Decellularized human amniotic membrane reinforced by MoS2-Polycaprolactone nanofibers, a novel conductive scaffold for cardiac tissue engineering.

Nazari, H; Heirani-Tabasi, A; Esmaeili, E; Kajbafzadeh, A-M; Hassannejad, Z; Boroomand, S; Shahsavari Alavijeh, MH; Mishan, MA; Ahmadi Tafti, SH; Warkiani, ME; Dadgar, N

Decellularized human amniotic membrane reinforced by MoS2-Polycaprolactone nanofibers, a novel conductive scaffold for cardiac tissue engineering.

Nazari, H Heirani-Tabasi, A Esmaeili, E Kajbafzadeh, A-M Hassannejad, Z Boroomand, S Shahsavari Alavijeh, MH Mishan, MA Ahmadi Tafti, SH Warkiani, ME Dadgar, N

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Publisher:: SAGE Publications
Publication Type:: Journal Article
Citation:: J Biomater Appl, 2022, 36, (9), pp. 1527-1539
Issue Date:: 2022-04

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Field	Value	Language
dc.contributor.author	Nazari, H
dc.contributor.author	Heirani-Tabasi, A
dc.contributor.author	Esmaeili, E
dc.contributor.author	Kajbafzadeh, A-M
dc.contributor.author	Hassannejad, Z
dc.contributor.author	Boroomand, S
dc.contributor.author	Shahsavari Alavijeh, MH
dc.contributor.author	Mishan, MA
dc.contributor.author	Ahmadi Tafti, SH
dc.contributor.author	Warkiani, ME
dc.contributor.author	Dadgar, N
dc.date.accessioned	2023-06-29T06:46:28Z
dc.date.available	2023-06-29T06:46:28Z
dc.date.issued	2022-04
dc.identifier.citation	J Biomater Appl, 2022, 36, (9), pp. 1527-1539
dc.identifier.issn	0885-3282
dc.identifier.issn	1530-8022
dc.identifier.uri	http://hdl.handle.net/10453/171008
dc.description.abstract	In order to regenerate myocardial tissues with functional characteristics, we need to copy some properties of the myocardium, such as its extracellular matrix and electrical conductivity. In this study, we synthesized nanosheets of Molybdenum disulfide (MoS2), and integrated them into polycaprolactone (PCL) and electrospun on the surface of decellularized human amniotic membrane (DHAM) with the purpose of improving the scaffolds mechanical properties and electrical conductivity. For in vitro studies, we seeded the mouse embryonic cardiac cells, mouse Embryonic Cardiac Cells (mECCs), on the scaffolds and then studied the MoS2 nanocomposites by scanning electron microscopy and Raman spectroscopy. In addition, we characterized the DHAM/PCL and DHAM/PCL-MoS2 by SEM, transmission electron microscopy, water contact angle measurement, electrical conductivity, and tensile test. Besides, we confirmed the scaffolds are biocompatible by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, MTT assay. Furthermore, by means of SEM images, it was shown that mECCs attached to the DHAM/PCL-MoS2 scaffold have more cell aggregations and elongated morphology. Furthermore, through the Real-Time PCR and immunostaining studies, we found out cardiac genes were maturated and upregulated, and they also included GATA-4, c-TnT, NKX 2.5, and alpha-myosin heavy chain in cells cultured on DHAM/PCL-MoS2 scaffold in comparison to DHAM/PCL and DHAM. Therefore, in terms of cardiac tissue engineering, DHAM nanofibrous scaffolds reinforced by PCL-MoS2 can be suggested as a proper candidate.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	SAGE Publications
dc.relation.ispartof	J Biomater Appl
dc.relation.isbasedon	10.1177/08853282211063289
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0903 Biomedical Engineering, 0912 Materials Engineering
dc.subject.classification	Biomedical Engineering
dc.subject.mesh	Amnion
dc.subject.mesh	Animals
dc.subject.mesh	Cell Proliferation
dc.subject.mesh	Electric Conductivity
dc.subject.mesh	Humans
dc.subject.mesh	Mice
dc.subject.mesh	Molybdenum
dc.subject.mesh	Nanofibers
dc.subject.mesh	Polyesters
dc.subject.mesh	Tissue Engineering
dc.subject.mesh	Tissue Scaffolds
dc.subject.mesh	Amnion
dc.subject.mesh	Animals
dc.subject.mesh	Humans
dc.subject.mesh	Mice
dc.subject.mesh	Molybdenum
dc.subject.mesh	Polyesters
dc.subject.mesh	Tissue Engineering
dc.subject.mesh	Cell Proliferation
dc.subject.mesh	Electric Conductivity
dc.subject.mesh	Tissue Scaffolds
dc.subject.mesh	Nanofibers
dc.subject.mesh	Amnion
dc.subject.mesh	Animals
dc.subject.mesh	Cell Proliferation
dc.subject.mesh	Electric Conductivity
dc.subject.mesh	Humans
dc.subject.mesh	Mice
dc.subject.mesh	Molybdenum
dc.subject.mesh	Nanofibers
dc.subject.mesh	Polyesters
dc.subject.mesh	Tissue Engineering
dc.subject.mesh	Tissue Scaffolds
dc.title	Decellularized human amniotic membrane reinforced by MoS2-Polycaprolactone nanofibers, a novel conductive scaffold for cardiac tissue engineering.
dc.type	Journal Article
utslib.citation.volume	36
utslib.location.activity	England
utslib.for	0903 Biomedical Engineering
utslib.for	0912 Materials Engineering
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	closed_access	*
dc.date.updated	2023-06-29T06:46:26Z
pubs.issue	9
pubs.publication-status	Published
pubs.volume	36
utslib.citation.issue	9

Abstract:

In order to regenerate myocardial tissues with functional characteristics, we need to copy some properties of the myocardium, such as its extracellular matrix and electrical conductivity. In this study, we synthesized nanosheets of Molybdenum disulfide (MoS2), and integrated them into polycaprolactone (PCL) and electrospun on the surface of decellularized human amniotic membrane (DHAM) with the purpose of improving the scaffolds mechanical properties and electrical conductivity. For in vitro studies, we seeded the mouse embryonic cardiac cells, mouse Embryonic Cardiac Cells (mECCs), on the scaffolds and then studied the MoS2 nanocomposites by scanning electron microscopy and Raman spectroscopy. In addition, we characterized the DHAM/PCL and DHAM/PCL-MoS2 by SEM, transmission electron microscopy, water contact angle measurement, electrical conductivity, and tensile test. Besides, we confirmed the scaffolds are biocompatible by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, MTT assay. Furthermore, by means of SEM images, it was shown that mECCs attached to the DHAM/PCL-MoS2 scaffold have more cell aggregations and elongated morphology. Furthermore, through the Real-Time PCR and immunostaining studies, we found out cardiac genes were maturated and upregulated, and they also included GATA-4, c-TnT, NKX 2.5, and alpha-myosin heavy chain in cells cultured on DHAM/PCL-MoS2 scaffold in comparison to DHAM/PCL and DHAM. Therefore, in terms of cardiac tissue engineering, DHAM nanofibrous scaffolds reinforced by PCL-MoS2 can be suggested as a proper candidate.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/171008