In vitro testing and efficacy of poly-lactic acid coating incorporating antibiotic loaded coralline bioceramic on Ti6Al4V implant against Staphylococcus aureus.

Karacan, I; Ben-Nissan, B; Santos, J; Yiu, S; Bradbury, P; Valenzuela, SM; Chou, J

In vitro testing and efficacy of poly-lactic acid coating incorporating antibiotic loaded coralline bioceramic on Ti6Al4V implant against Staphylococcus aureus.

Karacan, I Ben-Nissan, B Santos, J

Yiu, S Bradbury, P Valenzuela, SM Chou, J

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Publisher:: WILEY
Publication Type:: Journal Article
Citation:: J Tissue Eng Regen Med, 2022, 16, (12), pp. 1149-1162
Issue Date:: 2022-12

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Field	Value	Language
dc.contributor.author	Karacan, I
dc.contributor.author	Ben-Nissan, B
dc.contributor.author	Santos, J https://orcid.org/0000-0003-4067-220X
dc.contributor.author	Yiu, S
dc.contributor.author	Bradbury, P
dc.contributor.author	Valenzuela, SM
dc.contributor.author	Chou, J https://orcid.org/0000-0002-7472-8016
dc.date.accessioned	2023-04-26T04:08:05Z
dc.date.available	2022-09-27
dc.date.available	2023-04-26T04:08:05Z
dc.date.issued	2022-12
dc.identifier.citation	J Tissue Eng Regen Med, 2022, 16, (12), pp. 1149-1162
dc.identifier.issn	1932-6254
dc.identifier.issn	1932-7005
dc.identifier.uri	http://hdl.handle.net/10453/170105
dc.description.abstract	Biofilm formation on an implant surface is most commonly caused by the human pathogenic bacteria Staphylococcus aureus, which can lead to implant related infections and failure. It is a major problem for both implantable orthopedic and maxillofacial devices. The current antibiotic treatments are typically delivered orally or in an injectable form. They are not highly effective in preventing or removing biofilms, and they increase the risk of antibiotic resistance of bacteria and have a dose-dependent negative biological effect on human cells. Our aim was to improve current treatments via a localized and controlled antibiotic delivery-based implant coating system to deliver the antibiotic, gentamicin (Gm). The coating contains coral skeleton derived hydroxyapatite powders (HAp) that act as antibiotic carrier particles and have a biodegradable poly-lactic acid (PLA) thin film matrix. The system is designed to prevent implant related infections while avoiding the deleterious effects of high concentration antibiotics in implants on local cells including primary human adipose derived stem cells (ADSCs). Testing undertaken in this study measured the rate of S. aureus biofilm formation and determined the growth rate and proliferation of ADSCs. After 24 h, S. aureus biofilm formation and the percentage of live cells found on the surfaces of all 5%-30% (w/w) PLA-Gm-(HAp-Gm) coated Ti6Al4V implants was lower than the control samples. Furthermore, Ti6Al4V implants coated with up to 10% (w/w) PLA-Gm-(HAp-Gm) did not have noticeable Gm related adverse effect on ADSCs, as assessed by morphological and surface attachment analyses. These results support the use and application of the antibacterial PLA-Gm-(HAp-Gm) thin film coating design for implants, as an antibiotic release control mechanism to prevent implant-related infections.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	WILEY
dc.relation.ispartof	J Tissue Eng Regen Med
dc.relation.isbasedon	10.1002/term.3353
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0903 Biomedical Engineering, 1103 Clinical Sciences, 1116 Medical Physiology
dc.subject.classification	Biomedical Engineering
dc.subject.mesh	Humans
dc.subject.mesh	Staphylococcus aureus
dc.subject.mesh	Anti-Bacterial Agents
dc.subject.mesh	Coated Materials, Biocompatible
dc.subject.mesh	Staphylococcal Infections
dc.subject.mesh	Gentamicins
dc.subject.mesh	Polyesters
dc.subject.mesh	In Vitro Techniques
dc.subject.mesh	Lactic Acid
dc.subject.mesh	Humans
dc.subject.mesh	Staphylococcus aureus
dc.subject.mesh	Staphylococcal Infections
dc.subject.mesh	Lactic Acid
dc.subject.mesh	Polyesters
dc.subject.mesh	Gentamicins
dc.subject.mesh	Coated Materials, Biocompatible
dc.subject.mesh	Anti-Bacterial Agents
dc.subject.mesh	In Vitro Techniques
dc.subject.mesh	Humans
dc.subject.mesh	Staphylococcus aureus
dc.subject.mesh	Anti-Bacterial Agents
dc.subject.mesh	Coated Materials, Biocompatible
dc.subject.mesh	Staphylococcal Infections
dc.subject.mesh	Gentamicins
dc.subject.mesh	Polyesters
dc.subject.mesh	In Vitro Techniques
dc.subject.mesh	Lactic Acid
dc.title	In vitro testing and efficacy of poly-lactic acid coating incorporating antibiotic loaded coralline bioceramic on Ti6Al4V implant against Staphylococcus aureus.
dc.type	Journal Article
utslib.citation.volume	16
utslib.location.activity	England
utslib.for	0903 Biomedical Engineering
utslib.for	1103 Clinical Sciences
utslib.for	1116 Medical Physiology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	closed_access	*
dc.date.updated	2023-04-26T04:08:04Z
pubs.issue	12
pubs.publication-status	Published
pubs.volume	16
utslib.citation.issue	12

Abstract:

Biofilm formation on an implant surface is most commonly caused by the human pathogenic bacteria Staphylococcus aureus, which can lead to implant related infections and failure. It is a major problem for both implantable orthopedic and maxillofacial devices. The current antibiotic treatments are typically delivered orally or in an injectable form. They are not highly effective in preventing or removing biofilms, and they increase the risk of antibiotic resistance of bacteria and have a dose-dependent negative biological effect on human cells. Our aim was to improve current treatments via a localized and controlled antibiotic delivery-based implant coating system to deliver the antibiotic, gentamicin (Gm). The coating contains coral skeleton derived hydroxyapatite powders (HAp) that act as antibiotic carrier particles and have a biodegradable poly-lactic acid (PLA) thin film matrix. The system is designed to prevent implant related infections while avoiding the deleterious effects of high concentration antibiotics in implants on local cells including primary human adipose derived stem cells (ADSCs). Testing undertaken in this study measured the rate of S. aureus biofilm formation and determined the growth rate and proliferation of ADSCs. After 24 h, S. aureus biofilm formation and the percentage of live cells found on the surfaces of all 5%-30% (w/w) PLA-Gm-(HAp-Gm) coated Ti6Al4V implants was lower than the control samples. Furthermore, Ti6Al4V implants coated with up to 10% (w/w) PLA-Gm-(HAp-Gm) did not have noticeable Gm related adverse effect on ADSCs, as assessed by morphological and surface attachment analyses. These results support the use and application of the antibacterial PLA-Gm-(HAp-Gm) thin film coating design for implants, as an antibiotic release control mechanism to prevent implant-related infections.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/170105