Creatine and creatinine quantification in olympic athletes: dried blood spot analysis pilot study.

Bachini, FI; Pereira, D; Santos, R; Hausen, M; Pereira, G; Vieira, C; Taylor, L; Pegurier, M

Creatine and creatinine quantification in olympic athletes: dried blood spot analysis pilot study.

Bachini, FI Pereira, D Santos, R Hausen, M Pereira, G Vieira, C Taylor, L Pegurier, M

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Publisher:: Termedia Publishing House
Publication Type:: Journal Article
Citation:: Biology of Sport, 2022, 39, (3), pp. 745-749
Issue Date:: 2022-09

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Field	Value	Language
dc.contributor.author	Bachini, FI
dc.contributor.author	Pereira, D
dc.contributor.author	Santos, R
dc.contributor.author	Hausen, M
dc.contributor.author	Pereira, G
dc.contributor.author	Vieira, C
dc.contributor.author	Taylor, L
dc.contributor.author	Pegurier, M
dc.date.accessioned	2023-07-11T05:07:13Z
dc.date.available	2021-07-16
dc.date.available	2023-07-11T05:07:13Z
dc.date.issued	2022-09
dc.identifier.citation	Biology of Sport, 2022, 39, (3), pp. 745-749
dc.identifier.issn	0860-021X
dc.identifier.issn	2083-1862
dc.identifier.uri	http://hdl.handle.net/10453/171446
dc.description.abstract	Capillary dried blood spot (DBS) samples facilitate field-based collection without venipuncture. This pilot study aims to evaluate the viability of creatine (Cr) and creatinine (Crt) quantification using fresh capillary serum (CrS/CrtS) and DBS samples (CrDBS/CrtDBS), using Flow Injection Analysis Mass Spectrometry (FIA - MS). Nine Olympic Athletes provided a capillary blood sample to assess CrS/CrtS and CrDBS/CrtDBS quantified by FIA - MS. No difference between CrtS (mean ± SD: 813.6 ± 102.4 μmol/L) and CrtDBS (812.4 ± 108.1 μmol/L) was observed with acceptable variance [SEM 88.7; CV 10.7%; ICC 0.57 (CI 95% 0.06 - 0.84)] and agreement [very strong (Spearman: r = 0.77; p < 0.01) or strong (Pearson: r = 0.56; p = 0.04); Bland Altman: lower (-193) and upper (+196) limits of agreement]. CrS (mean ± SD: 691.8 ± 165.2 μmol/L) was significantly different to CrDBS (2911 ± 571.4 μmol/L) with unacceptable variance [SEM 171.6; CV 27%; ICC 0.002 (CI 95% -0.02 - 0.07)] and 'weak' agreement [Spearman: r = 0.21, p = 0.47 and Pearson: r = 0.06, p = 0.84; Bland Altman lower (-3367) and upper (-1072) limits of agreement]. Crt quantification is viable using both CrtS and CrtDBS (but not for Cr and CrS/CrDBS), with the DBS tissue handling technique offering several methodological and practice facing advantages. Future work should expand upon the sample size, explore sport/discipline relevant analytes across a full competitive season, including key training, recovery and performance blocks of their periodized performance plan.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Termedia Publishing House
dc.relation.ispartof	Biology of Sport
dc.relation.isbasedon	10.5114/biolsport.2022.108701
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	1106 Human Movement and Sports Sciences
dc.subject.classification	Sport Sciences
dc.subject.classification	3202 Clinical sciences
dc.subject.classification	4207 Sports science and exercise
dc.title	Creatine and creatinine quantification in olympic athletes: dried blood spot analysis pilot study.
dc.type	Journal Article
utslib.citation.volume	39
utslib.location.activity	Poland
utslib.for	1106 Human Movement and Sports Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Health
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2023-07-11T05:07:11Z
pubs.issue	3
pubs.publication-status	Published
pubs.volume	39
utslib.citation.issue	3

Abstract:

Capillary dried blood spot (DBS) samples facilitate field-based collection without venipuncture. This pilot study aims to evaluate the viability of creatine (Cr) and creatinine (Crt) quantification using fresh capillary serum (CrS/CrtS) and DBS samples (CrDBS/CrtDBS), using Flow Injection Analysis Mass Spectrometry (FIA - MS). Nine Olympic Athletes provided a capillary blood sample to assess CrS/CrtS and CrDBS/CrtDBS quantified by FIA - MS. No difference between CrtS (mean ± SD: 813.6 ± 102.4 μmol/L) and CrtDBS (812.4 ± 108.1 μmol/L) was observed with acceptable variance [SEM 88.7; CV 10.7%; ICC 0.57 (CI 95% 0.06 - 0.84)] and agreement [very strong (Spearman: r = 0.77; p < 0.01) or strong (Pearson: r = 0.56; p = 0.04); Bland Altman: lower (-193) and upper (+196) limits of agreement]. CrS (mean ± SD: 691.8 ± 165.2 μmol/L) was significantly different to CrDBS (2911 ± 571.4 μmol/L) with unacceptable variance [SEM 171.6; CV 27%; ICC 0.002 (CI 95% -0.02 - 0.07)] and 'weak' agreement [Spearman: r = 0.21, p = 0.47 and Pearson: r = 0.06, p = 0.84; Bland Altman lower (-3367) and upper (-1072) limits of agreement]. Crt quantification is viable using both CrtS and CrtDBS (but not for Cr and CrS/CrDBS), with the DBS tissue handling technique offering several methodological and practice facing advantages. Future work should expand upon the sample size, explore sport/discipline relevant analytes across a full competitive season, including key training, recovery and performance blocks of their periodized performance plan.

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http://hdl.handle.net/10453/171446