Multiple silk coatings on biphasic calcium phosphate scaffolds: effect on physical and mechanical properties and in vitro osteogenic response of human mesenchymal stem cells.

Li, JJ; Gil, ES; Hayden, RS; Li, C; Roohani-Esfahani, S-I; Kaplan, DL; Zreiqat, H

Multiple silk coatings on biphasic calcium phosphate scaffolds: effect on physical and mechanical properties and in vitro osteogenic response of human mesenchymal stem cells.

Li, JJ Gil, ES Hayden, RS Li, C Roohani-Esfahani, S-I Kaplan, DL Zreiqat, H

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Publisher:: AMER CHEMICAL SOC
Publication Type:: Journal Article
Citation:: Biomacromolecules, 2013, 14, (7), pp. 2179-2188
Issue Date:: 2013-07-08

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Field	Value	Language
dc.contributor.author	Li, JJ
dc.contributor.author	Gil, ES
dc.contributor.author	Hayden, RS
dc.contributor.author	Li, C
dc.contributor.author	Roohani-Esfahani, S-I
dc.contributor.author	Kaplan, DL
dc.contributor.author	Zreiqat, H
dc.date.accessioned	2023-03-27T06:23:25Z
dc.date.available	2023-03-27T06:23:25Z
dc.date.issued	2013-07-08
dc.identifier.citation	Biomacromolecules, 2013, 14, (7), pp. 2179-2188
dc.identifier.issn	1525-7797
dc.identifier.issn	1526-4602
dc.identifier.uri	http://hdl.handle.net/10453/168608
dc.description.abstract	Ceramic scaffolds such as biphasic calcium phosphate (BCP) have been widely studied and used for bone regeneration, but their brittleness and low mechanical strength are major drawbacks. We report the first systematic study on the effect of silk coating in improving the mechanical and biological properties of BCP scaffolds, including (1) optimization of the silk coating process by investigating multiple coatings, and (2) in vitro evaluation of the osteogenic response of human mesenchymal stem cells (hMSCs) on the coated scaffolds. Our results show that multiple silk coatings on BCP ceramic scaffolds can achieve a significant coating effect to approach the mechanical properties of native bone tissue and positively influence osteogenesis by hMSCs over an extended period. The silk coating method developed in this study represents a simple yet effective means of reinforcement that can be applied to other types of ceramic scaffolds with similar microstructure to improve osteogenic outcomes.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	AMER CHEMICAL SOC
dc.relation.ispartof	Biomacromolecules
dc.relation.isbasedon	10.1021/bm400303w
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	03 Chemical Sciences, 06 Biological Sciences, 09 Engineering
dc.subject.classification	Polymers
dc.subject.mesh	Adult
dc.subject.mesh	Alkaline Phosphatase
dc.subject.mesh	Bone Regeneration
dc.subject.mesh	Cell Differentiation
dc.subject.mesh	Cell Proliferation
dc.subject.mesh	Cells, Cultured
dc.subject.mesh	Ceramics
dc.subject.mesh	Collagen Type I
dc.subject.mesh	Gene Expression
dc.subject.mesh	Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+)
dc.subject.mesh	Humans
dc.subject.mesh	Hydroxyapatites
dc.subject.mesh	Integrin-Binding Sialoprotein
dc.subject.mesh	Male
dc.subject.mesh	Materials Testing
dc.subject.mesh	Mesenchymal Stem Cells
dc.subject.mesh	Osteoblasts
dc.subject.mesh	Osteogenesis
dc.subject.mesh	Porosity
dc.subject.mesh	Silk
dc.subject.mesh	Tissue Engineering
dc.subject.mesh	Tissue Scaffolds
dc.subject.mesh	Cells, Cultured
dc.subject.mesh	Osteoblasts
dc.subject.mesh	Mesenchymal Stem Cells
dc.subject.mesh	Humans
dc.subject.mesh	Hydroxyapatites
dc.subject.mesh	Collagen Type I
dc.subject.mesh	Silk
dc.subject.mesh	Alkaline Phosphatase
dc.subject.mesh	Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+)
dc.subject.mesh	Ceramics
dc.subject.mesh	Tissue Engineering
dc.subject.mesh	Materials Testing
dc.subject.mesh	Bone Regeneration
dc.subject.mesh	Cell Differentiation
dc.subject.mesh	Cell Proliferation
dc.subject.mesh	Gene Expression
dc.subject.mesh	Osteogenesis
dc.subject.mesh	Porosity
dc.subject.mesh	Adult
dc.subject.mesh	Male
dc.subject.mesh	Tissue Scaffolds
dc.subject.mesh	Integrin-Binding Sialoprotein
dc.subject.mesh	Adult
dc.subject.mesh	Alkaline Phosphatase
dc.subject.mesh	Bone Regeneration
dc.subject.mesh	Cell Differentiation
dc.subject.mesh	Cell Proliferation
dc.subject.mesh	Cells, Cultured
dc.subject.mesh	Ceramics
dc.subject.mesh	Collagen Type I
dc.subject.mesh	Gene Expression
dc.subject.mesh	Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+)
dc.subject.mesh	Humans
dc.subject.mesh	Hydroxyapatites
dc.subject.mesh	Integrin-Binding Sialoprotein
dc.subject.mesh	Male
dc.subject.mesh	Materials Testing
dc.subject.mesh	Mesenchymal Stem Cells
dc.subject.mesh	Osteoblasts
dc.subject.mesh	Osteogenesis
dc.subject.mesh	Porosity
dc.subject.mesh	Silk
dc.subject.mesh	Tissue Engineering
dc.subject.mesh	Tissue Scaffolds
dc.title	Multiple silk coatings on biphasic calcium phosphate scaffolds: effect on physical and mechanical properties and in vitro osteogenic response of human mesenchymal stem cells.
dc.type	Journal Article
utslib.citation.volume	14
utslib.location.activity	United States
utslib.for	03 Chemical Sciences
utslib.for	06 Biological Sciences
utslib.for	09 Engineering
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
utslib.copyright.status	closed_access	*
dc.date.updated	2023-03-27T06:23:20Z
pubs.issue	7
pubs.publication-status	Published
pubs.volume	14
utslib.citation.issue	7

Abstract:

Ceramic scaffolds such as biphasic calcium phosphate (BCP) have been widely studied and used for bone regeneration, but their brittleness and low mechanical strength are major drawbacks. We report the first systematic study on the effect of silk coating in improving the mechanical and biological properties of BCP scaffolds, including (1) optimization of the silk coating process by investigating multiple coatings, and (2) in vitro evaluation of the osteogenic response of human mesenchymal stem cells (hMSCs) on the coated scaffolds. Our results show that multiple silk coatings on BCP ceramic scaffolds can achieve a significant coating effect to approach the mechanical properties of native bone tissue and positively influence osteogenesis by hMSCs over an extended period. The silk coating method developed in this study represents a simple yet effective means of reinforcement that can be applied to other types of ceramic scaffolds with similar microstructure to improve osteogenic outcomes.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/168608