Isolation and retrieval of circulating tumor cells using centrifugal forces.
- Publisher:
- NATURE PUBLISHING GROUP
- Publication Type:
- Journal Article
- Citation:
- Sci Rep, 2013, 3, (1), pp. 1259
- Issue Date:
- 2013
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Full metadata record
Field | Value | Language |
---|---|---|
dc.contributor.author | Hou, HW | |
dc.contributor.author | Warkiani, ME | |
dc.contributor.author | Khoo, BL | |
dc.contributor.author | Li, ZR | |
dc.contributor.author | Soo, RA | |
dc.contributor.author | Tan, DS-W | |
dc.contributor.author | Lim, W-T | |
dc.contributor.author | Han, J | |
dc.contributor.author | Bhagat, AAS | |
dc.contributor.author | Lim, CT | |
dc.date.accessioned | 2023-03-12T23:04:32Z | |
dc.date.available | 2013-01-28 | |
dc.date.available | 2023-03-12T23:04:32Z | |
dc.date.issued | 2013 | |
dc.identifier.citation | Sci Rep, 2013, 3, (1), pp. 1259 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | http://hdl.handle.net/10453/167093 | |
dc.description.abstract | Presence and frequency of rare circulating tumor cells (CTCs) in bloodstreams of cancer patients are pivotal to early cancer detection and treatment monitoring. Here, we use a spiral microchannel with inherent centrifugal forces for continuous, size-based separation of CTCs from blood (Dean Flow Fractionation (DFF)) which facilitates easy coupling with conventional downstream biological assays. Device performance was optimized using cancer cell lines (> 85% recovery), followed by clinical validation with positive CTCs enumeration in all samples from patients with metastatic lung cancer (n = 20; 5-88 CTCs per mL). The presence of CD133⁺ cells, a phenotypic marker characteristic of stem-like behavior in lung cancer cells was also identified in the isolated subpopulation of CTCs. The spiral biochip identifies and addresses key challenges of the next generation CTCs isolation assay including antibody independent isolation, high sensitivity and throughput (3 mL/hr); and single-step retrieval of viable CTCs. | |
dc.format | Print-Electronic | |
dc.language | eng | |
dc.publisher | NATURE PUBLISHING GROUP | |
dc.relation.ispartof | Sci Rep | |
dc.relation.isbasedon | 10.1038/srep01259 | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject.mesh | AC133 Antigen | |
dc.subject.mesh | Antigens, CD | |
dc.subject.mesh | Blood Cells | |
dc.subject.mesh | Cell Line, Tumor | |
dc.subject.mesh | Cell Separation | |
dc.subject.mesh | Centrifugation | |
dc.subject.mesh | Glycoproteins | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Keratins | |
dc.subject.mesh | Leukocyte Common Antigens | |
dc.subject.mesh | Lung Neoplasms | |
dc.subject.mesh | MCF-7 Cells | |
dc.subject.mesh | Microfluidic Analytical Techniques | |
dc.subject.mesh | Neoplastic Cells, Circulating | |
dc.subject.mesh | Peptides | |
dc.subject.mesh | Blood Cells | |
dc.subject.mesh | Cell Line, Tumor | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Lung Neoplasms | |
dc.subject.mesh | Glycoproteins | |
dc.subject.mesh | Peptides | |
dc.subject.mesh | Antigens, CD | |
dc.subject.mesh | Centrifugation | |
dc.subject.mesh | Microfluidic Analytical Techniques | |
dc.subject.mesh | Cell Separation | |
dc.subject.mesh | Keratins | |
dc.subject.mesh | Neoplastic Cells, Circulating | |
dc.subject.mesh | MCF-7 Cells | |
dc.subject.mesh | AC133 Antigen | |
dc.subject.mesh | Leukocyte Common Antigens | |
dc.subject.mesh | AC133 Antigen | |
dc.subject.mesh | Antigens, CD | |
dc.subject.mesh | Blood Cells | |
dc.subject.mesh | Cell Line, Tumor | |
dc.subject.mesh | Cell Separation | |
dc.subject.mesh | Centrifugation | |
dc.subject.mesh | Glycoproteins | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Keratins | |
dc.subject.mesh | Leukocyte Common Antigens | |
dc.subject.mesh | Lung Neoplasms | |
dc.subject.mesh | MCF-7 Cells | |
dc.subject.mesh | Microfluidic Analytical Techniques | |
dc.subject.mesh | Neoplastic Cells, Circulating | |
dc.subject.mesh | Peptides | |
dc.title | Isolation and retrieval of circulating tumor cells using centrifugal forces. | |
dc.type | Journal Article | |
utslib.citation.volume | 3 | |
utslib.location.activity | England | |
pubs.organisational-group | /University of Technology Sydney | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Engineering and Information Technology | |
pubs.organisational-group | /University of Technology Sydney/Strength - CHT - Health Technologies | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering | |
pubs.organisational-group | /University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices | |
pubs.organisational-group | /University of Technology Sydney/Centre for Health Technologies (CHT) | |
utslib.copyright.status | open_access | * |
dc.date.updated | 2023-03-12T23:04:28Z | |
pubs.issue | 1 | |
pubs.publication-status | Published | |
pubs.volume | 3 | |
utslib.citation.issue | 1 |
Abstract:
Presence and frequency of rare circulating tumor cells (CTCs) in bloodstreams of cancer patients are pivotal to early cancer detection and treatment monitoring. Here, we use a spiral microchannel with inherent centrifugal forces for continuous, size-based separation of CTCs from blood (Dean Flow Fractionation (DFF)) which facilitates easy coupling with conventional downstream biological assays. Device performance was optimized using cancer cell lines (> 85% recovery), followed by clinical validation with positive CTCs enumeration in all samples from patients with metastatic lung cancer (n = 20; 5-88 CTCs per mL). The presence of CD133⁺ cells, a phenotypic marker characteristic of stem-like behavior in lung cancer cells was also identified in the isolated subpopulation of CTCs. The spiral biochip identifies and addresses key challenges of the next generation CTCs isolation assay including antibody independent isolation, high sensitivity and throughput (3 mL/hr); and single-step retrieval of viable CTCs.
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