Effect of self-assembled nanofibrous silk/polycaprolactone layer on the osteoconductivity and mechanical properties of biphasic calcium phosphate scaffolds.

Roohani-Esfahani, SI; Lu, ZF; Li, JJ; Ellis-Behnke, R; Kaplan, DL; Zreiqat, H

Effect of self-assembled nanofibrous silk/polycaprolactone layer on the osteoconductivity and mechanical properties of biphasic calcium phosphate scaffolds.

Roohani-Esfahani, SI Lu, ZF Li, JJ Ellis-Behnke, R Kaplan, DL Zreiqat, H

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Publisher:: ELSEVIER SCI LTD
Publication Type:: Journal Article
Citation:: Acta Biomater, 2012, 8, (1), pp. 302-312
Issue Date:: 2012-01

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Field	Value	Language
dc.contributor.author	Roohani-Esfahani, SI
dc.contributor.author	Lu, ZF
dc.contributor.author	Li, JJ
dc.contributor.author	Ellis-Behnke, R
dc.contributor.author	Kaplan, DL
dc.contributor.author	Zreiqat, H
dc.date.accessioned	2023-03-27T06:19:46Z
dc.date.available	2011-10-06
dc.date.available	2023-03-27T06:19:46Z
dc.date.issued	2012-01
dc.identifier.citation	Acta Biomater, 2012, 8, (1), pp. 302-312
dc.identifier.issn	1742-7061
dc.identifier.issn	1878-7568
dc.identifier.uri	http://hdl.handle.net/10453/168606
dc.description.abstract	We here present the first successful report on combining nanostructured silk and poly(ε-caprolactone) (PCL) with a ceramic scaffold to produce a composite scaffold that is highly porous (porosity ∼85%, pore size ∼500 μm, ∼100% interconnectivity), strong and non-brittle with a surface that resembles extracellular matrix (ECM). The ECM-like surface was developed by self-assembly of nanofibrous structured silk (20-80 nm diameter, similar to native collagen found in ECM) over a thin PCL layer which is coated on biphasic calcium phosphate (BCP) scaffolds. The effects of different concentrations of silk solution on the mechanical and physical properties of the scaffolds were also comprehensively examined. Our results showed that using silk only (irrespective of concentration) for the modification of ceramic scaffolds could drastically reduce the compressive strength of the modified scaffolds in aqueous media, and the modification made a limited contribution to improving scaffold toughness. Using PCL/nanostructured silk the compressive strength and modulus of the modified scaffolds reached 0.42 MPa (compared with 0.07 MPa for BCP) and ∼25 MPa (compared with 5 MPa for BCP), respectively. The failure strain of the modified scaffold increased more than 6% compared with a BCP scaffold (failure strain of less than 1%), indicating a transformation from brittle to elastic behavior. The cytocompatibility of ECM-like composite scaffolds was investigated by studying the attachment, morphology, proliferation and bone-related gene expression of primary human bone-derived cells. Cells cultured on the developed scaffolds for 7 days had significant up-regulation of cell proliferation (∼1.6-fold higher, P<0.001) and osteogenic gene expression levels (collagen type I, osteocalcin and bone sialoprotein) compared with the other groups tested.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	ELSEVIER SCI LTD
dc.relation.ispartof	Acta Biomater
dc.relation.isbasedon	10.1016/j.actbio.2011.10.009
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject.classification	Biomedical Engineering
dc.subject.mesh	Apatites
dc.subject.mesh	Biocompatible Materials
dc.subject.mesh	Body Fluids
dc.subject.mesh	Bone Regeneration
dc.subject.mesh	Bone and Bones
dc.subject.mesh	Calcium Phosphates
dc.subject.mesh	Cells, Cultured
dc.subject.mesh	Ceramics
dc.subject.mesh	Compressive Strength
dc.subject.mesh	Elasticity
dc.subject.mesh	Extracellular Matrix
dc.subject.mesh	Gene Expression
dc.subject.mesh	Humans
dc.subject.mesh	Materials Testing
dc.subject.mesh	Nanofibers
dc.subject.mesh	Osteoblasts
dc.subject.mesh	Polyesters
dc.subject.mesh	Porosity
dc.subject.mesh	Silk
dc.subject.mesh	Stress, Mechanical
dc.subject.mesh	Tissue Engineering
dc.subject.mesh	Tissue Scaffolds
dc.subject.mesh	Bone and Bones
dc.subject.mesh	Cells, Cultured
dc.subject.mesh	Extracellular Matrix
dc.subject.mesh	Osteoblasts
dc.subject.mesh	Body Fluids
dc.subject.mesh	Humans
dc.subject.mesh	Calcium Phosphates
dc.subject.mesh	Apatites
dc.subject.mesh	Silk
dc.subject.mesh	Polyesters
dc.subject.mesh	Biocompatible Materials
dc.subject.mesh	Ceramics
dc.subject.mesh	Tissue Engineering
dc.subject.mesh	Materials Testing
dc.subject.mesh	Bone Regeneration
dc.subject.mesh	Gene Expression
dc.subject.mesh	Compressive Strength
dc.subject.mesh	Elasticity
dc.subject.mesh	Stress, Mechanical
dc.subject.mesh	Porosity
dc.subject.mesh	Tissue Scaffolds
dc.subject.mesh	Nanofibers
dc.subject.mesh	Apatites
dc.subject.mesh	Biocompatible Materials
dc.subject.mesh	Body Fluids
dc.subject.mesh	Bone Regeneration
dc.subject.mesh	Bone and Bones
dc.subject.mesh	Calcium Phosphates
dc.subject.mesh	Cells, Cultured
dc.subject.mesh	Ceramics
dc.subject.mesh	Compressive Strength
dc.subject.mesh	Elasticity
dc.subject.mesh	Extracellular Matrix
dc.subject.mesh	Gene Expression
dc.subject.mesh	Humans
dc.subject.mesh	Materials Testing
dc.subject.mesh	Nanofibers
dc.subject.mesh	Osteoblasts
dc.subject.mesh	Polyesters
dc.subject.mesh	Porosity
dc.subject.mesh	Silk
dc.subject.mesh	Stress, Mechanical
dc.subject.mesh	Tissue Engineering
dc.subject.mesh	Tissue Scaffolds
dc.title	Effect of self-assembled nanofibrous silk/polycaprolactone layer on the osteoconductivity and mechanical properties of biphasic calcium phosphate scaffolds.
dc.type	Journal Article
utslib.citation.volume	8
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
utslib.copyright.status	closed_access	*
dc.date.updated	2023-03-27T06:19:44Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	8
utslib.citation.issue	1

