Genetic and morphological identification of formalin fixed, preserved larval fishes; can we have the best of both worlds?

Appleyard, SA; Maher, S; Miskiewicz, AG; Lara-Lopez, A; Matis, P; Fielder, DS; Suthers, IM

Genetic and morphological identification of formalin fixed, preserved larval fishes; can we have the best of both worlds?

Appleyard, SA Maher, S Miskiewicz, AG Lara-Lopez, A Matis, P Fielder, DS Suthers, IM

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Publisher:: Elsevier
Publication Type:: Journal Article
Citation:: Journal of Experimental Marine Biology and Ecology, 2022, 553, pp. 1-10
Issue Date:: 2022-08-01

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Field	Value	Language
dc.contributor.author	Appleyard, SA
dc.contributor.author	Maher, S
dc.contributor.author	Miskiewicz, AG
dc.contributor.author	Lara-Lopez, A
dc.contributor.author	Matis, P
dc.contributor.author	Fielder, DS
dc.contributor.author	Suthers, IM
dc.date.accessioned	2023-06-19T20:28:31Z
dc.date.available	2023-06-19T20:28:31Z
dc.date.issued	2022-08-01
dc.identifier.citation	Journal of Experimental Marine Biology and Ecology, 2022, 553, pp. 1-10
dc.identifier.issn	0022-0981
dc.identifier.uri	http://hdl.handle.net/10453/170797
dc.description.abstract	Surveys of larval fishes require accurate identifications of larvae which are essential to understand early life history of fish fish ecology and fisheries However the identification of larval fishes requires microscopic examination that is substantially more difficult than that of juvenile and adult fishes as many larval stages remain undescribed Furthermore the traditional formalin fixation of larval fishes were previously thought to prevent genetic sequencing compared to ethanol preserved larvae In this study we used an integrative taxonomic approach based on morphology imaging and DNA barcoding of the mitochondrial mtDNA cytochrome c oxidase subunit COI gene We used this approach in both cultured yellowtail kingfish Seriola lalandi and wild sourced fish larvae that had been fixed in 5 formalin Based on controlled and in field formalin treatments DNA barcoding and genetic species identification was 100 successful in cultured yellowtail kingfish fixed in formalin for up to 6 months while barcoding of wild caught fish larvae enabled species identification of 93 of up to 8 weeks formalin fixed specimens Furthermore we demonstrated the viability of using either whole larval individuals or a single eyeball removed
dc.language	en
dc.publisher	Elsevier
dc.relation.ispartof	Journal of Experimental Marine Biology and Ecology
dc.relation.isbasedon	10.1016/j.jembe.2022.151763
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	05 Environmental Sciences, 06 Biological Sciences, 07 Agricultural and Veterinary Sciences
dc.subject.classification	Marine Biology & Hydrobiology
dc.title	Genetic and morphological identification of formalin fixed, preserved larval fishes; can we have the best of both worlds?
dc.type	Journal Article
utslib.citation.volume	553
utslib.for	05 Environmental Sciences
utslib.for	06 Biological Sciences
utslib.for	07 Agricultural and Veterinary Sciences
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	open_access	*
pubs.consider-herdc	false
dc.date.updated	2023-06-19T20:28:29Z
pubs.publication-status	Published
pubs.volume	553

Abstract:

Surveys of larval fishes require accurate identifications of larvae which are essential to understand early life history of fish fish ecology and fisheries However the identification of larval fishes requires microscopic examination that is substantially more difficult than that of juvenile and adult fishes as many larval stages remain undescribed Furthermore the traditional formalin fixation of larval fishes were previously thought to prevent genetic sequencing compared to ethanol preserved larvae In this study we used an integrative taxonomic approach based on morphology imaging and DNA barcoding of the mitochondrial mtDNA cytochrome c oxidase subunit COI gene We used this approach in both cultured yellowtail kingfish Seriola lalandi and wild sourced fish larvae that had been fixed in 5 formalin Based on controlled and in field formalin treatments DNA barcoding and genetic species identification was 100 successful in cultured yellowtail kingfish fixed in formalin for up to 6 months while barcoding of wild caught fish larvae enabled species identification of 93 of up to 8 weeks formalin fixed specimens Furthermore we demonstrated the viability of using either whole larval individuals or a single eyeball removed

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http://hdl.handle.net/10453/170797