Single Small Extracellular Vesicle (sEV) Quantification by Upconversion Nanoparticles.

Huang, G; Zhu, Y; Wen, S; Mei, H; Liu, Y; Wang, D; Maddahfar, M; Su, QP; Lin, G; Chen, Y; Jin, D

Single Small Extracellular Vesicle (sEV) Quantification by Upconversion Nanoparticles.

Huang, G

Zhu, Y

Wen, S

Mei, H Liu, Y Wang, D Maddahfar, M Su, QP Lin, G

Chen, Y

Jin, D

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Publisher:: AMER CHEMICAL SOC
Publication Type:: Journal Article
Citation:: Nano Lett, 2022, 22, (9), pp. 3761-3769
Issue Date:: 2022-05-11

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Field	Value	Language
dc.contributor.author	Huang, G https://orcid.org/0000-0001-7524-8199
dc.contributor.author	Zhu, Y https://orcid.org/0000-0002-8840-176X
dc.contributor.author	Wen, S https://orcid.org/0000-0002-4670-4658
dc.contributor.author	Mei, H
dc.contributor.author	Liu, Y
dc.contributor.author	Wang, D
dc.contributor.author	Maddahfar, M
dc.contributor.author	Su, QP
dc.contributor.author	Lin, G https://orcid.org/0000-0001-9880-8478
dc.contributor.author	Chen, Y https://orcid.org/0000-0002-7886-7144
dc.contributor.author	Jin, D https://orcid.org/0000-0003-1046-2666
dc.date.accessioned	2023-01-30T04:32:39Z
dc.date.available	2023-01-30T04:32:39Z
dc.date.issued	2022-05-11
dc.identifier.citation	Nano Lett, 2022, 22, (9), pp. 3761-3769
dc.identifier.issn	1530-6984
dc.identifier.issn	1530-6992
dc.identifier.uri	http://hdl.handle.net/10453/165589
dc.description.abstract	Cancer-derived small extracellular vesicles (sEVs) are potential circulating biomarkers in liquid biopsies. However, their small sizes, low abundance, and heterogeneity in molecular makeups pose major technical challenges for detecting and characterizing them quantitatively. Here, we demonstrate a single-sEV enumeration platform using lanthanide-doped upconversion nanoparticles (UCNPs). Taking advantage of the unique optical properties of UCNPs and the background-eliminating property of total internal reflection fluorescence (TIRF) imaging technique, a single-sEV assay recorded a limit of detection 1.8 × 106 EVs/mL, which was nearly 3 orders of magnitude lower than the standard enzyme-linked immunosorbent assay (ELISA). Its specificity was validated by the difference between EpCAM-positive and EpCAM-negative sEVs. The accuracy of the UCNP-based single-sEV assay was benchmarked with immunomagnetic-beads flow cytometry, showing a high correlation (R2> 0.99). The platform is suitable for evaluating the heterogeneous antigen expression of sEV and can be easily adapted for biomarker discoveries and disease diagnosis.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	AMER CHEMICAL SOC
dc.relation	http://purl.org/au-research/grants/nhmrc/1160635
dc.relation	National Natural Science Foundation of China61729501
dc.relation	http://purl.org/au-research/grants/arc/DE220100846
dc.relation	http://purl.org/au-research/grants/arc/FL210100180
dc.relation.ispartof	Nano Lett
dc.relation.isbasedon	10.1021/acs.nanolett.2c00724
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject.classification	Nanoscience & Nanotechnology
dc.subject.mesh	Epithelial Cell Adhesion Molecule
dc.subject.mesh	Extracellular Vesicles
dc.subject.mesh	Humans
dc.subject.mesh	Lanthanoid Series Elements
dc.subject.mesh	Nanoparticles
dc.subject.mesh	Neoplasms
dc.subject.mesh	Humans
dc.subject.mesh	Neoplasms
dc.subject.mesh	Lanthanoid Series Elements
dc.subject.mesh	Nanoparticles
dc.subject.mesh	Extracellular Vesicles
dc.subject.mesh	Epithelial Cell Adhesion Molecule
dc.title	Single Small Extracellular Vesicle (sEV) Quantification by Upconversion Nanoparticles.
dc.type	Journal Article
utslib.citation.volume	22
utslib.location.activity	United States
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	closed_access	*
dc.date.updated	2023-01-30T04:32:38Z
pubs.issue	9
pubs.publication-status	Published
pubs.volume	22
utslib.citation.issue	9

Abstract:

Cancer-derived small extracellular vesicles (sEVs) are potential circulating biomarkers in liquid biopsies. However, their small sizes, low abundance, and heterogeneity in molecular makeups pose major technical challenges for detecting and characterizing them quantitatively. Here, we demonstrate a single-sEV enumeration platform using lanthanide-doped upconversion nanoparticles (UCNPs). Taking advantage of the unique optical properties of UCNPs and the background-eliminating property of total internal reflection fluorescence (TIRF) imaging technique, a single-sEV assay recorded a limit of detection 1.8 × 106 EVs/mL, which was nearly 3 orders of magnitude lower than the standard enzyme-linked immunosorbent assay (ELISA). Its specificity was validated by the difference between EpCAM-positive and EpCAM-negative sEVs. The accuracy of the UCNP-based single-sEV assay was benchmarked with immunomagnetic-beads flow cytometry, showing a high correlation (R2> 0.99). The platform is suitable for evaluating the heterogeneous antigen expression of sEV and can be easily adapted for biomarker discoveries and disease diagnosis.

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http://hdl.handle.net/10453/165589