Lipid-polymer nanocarrier platform enables X-ray induced photodynamic therapy against human colorectal cancer cells.

Sang, R; Deng, F; Engel, A; Goldys, E; Deng, W

Lipid-polymer nanocarrier platform enables X-ray induced photodynamic therapy against human colorectal cancer cells.

Sang, R Deng, F Engel, A Goldys, E Deng, W

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Publisher:: Elsevier BV
Publication Type:: Journal Article
Citation:: Biomed Pharmacother, 2022, 155, pp. 113837
Issue Date:: 2022-11

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Field	Value	Language
dc.contributor.author	Sang, R
dc.contributor.author	Deng, F
dc.contributor.author	Engel, A
dc.contributor.author	Goldys, E
dc.contributor.author	Deng, W https://orcid.org/0000-0002-9413-0978
dc.date.accessioned	2023-03-10T09:22:19Z
dc.date.available	2022-10-06
dc.date.available	2023-03-10T09:22:19Z
dc.date.issued	2022-11
dc.identifier.citation	Biomed Pharmacother, 2022, 155, pp. 113837
dc.identifier.issn	0753-3322
dc.identifier.issn	1950-6007
dc.identifier.uri	http://hdl.handle.net/10453/166985
dc.description.abstract	In this study, we brought together X-ray induced photodynamic therapy (X-PDT) and chemo-drug (5-FU) for the treatment on colorectal cancer cells. This was achieved by developing a lipid-polymer hybrid nanoparticle delivery system (FA-LPNPs-VP-5-FU). It was prepared by incorporating a photosensitizer (verteporfin), chemotherapy drug (5-FU) and a targeting moiety (folic acid) into one platform. The average size of these nanoparticles was around 100 nm with low polydispersity. When exposed to clinical doses of 4 Gy X-ray radiation, FA-LPNPs-VP-5-FU generated sufficient amounts of reactive oxygen species, triggering the apoptosis and necrosis pathway of cancer cells. Our combined X-PDT and chemo-drug strategy was effective in inhibiting cancer cells' growth and proliferation. Cell cycle analyses revealed that our treatment induced G2/M and S phase arrest in HCT116 cells. Our results indicate that this combined treatment provides better antitumour effect in colorectal cancer cells than each of these modalities alone. This may offer a novel approach for effective colorectal cancer treatment with reduced off-target effect and drug toxicity.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Elsevier BV
dc.relation	http://purl.org/au-research/grants/arc/CE140100003
dc.relation	http://purl.org/au-research/grants/nhmrc/1181889
dc.relation.ispartof	Biomed Pharmacother
dc.relation.isbasedon	10.1016/j.biopha.2022.113837
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	1115 Pharmacology and Pharmaceutical Sciences
dc.subject.classification	Oncology & Carcinogenesis
dc.subject.mesh	Humans
dc.subject.mesh	Photochemotherapy
dc.subject.mesh	Photosensitizing Agents
dc.subject.mesh	Polymers
dc.subject.mesh	Verteporfin
dc.subject.mesh	X-Rays
dc.subject.mesh	Reactive Oxygen Species
dc.subject.mesh	Cell Line, Tumor
dc.subject.mesh	Nanoparticles
dc.subject.mesh	Antineoplastic Agents
dc.subject.mesh	Folic Acid
dc.subject.mesh	Lipids
dc.subject.mesh	Fluorouracil
dc.subject.mesh	Colorectal Neoplasms
dc.subject.mesh	Cell Line, Tumor
dc.subject.mesh	Humans
dc.subject.mesh	Colorectal Neoplasms
dc.subject.mesh	Reactive Oxygen Species
dc.subject.mesh	Fluorouracil
dc.subject.mesh	Folic Acid
dc.subject.mesh	Polymers
dc.subject.mesh	Lipids
dc.subject.mesh	Antineoplastic Agents
dc.subject.mesh	Photosensitizing Agents
dc.subject.mesh	Photochemotherapy
dc.subject.mesh	X-Rays
dc.subject.mesh	Nanoparticles
dc.subject.mesh	Verteporfin
dc.title	Lipid-polymer nanocarrier platform enables X-ray induced photodynamic therapy against human colorectal cancer cells.
dc.type	Journal Article
utslib.citation.volume	155
utslib.location.activity	France
utslib.for	1115 Pharmacology and Pharmaceutical Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
utslib.copyright.status	open_access	*
dc.date.updated	2023-03-10T09:22:02Z
pubs.publication-status	Published
pubs.volume	155

Abstract:

In this study, we brought together X-ray induced photodynamic therapy (X-PDT) and chemo-drug (5-FU) for the treatment on colorectal cancer cells. This was achieved by developing a lipid-polymer hybrid nanoparticle delivery system (FA-LPNPs-VP-5-FU). It was prepared by incorporating a photosensitizer (verteporfin), chemotherapy drug (5-FU) and a targeting moiety (folic acid) into one platform. The average size of these nanoparticles was around 100 nm with low polydispersity. When exposed to clinical doses of 4 Gy X-ray radiation, FA-LPNPs-VP-5-FU generated sufficient amounts of reactive oxygen species, triggering the apoptosis and necrosis pathway of cancer cells. Our combined X-PDT and chemo-drug strategy was effective in inhibiting cancer cells' growth and proliferation. Cell cycle analyses revealed that our treatment induced G2/M and S phase arrest in HCT116 cells. Our results indicate that this combined treatment provides better antitumour effect in colorectal cancer cells than each of these modalities alone. This may offer a novel approach for effective colorectal cancer treatment with reduced off-target effect and drug toxicity.

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http://hdl.handle.net/10453/166985