Isolation and retrieval of circulating tumor cells using centrifugal forces.

Hou, HW; Warkiani, ME; Khoo, BL; Li, ZR; Soo, RA; Tan, DS-W; Lim, W-T; Han, J; Bhagat, AAS; Lim, CT

Isolation and retrieval of circulating tumor cells using centrifugal forces.

Hou, HW Warkiani, ME Khoo, BL Li, ZR Soo, RA Tan, DS-W Lim, W-T Han, J Bhagat, AAS Lim, CT

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Publisher:: NATURE PUBLISHING GROUP
Publication Type:: Journal Article
Citation:: Sci Rep, 2013, 3, (1), pp. 1259
Issue Date:: 2013

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Field	Value	Language
dc.contributor.author	Hou, HW
dc.contributor.author	Warkiani, ME
dc.contributor.author	Khoo, BL
dc.contributor.author	Li, ZR
dc.contributor.author	Soo, RA
dc.contributor.author	Tan, DS-W
dc.contributor.author	Lim, W-T
dc.contributor.author	Han, J
dc.contributor.author	Bhagat, AAS
dc.contributor.author	Lim, CT
dc.date.accessioned	2023-03-12T23:04:32Z
dc.date.available	2013-01-28
dc.date.available	2023-03-12T23:04:32Z
dc.date.issued	2013
dc.identifier.citation	Sci Rep, 2013, 3, (1), pp. 1259
dc.identifier.issn	2045-2322
dc.identifier.issn	2045-2322
dc.identifier.uri	http://hdl.handle.net/10453/167093
dc.description.abstract	Presence and frequency of rare circulating tumor cells (CTCs) in bloodstreams of cancer patients are pivotal to early cancer detection and treatment monitoring. Here, we use a spiral microchannel with inherent centrifugal forces for continuous, size-based separation of CTCs from blood (Dean Flow Fractionation (DFF)) which facilitates easy coupling with conventional downstream biological assays. Device performance was optimized using cancer cell lines (> 85% recovery), followed by clinical validation with positive CTCs enumeration in all samples from patients with metastatic lung cancer (n = 20; 5-88 CTCs per mL). The presence of CD133⁺ cells, a phenotypic marker characteristic of stem-like behavior in lung cancer cells was also identified in the isolated subpopulation of CTCs. The spiral biochip identifies and addresses key challenges of the next generation CTCs isolation assay including antibody independent isolation, high sensitivity and throughput (3 mL/hr); and single-step retrieval of viable CTCs.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	NATURE PUBLISHING GROUP
dc.relation.ispartof	Sci Rep
dc.relation.isbasedon	10.1038/srep01259
dc.rights	info:eu-repo/semantics/openAccess
dc.subject.mesh	AC133 Antigen
dc.subject.mesh	Antigens, CD
dc.subject.mesh	Blood Cells
dc.subject.mesh	Cell Line, Tumor
dc.subject.mesh	Cell Separation
dc.subject.mesh	Centrifugation
dc.subject.mesh	Glycoproteins
dc.subject.mesh	Humans
dc.subject.mesh	Keratins
dc.subject.mesh	Leukocyte Common Antigens
dc.subject.mesh	Lung Neoplasms
dc.subject.mesh	MCF-7 Cells
dc.subject.mesh	Microfluidic Analytical Techniques
dc.subject.mesh	Neoplastic Cells, Circulating
dc.subject.mesh	Peptides
dc.subject.mesh	Blood Cells
dc.subject.mesh	Cell Line, Tumor
dc.subject.mesh	Humans
dc.subject.mesh	Lung Neoplasms
dc.subject.mesh	Glycoproteins
dc.subject.mesh	Peptides
dc.subject.mesh	Antigens, CD
dc.subject.mesh	Centrifugation
dc.subject.mesh	Microfluidic Analytical Techniques
dc.subject.mesh	Cell Separation
dc.subject.mesh	Keratins
dc.subject.mesh	Neoplastic Cells, Circulating
dc.subject.mesh	MCF-7 Cells
dc.subject.mesh	AC133 Antigen
dc.subject.mesh	Leukocyte Common Antigens
dc.subject.mesh	AC133 Antigen
dc.subject.mesh	Antigens, CD
dc.subject.mesh	Blood Cells
dc.subject.mesh	Cell Line, Tumor
dc.subject.mesh	Cell Separation
dc.subject.mesh	Centrifugation
dc.subject.mesh	Glycoproteins
dc.subject.mesh	Humans
dc.subject.mesh	Keratins
dc.subject.mesh	Leukocyte Common Antigens
dc.subject.mesh	Lung Neoplasms
dc.subject.mesh	MCF-7 Cells
dc.subject.mesh	Microfluidic Analytical Techniques
dc.subject.mesh	Neoplastic Cells, Circulating
dc.subject.mesh	Peptides
dc.title	Isolation and retrieval of circulating tumor cells using centrifugal forces.
dc.type	Journal Article
utslib.citation.volume	3
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	open_access	*
dc.date.updated	2023-03-12T23:04:28Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	3
utslib.citation.issue	1

Abstract:

Presence and frequency of rare circulating tumor cells (CTCs) in bloodstreams of cancer patients are pivotal to early cancer detection and treatment monitoring. Here, we use a spiral microchannel with inherent centrifugal forces for continuous, size-based separation of CTCs from blood (Dean Flow Fractionation (DFF)) which facilitates easy coupling with conventional downstream biological assays. Device performance was optimized using cancer cell lines (> 85% recovery), followed by clinical validation with positive CTCs enumeration in all samples from patients with metastatic lung cancer (n = 20; 5-88 CTCs per mL). The presence of CD133⁺ cells, a phenotypic marker characteristic of stem-like behavior in lung cancer cells was also identified in the isolated subpopulation of CTCs. The spiral biochip identifies and addresses key challenges of the next generation CTCs isolation assay including antibody independent isolation, high sensitivity and throughput (3 mL/hr); and single-step retrieval of viable CTCs.

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http://hdl.handle.net/10453/167093