Abstract:

We here present the first successful report on combining nanostructured silk and poly(ε-caprolactone) (PCL) with a ceramic scaffold to produce a composite scaffold that is highly porous (porosity ∼85%, pore size ∼500 μm, ∼100% interconnectivity), strong and non-brittle with a surface that resembles extracellular matrix (ECM). The ECM-like surface was developed by self-assembly of nanofibrous structured silk (20-80 nm diameter, similar to native collagen found in ECM) over a thin PCL layer which is coated on biphasic calcium phosphate (BCP) scaffolds. The effects of different concentrations of silk solution on the mechanical and physical properties of the scaffolds were also comprehensively examined. Our results showed that using silk only (irrespective of concentration) for the modification of ceramic scaffolds could drastically reduce the compressive strength of the modified scaffolds in aqueous media, and the modification made a limited contribution to improving scaffold toughness. Using PCL/nanostructured silk the compressive strength and modulus of the modified scaffolds reached 0.42 MPa (compared with 0.07 MPa for BCP) and ∼25 MPa (compared with 5 MPa for BCP), respectively. The failure strain of the modified scaffold increased more than 6% compared with a BCP scaffold (failure strain of less than 1%), indicating a transformation from brittle to elastic behavior. The cytocompatibility of ECM-like composite scaffolds was investigated by studying the attachment, morphology, proliferation and bone-related gene expression of primary human bone-derived cells. Cells cultured on the developed scaffolds for 7 days had significant up-regulation of cell proliferation (∼1.6-fold higher, P<0.001) and osteogenic gene expression levels (collagen type I, osteocalcin and bone sialoprotein) compared with the other groups tested.

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