In vitro modeling of myocardial ischemia/reperfusion injury with murine or human 3D cardiac spheroids.

Sharma, P; Liu Chung Ming, C; Gentile, C

In vitro modeling of myocardial ischemia/reperfusion injury with murine or human 3D cardiac spheroids.

Sharma, P Liu Chung Ming, C Gentile, C

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Publisher:: Elsevier
Publication Type:: Journal Article
Citation:: STAR Protoc, 2022, 3, (4), pp. 101751
Issue Date:: 2022-12-16

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Field	Value	Language
dc.contributor.author	Sharma, P
dc.contributor.author	Liu Chung Ming, C
dc.contributor.author	Gentile, C https://orcid.org/0000-0002-3689-4275
dc.date.accessioned	2023-02-28T05:40:23Z
dc.date.available	2022-09-14
dc.date.available	2023-02-28T05:40:23Z
dc.date.issued	2022-12-16
dc.identifier.citation	STAR Protoc, 2022, 3, (4), pp. 101751
dc.identifier.issn	2666-1667
dc.identifier.issn	2666-1667
dc.identifier.uri	http://hdl.handle.net/10453/166569
dc.description.abstract	Myocardial infarction (MI) is the primary cause of death worldwide, but there are no clinically relevant models to study MI. Here, we describe an ischemia/reperfusion (I/R) injury model typical of MI using mouse or human 3D in vitro cardiac spheroids (CSs). First, we demonstrated the culture and maintenance of CSs. Then, we detailed how to expose CSs to pathophysiological oxygen concentrations to induce I/R injury. The protocol can be used in combination with viability, contractility, and mRNA expression level measurements. For complete details on the use and execution of this protocol, please refer to Sharma et al. (2022).
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Elsevier
dc.relation.ispartof	STAR Protoc
dc.relation.isbasedon	10.1016/j.xpro.2022.101751
dc.rights	info:eu-repo/semantics/openAccess
dc.subject.mesh	Humans
dc.subject.mesh	Mice
dc.subject.mesh	Animals
dc.subject.mesh	Myocardial Reperfusion Injury
dc.subject.mesh	Disease Models, Animal
dc.subject.mesh	Heart
dc.subject.mesh	Myocardial Infarction
dc.subject.mesh	Heart
dc.subject.mesh	Animals
dc.subject.mesh	Humans
dc.subject.mesh	Mice
dc.subject.mesh	Myocardial Reperfusion Injury
dc.subject.mesh	Myocardial Infarction
dc.subject.mesh	Disease Models, Animal
dc.subject.mesh	Humans
dc.subject.mesh	Mice
dc.subject.mesh	Animals
dc.subject.mesh	Myocardial Reperfusion Injury
dc.subject.mesh	Disease Models, Animal
dc.subject.mesh	Heart
dc.subject.mesh	Myocardial Infarction
dc.title	In vitro modeling of myocardial ischemia/reperfusion injury with murine or human 3D cardiac spheroids.
dc.type	Journal Article
utslib.citation.volume	3
utslib.location.activity	United States
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	open_access	*
dc.date.updated	2023-02-28T05:39:11Z
pubs.issue	4
pubs.publication-status	Published
pubs.volume	3
utslib.citation.issue	4

Abstract:

Myocardial infarction (MI) is the primary cause of death worldwide, but there are no clinically relevant models to study MI. Here, we describe an ischemia/reperfusion (I/R) injury model typical of MI using mouse or human 3D in vitro cardiac spheroids (CSs). First, we demonstrated the culture and maintenance of CSs. Then, we detailed how to expose CSs to pathophysiological oxygen concentrations to induce I/R injury. The protocol can be used in combination with viability, contractility, and mRNA expression level measurements. For complete details on the use and execution of this protocol, please refer to Sharma et al. (2022).

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http://hdl.handle.net/10453/166569