Relationship between Serum Testosterone and Fracture Risk in Men: A Comparison of RIA and LC-MS/MS.
- Publisher:
- AMER ASSOC CLINICAL CHEMISTRY
- Publication Type:
- Journal Article
- Citation:
- Clin Chem, 2015, 61, (9), pp. 1182-1190
- Issue Date:
- 2015-09
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Full metadata record
Field | Value | Language |
---|---|---|
dc.contributor.author | Tran, TS | |
dc.contributor.author | Center, JR | |
dc.contributor.author | Seibel, MJ | |
dc.contributor.author | Eisman, JA | |
dc.contributor.author | Kushnir, MM | |
dc.contributor.author | Rockwood, AL | |
dc.contributor.author | Nguyen, TV | |
dc.date.accessioned | 2023-03-19T09:12:32Z | |
dc.date.available | 2015-06-09 | |
dc.date.available | 2023-03-19T09:12:32Z | |
dc.date.issued | 2015-09 | |
dc.identifier.citation | Clin Chem, 2015, 61, (9), pp. 1182-1190 | |
dc.identifier.issn | 0009-9147 | |
dc.identifier.issn | 1530-8561 | |
dc.identifier.uri | http://hdl.handle.net/10453/167557 | |
dc.description.abstract | BACKGROUND: Serum testosterone can be measured by LC-MS/MS and RIA. We investigated whether the testosterone-fracture relationship was affected by the method of measurement. METHODS: We measured total testosterone (TT) by LC-MS/MS (TTLC-MS/MS) and RIA (TTRIA) in serum samples collected from 602 men whose incident fractures had been continuously ascertained by x-ray reports from 1989 to 2010. We measured bone mineral density (BMD) by dual-energy x-ray absorptiometry. The association between TT and fracture risk was assessed by the Cox proportional hazards model, taking into account the effect of age and BMD. RESULTS: Mean TTLC-MS/MS was higher than TTRIA by 27 ng/dL (95% CI 13-41). The concordance correlation coefficient between TTLC-MS/MS and TTRIA was 0.72 (95% CI 0.68-0.76). The Deming regression equation linking the 2 measurements was ln(TTLC-MS/MS + 10) = 0.87 + 0.87 × ln(TTRIA + 10). The hazard ratio of fracture per SD decrease in TT was 1.32 (95% CI 1.12-1.54) for TTLC-MS/MS and 1.23 (1.06-1.43) for TTRIA. The correlation between predicted probabilities of fracture by TTLC-MS/MS and TTRIA was r = 0.96, with the mean difference being 0.01% (95% CI -6.1% to 6.2%). Slightly more patients were classified as having hypogonadism if TTRIA was used (29% vs 26%). CONCLUSIONS: The concordance between LC-MS/MS and RIA in the measurement of serum TT was moderate. Moreover, the magnitude of association between testosterone and fracture risk in older men was largely unaffected by the method of measurement. | |
dc.format | Print-Electronic | |
dc.language | eng | |
dc.publisher | AMER ASSOC CLINICAL CHEMISTRY | |
dc.relation.ispartof | Clin Chem | |
dc.relation.isbasedon | 10.1373/clinchem.2015.242339 | |
dc.rights | info:eu-repo/semantics/closedAccess | |
dc.subject | 1004 Medical Biotechnology, 1101 Medical Biochemistry and Metabolomics, 1103 Clinical Sciences | |
dc.subject.classification | General Clinical Medicine | |
dc.subject.mesh | Aged | |
dc.subject.mesh | Bone Density | |
dc.subject.mesh | Chromatography, Liquid | |
dc.subject.mesh | Fractures, Bone | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Male | |
dc.subject.mesh | Proportional Hazards Models | |
dc.subject.mesh | Risk Factors | |
dc.subject.mesh | Tandem Mass Spectrometry | |
dc.subject.mesh | Testosterone | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Testosterone | |
dc.subject.mesh | Chromatography, Liquid | |
dc.subject.mesh | Proportional Hazards Models | |
dc.subject.mesh | Risk Factors | |
dc.subject.mesh | Bone Density | |
dc.subject.mesh | Aged | |
dc.subject.mesh | Male | |
dc.subject.mesh | Fractures, Bone | |
dc.subject.mesh | Tandem Mass Spectrometry | |
dc.subject.mesh | Aged | |
dc.subject.mesh | Bone Density | |
dc.subject.mesh | Chromatography, Liquid | |
dc.subject.mesh | Fractures, Bone | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Male | |
dc.subject.mesh | Proportional Hazards Models | |
dc.subject.mesh | Risk Factors | |
dc.subject.mesh | Tandem Mass Spectrometry | |
dc.subject.mesh | Testosterone | |
dc.title | Relationship between Serum Testosterone and Fracture Risk in Men: A Comparison of RIA and LC-MS/MS. | |
dc.type | Journal Article | |
utslib.citation.volume | 61 | |
utslib.location.activity | England | |
utslib.for | 1101 Medical Biochemistry and Metabolomics | |
utslib.for | 1004 Medical Biotechnology | |
utslib.for | 1101 Medical Biochemistry and Metabolomics | |
utslib.for | 1103 Clinical Sciences | |
pubs.organisational-group | /University of Technology Sydney | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Engineering and Information Technology | |
pubs.organisational-group | /University of Technology Sydney/Strength - CHT - Health Technologies | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering | |
pubs.organisational-group | /University of Technology Sydney/Centre for Health Technologies (CHT) | |
utslib.copyright.status | closed_access | * |
dc.date.updated | 2023-03-19T09:12:31Z | |
pubs.issue | 9 | |
pubs.publication-status | Published | |
pubs.volume | 61 | |
utslib.citation.issue | 9 |
Abstract:
BACKGROUND: Serum testosterone can be measured by LC-MS/MS and RIA. We investigated whether the testosterone-fracture relationship was affected by the method of measurement. METHODS: We measured total testosterone (TT) by LC-MS/MS (TTLC-MS/MS) and RIA (TTRIA) in serum samples collected from 602 men whose incident fractures had been continuously ascertained by x-ray reports from 1989 to 2010. We measured bone mineral density (BMD) by dual-energy x-ray absorptiometry. The association between TT and fracture risk was assessed by the Cox proportional hazards model, taking into account the effect of age and BMD. RESULTS: Mean TTLC-MS/MS was higher than TTRIA by 27 ng/dL (95% CI 13-41). The concordance correlation coefficient between TTLC-MS/MS and TTRIA was 0.72 (95% CI 0.68-0.76). The Deming regression equation linking the 2 measurements was ln(TTLC-MS/MS + 10) = 0.87 + 0.87 × ln(TTRIA + 10). The hazard ratio of fracture per SD decrease in TT was 1.32 (95% CI 1.12-1.54) for TTLC-MS/MS and 1.23 (1.06-1.43) for TTRIA. The correlation between predicted probabilities of fracture by TTLC-MS/MS and TTRIA was r = 0.96, with the mean difference being 0.01% (95% CI -6.1% to 6.2%). Slightly more patients were classified as having hypogonadism if TTRIA was used (29% vs 26%). CONCLUSIONS: The concordance between LC-MS/MS and RIA in the measurement of serum TT was moderate. Moreover, the magnitude of association between testosterone and fracture risk in older men was largely unaffected by the method of measurement.
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