Generation and Culture of Organotypic Breast Carcinoma Spheroids for the Study of Drug Response in a 3D Microfluidic Device.

Aboulkheyr Es, H; Aref, AR; Warkiani, ME

Generation and Culture of Organotypic Breast Carcinoma Spheroids for the Study of Drug Response in a 3D Microfluidic Device.

Aboulkheyr Es, H Aref, AR Warkiani, ME

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Publisher:: Humana Press
Publication Type:: Journal Article
Citation:: Methods in Molecular Biology, 2022, 2535, pp. 49-57
Issue Date:: 2022

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Field	Value	Language
dc.contributor.author	Aboulkheyr Es, H
dc.contributor.author	Aref, AR
dc.contributor.author	Warkiani, ME
dc.date.accessioned	2023-03-05T01:27:14Z
dc.date.available	2023-03-05T01:27:14Z
dc.date.issued	2022
dc.identifier.citation	Methods in Molecular Biology, 2022, 2535, pp. 49-57
dc.identifier.isbn	9781071625125
dc.identifier.issn	1064-3745
dc.identifier.issn	1940-6029
dc.identifier.uri	http://hdl.handle.net/10453/166664
dc.description.abstract	Breast cancer (BC) is a leading cause of cancer death among women worldwide. To better understand and predict therapeutic response in BC patient developing a fast, low-cost, and reliable preclinical tumor from patient's tumor specimen is needed. Here, we describe the development of a preclinical model of BC through the generation and ex vivo culture of patient-derived organotypic tumor spheroids (PDOTS) in a 3D microfluidic device. Moreover, the real-time screening of conventional chemotherapy agents on cultured PDOTS is also described.
dc.format	Print
dc.language	eng
dc.publisher	Humana Press
dc.relation	http://purl.org/au-research/grants/nhmrc/1143377
dc.relation.ispartof	Methods in Molecular Biology
dc.relation.isbasedon	10.1007/978-1-0716-2513-2_4
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0399 Other Chemical Sciences, 0601 Biochemistry and Cell Biology
dc.subject.classification	Developmental Biology
dc.subject.mesh	Breast Neoplasms
dc.subject.mesh	Cell Culture Techniques
dc.subject.mesh	Female
dc.subject.mesh	Humans
dc.subject.mesh	Lab-On-A-Chip Devices
dc.subject.mesh	Microfluidic Analytical Techniques
dc.subject.mesh	Spheroids, Cellular
dc.subject.mesh	Breast Neoplasms
dc.subject.mesh	Cell Culture Techniques
dc.subject.mesh	Female
dc.subject.mesh	Humans
dc.subject.mesh	Lab-On-A-Chip Devices
dc.subject.mesh	Microfluidic Analytical Techniques
dc.subject.mesh	Spheroids, Cellular
dc.subject.mesh	Spheroids, Cellular
dc.subject.mesh	Humans
dc.subject.mesh	Breast Neoplasms
dc.subject.mesh	Microfluidic Analytical Techniques
dc.subject.mesh	Cell Culture Techniques
dc.subject.mesh	Female
dc.subject.mesh	Lab-On-A-Chip Devices
dc.subject.mesh	Breast Neoplasms
dc.subject.mesh	Cell Culture Techniques
dc.subject.mesh	Female
dc.subject.mesh	Humans
dc.subject.mesh	Lab-On-A-Chip Devices
dc.subject.mesh	Microfluidic Analytical Techniques
dc.subject.mesh	Spheroids, Cellular
dc.title	Generation and Culture of Organotypic Breast Carcinoma Spheroids for the Study of Drug Response in a 3D Microfluidic Device.
dc.type	Journal Article
utslib.citation.volume	2535
utslib.location.activity	United States
utslib.for	0399 Other Chemical Sciences
utslib.for	0601 Biochemistry and Cell Biology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2023-03-05T01:27:13Z
pubs.publication-status	Published
pubs.volume	2535

Abstract:

Breast cancer (BC) is a leading cause of cancer death among women worldwide. To better understand and predict therapeutic response in BC patient developing a fast, low-cost, and reliable preclinical tumor from patient's tumor specimen is needed. Here, we describe the development of a preclinical model of BC through the generation and ex vivo culture of patient-derived organotypic tumor spheroids (PDOTS) in a 3D microfluidic device. Moreover, the real-time screening of conventional chemotherapy agents on cultured PDOTS is also described.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/166664