format	Print
dc.language	eng
dc.publisher	ROYAL SOC CHEMISTRY
dc.relation.ispartof	J Mater Chem B
dc.relation.isbasedon	10.1039/C5TB00353A
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0303 Macromolecular and Materials Chemistry, 0903 Biomedical Engineering
dc.title	A biphasic scaffold based on silk and bioactive ceramic with stratified properties for osteochondral tissue regeneration.
dc.type	Journal Article
utslib.citation.volume	3
utslib.location.activity	England
utslib.for	0303 Macromolecular and Materials Chemistry
utslib.for	0903 Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
utslib.copyright.status	closed_access	*
dc.date.updated	2023-01-18T05:32:55Z
pubs.issue	26
pubs.publication-status	Published
pubs.volume	3
utslib.citation.issue	26

Abstract:

Significant clinical challenges encountered in the effective long-term treatment of osteochondral defects have inspired advancements in scaffold-based tissue engineering techniques to aid repair and regeneration. This study reports the development of a biphasic scaffold produced via a rational combination of silk fibroin and bioactive ceramic with stratified properties to satisfy the complex and diverse regenerative requirements of osteochondral tissue. Structural examination showed that the biphasic scaffold contained two phases with different pore morphologies to match the cartilage and bone segments of osteochondral tissue, which were joined at a continuous interface. Mechanical assessment showed that the two phases of the biphasic scaffold imitated the load-bearing behaviour of native osteochondral tissue and matched its compressive properties. In vitro testing showed that different compositions in the two phases of the biphasic scaffold could direct the preferential differentiation of human mesenchymal stem cells towards the chondrogenic or osteogenic lineage. By featuring simple and reproducible fabrication and a well-integrated interface, the biphasic scaffold strategy established in this study circumvented the common problems experienced with integrated scaffold designs and could provide an effective approach for the regeneration of osteochondral tissue.

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http://hdl.handle.net/10453/165139