Progesterone drives mammary secretory differentiation via RankL-mediated induction of Elf5 in luminal progenitor cells.

Lee, HJ; Gallego-Ortega, D; Ledger, A; Schramek, D; Joshi, P; Szwarc, MM; Cho, C; Lydon, JP; Khokha, R; Penninger, JM; Ormandy, CJ

Progesterone drives mammary secretory differentiation via RankL-mediated induction of Elf5 in luminal progenitor cells.

Lee, HJ Gallego-Ortega, D Ledger, A Schramek, D Joshi, P Szwarc, MM Cho, C Lydon, JP Khokha, R Penninger, JM Ormandy, CJ

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Publisher:: COMPANY BIOLOGISTS LTD
Publication Type:: Journal Article
Citation:: Development, 2013, 140, (7), pp. 1397-1401
Issue Date:: 2013-04

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Field	Value	Language
dc.contributor.author	Lee, HJ
dc.contributor.author	Gallego-Ortega, D
dc.contributor.author	Ledger, A
dc.contributor.author	Schramek, D
dc.contributor.author	Joshi, P
dc.contributor.author	Szwarc, MM
dc.contributor.author	Cho, C
dc.contributor.author	Lydon, JP
dc.contributor.author	Khokha, R
dc.contributor.author	Penninger, JM
dc.contributor.author	Ormandy, CJ
dc.date.accessioned	2023-02-27T19:33:44Z
dc.date.available	2023-02-27T19:33:44Z
dc.date.issued	2013-04
dc.identifier.citation	Development, 2013, 140, (7), pp. 1397-1401
dc.identifier.issn	0950-1991
dc.identifier.issn	1477-9129
dc.identifier.uri	http://hdl.handle.net/10453/166472
dc.description.abstract	Progesterone-RankL paracrine signaling has been proposed as a driver of stem cell expansion in the mammary gland, and Elf5 is essential for the differentiation of mammary epithelial progenitor cells. We demonstrate that Elf5 expression is induced by progesterone and that Elf5 and progesterone cooperate to promote alveolar development. The progesterone receptor and Elf5 are expressed in a mutually exclusive pattern, and we identify RankL as the paracrine mediator of the effects of progesterone on Elf5 expression in CD61+ progenitor cells and their consequent differentiation. Blockade of RankL action prevented progesterone-induced side branching and the expansion of Elf5(+) mature luminal cells. These findings describe a mechanism by which steroid hormones can produce the expansion of steroid hormone receptor-negative mammary epithelial cells.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	COMPANY BIOLOGISTS LTD
dc.relation.ispartof	Development
dc.relation.isbasedon	10.1242/dev.088948
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	06 Biological Sciences, 11 Medical and Health Sciences
dc.subject.mesh	Animals
dc.subject.mesh	DNA-Binding Proteins
dc.subject.mesh	Epithelial Cells
dc.subject.mesh	Female
dc.subject.mesh	Gene Expression Regulation, Developmental
dc.subject.mesh	Mammary Glands, Animal
dc.subject.mesh	Mice
dc.subject.mesh	Mice, Transgenic
dc.subject.mesh	Progesterone
dc.subject.mesh	RANK Ligand
dc.subject.mesh	Receptors, Progesterone
dc.subject.mesh	Stem Cells
dc.subject.mesh	Transcription Factors
dc.subject.mesh	Up-Regulation
dc.subject.mesh	Mammary Glands, Animal
dc.subject.mesh	Epithelial Cells
dc.subject.mesh	Stem Cells
dc.subject.mesh	Animals
dc.subject.mesh	Mice, Transgenic
dc.subject.mesh	Mice
dc.subject.mesh	Progesterone
dc.subject.mesh	DNA-Binding Proteins
dc.subject.mesh	Receptors, Progesterone
dc.subject.mesh	Transcription Factors
dc.subject.mesh	Gene Expression Regulation, Developmental
dc.subject.mesh	Up-Regulation
dc.subject.mesh	Female
dc.subject.mesh	RANK Ligand
dc.title	Progesterone drives mammary secretory differentiation via RankL-mediated induction of Elf5 in luminal progenitor cells.
dc.type	Journal Article
utslib.citation.volume	140
utslib.location.activity	England
utslib.for	06 Biological Sciences
utslib.for	11 Medical and Health Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	closed_access	*
dc.date.updated	2023-02-27T19:33:42Z
pubs.issue	7
pubs.publication-status	Published
pubs.volume	140
utslib.citation.issue	7

Abstract:

Progesterone-RankL paracrine signaling has been proposed as a driver of stem cell expansion in the mammary gland, and Elf5 is essential for the differentiation of mammary epithelial progenitor cells. We demonstrate that Elf5 expression is induced by progesterone and that Elf5 and progesterone cooperate to promote alveolar development. The progesterone receptor and Elf5 are expressed in a mutually exclusive pattern, and we identify RankL as the paracrine mediator of the effects of progesterone on Elf5 expression in CD61+ progenitor cells and their consequent differentiation. Blockade of RankL action prevented progesterone-induced side branching and the expansion of Elf5(+) mature luminal cells. These findings describe a mechanism by which steroid hormones can produce the expansion of steroid hormone receptor-negative mammary epithelial cells.

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http://hdl.handle.net/10453/166472